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质量水平
ligand
(Sulfoisobutyl)
描述
strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
无菌性
sterile (Caustic Stable)
产品线
Fractogel®
形式
resin
特殊分析物
MAbs
参数
8 bar max. pressure
基质活性基团
methacrylate
平均粒径
40-90 μm
容量
130 mg binding capacity (lysozyme/ml of resin)
转变温度
flash point 35 °C (Does not sustain combustion.)
密度
1.43 g/cm3 at 20 °C
堆积密度
1000 kg/m3
应用
recombinant protein
vaccine development
分离技术
cation exchange
strong cation exchange
储存温度
2-30°C
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一般描述
Strong cation exchange Fractogel® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides
特点和优势
Fractogel® EMD SO3- (M) enables:
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- Increased binding strength at low pH
- High aggregate removal efficiency combined with high dynamic binding capacity
- Lower elution volumes for the highest purity levels
- Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution
Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).
The strength of the binding depends on the following:
- the buffer system
- pH value of the buffer which determines the surface charge of the protein
- the concentration of the counter ions
- the charge density on the support (protein binding capacity)
包装
- 1.16882.0100: Fractogel® EMD SO3- (M) Resin 100ml
- 1.16882.0010: Fractogel® EMD SO3- (M) Resin 10ml
- 1.16880.0500: Fractogel® EMD SO3- (M) Resin 500ml
- 1.16882.5000: Fractogel® EMD SO3- (M) Resin 5L
Plastic bottle
分析说明
Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates, no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 110 - 150 mg/ml
Functional test (b:a): ≤ 0.4
Functional test (c:d): ≤ 0.45
Functional test: Separation chymotrypsinogen A, cytochrom C and lysozyme
Microscopic evaluation: Uniform spherical particles,no agglomerates, no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 110 - 150 mg/ml
Functional test (b:a): ≤ 0.4
Functional test (c:d): ≤ 0.45
Functional test: Separation chymotrypsinogen A, cytochrom C and lysozyme
法律信息
FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany
警示用语:
Warning
危险声明
危险分类
Flam. Liq. 3
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
95.0 °F
闪点(°C)
35 °C
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