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Merck
CN

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93337

Sigma-Aldrich

Trizma®乙酸盐

BioUltra, ≥99.0% (NT)

别名:

TRIS 乙酸盐, 三(羟甲基)氨基甲烷 乙酸盐

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25 G
CN¥1,541.64
100 G
CN¥4,201.18

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25 G
CN¥1,541.64
100 G
CN¥4,201.18

About This Item

线性分子式:
NH2C(CH2OH)3 · CH3COOH
CAS Number:
分子量:
181.19
Beilstein:
3702918
EC 号:
MDL编号:
UNSPSC代码:
12161700
PubChem化学物质编号:
NACRES:
NA.25

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产品线

BioUltra

质量水平

方案

≥99.0% (NT)

表单

crystalline powder

杂质

insoluble matter, passes filter test

灼烧残渣

≤0.2%

缺失

≤0.2% loss on drying, 20 °C (HV)

pH值(酸碱度)

6.0-7.0 (25 °C, 0.5 M in H2O)

溶解性

H2O: 0.5 M at 20 °C, clear, colorless

痕量阴离子

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

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此商品
T12589334893328
assay

≥99.0% (NT)

assay

≥99.0% (titration)

assay

≥99.0% (NT)

assay

≥99.5% (NT)

product line

BioUltra

product line

-

product line

BioXtra

product line

BioUltra

Quality Level

300

Quality Level

300

Quality Level

200

Quality Level

100

form

crystalline powder

form

crystalline powder

form

crystalline powder

form

powder or crystals

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

water: 0.5 M, clear, colorless

solubility

H2O: 0.5 M at 20 °C, clear

solubility

H2O: 0.5 M at 20 °C, clear, colorless

pH

6.0-7.0 (25 °C, 0.5 M in H2O)

pH

6.0-7.0 (0.1 M in water, high purity)

pH

-

pH

3.0-4.5 (25 °C, 0.5 M in H2O)

应用

Trizma被用于配制pH范围在7.5和8.5之间的缓冲液。 Tris缓冲液被广泛用于细胞和分子生物学,如蛋白质和核酸提取和纯化等过程。 Trizma缓冲液也可用于柱层析和凝胶电泳。Trizma acetate用于制备Tris乙酸缓冲液,该缓冲液用作各种测定的稀释剂和电泳运行缓冲液。

其他说明

推荐的缓冲液,用于萤火虫荧光素酶ATP分析的最高灵敏度;谷氨酸结合试验

法律信息

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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    M Ito et al.
    Life sciences, 38(12), 1089-1096 (1986-03-24)
    [3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
    Chi-Lin Li et al.
    The Analyst, 137(22), 5222-5228 (2012-10-04)
    Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation
    Monica Cubillos-Rojas et al.
    Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
    Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
    Patrick S Aranda et al.
    Electrophoresis, 33(2), 366-369 (2012-01-10)
    RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive
    Monica Cubillos-Rojas et al.
    Electrophoresis, 31(8), 1318-1321 (2010-03-24)
    To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a

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