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Merck
CN

93337

Trizma®乙酸盐

BioUltra, ≥99.0% (NT)

别名:

TRIS 乙酸盐, 三(羟甲基)氨基甲烷 乙酸盐

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关于此项目

线性分子式:
NH2C(CH2OH)3 · CH3COOH
化学文摘社编号:
分子量:
181.19
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
EC Number:
229-939-6
Beilstein/REAXYS Number:
3702918
MDL number:
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产品名称

Trizma®乙酸盐, BioUltra, ≥99.0% (NT)

InChI key

PIEPQKCYPFFYMG-UHFFFAOYSA-N

InChI

1S/C4H11NO3.C2H4O2/c5-4(1-6,2-7)3-8;1-2(3)4/h6-8H,1-3,5H2;1H3,(H,3,4)

SMILES string

CC(O)=O.NC(CO)(CO)CO

product line

BioUltra

assay

≥99.0% (NT)

form

crystalline powder

impurities

insoluble matter, passes filter test

ign. residue

≤0.2%

loss

≤0.2% loss on drying, 20 °C (HV)

pH

6.0-7.0 (25 °C, 0.5 M in H2O)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 0.07
λ: 280 nm Amax: 0.05

Quality Level

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Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Trizma被用于配制pH范围在7.5和8.5之间的缓冲液。 Tris缓冲液被广泛用于细胞和分子生物学,如蛋白质和核酸提取和纯化等过程。 Trizma缓冲液也可用于柱层析和凝胶电泳。Trizma acetate用于制备Tris乙酸缓冲液,该缓冲液用作各种测定的稀释剂和电泳运行缓冲液。

Other Notes

推荐的缓冲液,用于萤火虫荧光素酶ATP分析的最高灵敏度;谷氨酸结合试验

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Monica Cubillos-Rojas et al.
Methods in molecular biology (Clifton, N.J.), 869, 205-213 (2012-05-16)
Polyacrylamide gel electrophoresis (PAGE) is one of the most powerful tools used for protein analysis. We describe the use of Tris-acetate buffer and 3-15% polyacrylamide gradient gels to simultaneously separate proteins in the mass range of 10-500 kDa. We show
Monica Cubillos-Rojas et al.
Electrophoresis, 31(8), 1318-1321 (2010-03-24)
To separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3-15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris-acetate buffer (pH 7.0). Samples were prepared in a
Choice of buffer anion for the assay of adenosine 5'-triphosphate using firefly luciferase.
W W Nichols et al.
Analytical biochemistry, 114(2), 396-397 (1981-07-01)
M Ito et al.
Life sciences, 38(12), 1089-1096 (1986-03-24)
[3H]L-glutamic acid binding to microfuge tubes and glass was investigated in four buffers. Background binding to these materials was negligible, but was increased by centrifugation or suction in Tris-HCl and Tris-citrate buffer. This binding was much less or eliminated when
Patrick S Aranda et al.
Electrophoresis, 33(2), 366-369 (2012-01-10)
RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive

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