Merck
CN

C5890

Sigma-Aldrich

酪蛋白 来源于牛奶

suitable for substrate for protein kinase (after dephosphorylation), purified powder

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CAS号:
EC 号:
MDL编号:
NACRES:
NA.61

生物来源

bovine milk

质量水平

形式

purified powder

technique(s)

activity assay: suitable
electrophoresis: suitable
immunocytochemistry: suitable

mp

280 °C (dec.) (lit.)

适用性

suitable for substrate for protein kinase (after dephosphorylation)

UniProt登记号

InChI

1S/C81H125N22O39P/c1-36(2)31-50(76(132)94-43(15-24-57(87)108)71(127)101-52(34-64(120)121)78(134)98-49(81(137)138)11-7-8-30-82)99-72(128)47(19-28-61(114)115)95-77(133)51(33-63(118)119)100-73(129)48(20-29-62(116)117)97-80(136)65(37(3)104)103-75(131)44(16-25-58(88)109)92-68(124)42(14-23-56(86)107)90-67(123)41(13-22-55(85)106)91-69(125)45(17-26-59(110)111)93-70(126)46(18-27-60(112)113)96-79(135)53(35-142-143(139,140)141)102-74(130)40(12-21-54(84)105)89-66(122)39(83)32-38-9-5-4-6-10-38/h4-6,9-10,36-37,39-53,65,104H,7-8,11-35,82-83H2,1-3H3,(H2,84,105)(H2,85,106)(H2,86,107)(H2,87,108)(H2,88,109)(H,89,122)(H,90,123)(H,91,125)(H,92,124)(H,93,126)(H,94,132)(H,95,133)(H,96,135)(H,97,136)(H,98,134)(H,99,128)(H,100,129)(H,101,127)(H,102,130)(H,103,131)(H,110,111)(H,112,113)(H,114,115)(H,116,117)(H,118,119)(H,120,121)(H,137,138)(H2,139,140,141)

InChI key

BECPQYXYKAMYBN-UHFFFAOYSA-N

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一般描述

来自牛奶的酪蛋白是一种磷蛋白。酪蛋白有四种主要类型,占牛奶中总蛋白质的约80%:α-s1 酪蛋白、α-s2 酪蛋白、β-酪蛋白和κ-酪蛋白。酪蛋白被认为是牛奶中的主要保护成分。

应用

源自牛乳的酪蛋白用于:
  • 制备酪蛋白水解物
  • 作为双缩脲法中的校准物质
  • 作为营养琼脂的补充剂,用于测定蛋白酶活性
  • 为果蝇( D. melanogaster)的独立复制品系准备富含蛋白的饲料

生化/生理作用

部分消化的酪蛋白是生物活性肽的丰富来源,如β酪啡肽。然而,牛酪蛋白是不同质的;A1和B变异的确会导致产生β-酪啡肽7,但A2却不会。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, type N95 (US)


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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. What is the composition of Product C5890, Casein from bovine milk?

    The approximate casein composition of milk in grams per Liter is: alpha-s1 (12-15); alpha-s2 (3-4); beta (9-11); and kappa (2-4).

  7. What is the molecular weight of the casein subunits in Product C5890, Casein from bovine milk?

    The alpha-s1 = 22-23.7 kDa, alpha-s2 = 25 kDa, beta = 24 kDa and kappa = 19 kDa.

  8. What is Product C5890, Casein from bovine milk, soluble in?

    Casein is not water soluble. It can be suspended in 1 M sodium hydroxide at a concentration of 50 mg/mL, yielding a turbid, faint yellow solution.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

N D Richert et al.
The Journal of biological chemistry, 257(12), 7143-7150 (1982-06-25)
We recently described a method for the purification of a protein kinase related to pp60src from Rous sarcoma virus-induced rat tumors (Blithe, D. L., Richert, N. D., and Pastan, I. H. (1982) J. Biol. Chem. 257, 7135-7142). In this report
Dietary protein content affects evolution for body size, body fat and viability in Drosophila melanogaster
Kristensen TN, et al.
Biology Letters, 7(2), 269-272 (2010)
Assay of cyclic AMP by protein kinase activation.
S E Mayer et al.
Methods in enzymology, 38, 66-73 (1974-01-01)
Guo-Yue Wan et al.
Archives of medical science : AMS, 18(2), 376-381 (2022-03-24)
More than half of the world's population is infected with Helicobacter pylori, which may cause gastritis, peptic ulcer and even gastric cancer. The World Health Organization (WHO) has announced that H. pylori infection is a class I carcinogen and hence
Esterase and protease activities of Bacillus spp. from afitin, iru and sonru; three African locust bean (Parkia biglobosa) condiments from Benin
Azokpota P, et al.
African Journal of Biotechnology, 5(3), 265-272 (2006)

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