biological source
rat brain
packaging
tube of 5 μg 00031626-DNA-5UG, pkg of vial of cells 00031626-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Endothelial
products
Not specified
receptors
Vasopressin V1a, Angiotensin II, Endothelin 1, Bradykinin, Serotonin
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Toxicology, cellular signalling and pharmacology studies
Biochem/physiol Actions
Rat Choroid Plexus
The choroid plexus of adult Wistar Rats were dissected from the fourth ventricle to give a primary culture. This primary culture was then immortalized by transfection with the TSori minus adenovirus (a replication deficient adenovirus containing SV40 large T antigen). Sub-cloning of the immortalised cells was performed by selecting for clones which exhibited calcium ion release in response to vasopressin, angiotensin, bradykinin, serotonin or endothelin stimulation. The sub-clone which exhibited the largest response to all five hormones was selected and sub-cultured on to become RCP.
Preparation Note
DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 0.1% Epidermal Growth Factor(EGF) (E9644), 1% Insulin–Transferrin–Selenium(ITS) (I3146)
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C
Other Notes
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