B12.F8

Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing.CM3.B6 (Sigma product number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.

Human anti-hepatitis C virus IgG1 secreting B cell

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16

RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interleukin-6 (IL-6).

Maintain cultures between 3-9 x 10⁵/ml. 5% CO₂; 37 °C. Media change every four days. Saturation density 1.1 x 10⁶/ml.

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This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.