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Merck
CN

B12.F8

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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UNSPSC代码:
41106514
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生物来源

human blood

包装

tube of 5 μg 11060102-DNA-5UG
pkg of vial of cells 11060102-1VL

生长模式

Suspension

核型

Not specified

形态学

Small rounded cells

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

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生化/生理作用

Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. This antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. This antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing.CM3.B6 (Sigma product number 11060101) and B12.F8 were derived from the same patient as confirmed by STR profiling.
Human anti-hepatitis C virus IgG1 secreting B cell
STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
THO1: 6
TPOX: 9,11
vWA: 14,16

制备说明

RPMI 1640 (R0883) + 2 mM L-L-Glutamine (G7513) + 1% NEAA (M7145) + 1% NaP (S8636) + 10% FBS / FCS (F2442) + 20U/ml Interleukin-6 (IL-6).
Maintain cultures between 3-9 x 105/ml. 5% CO2; 37 °C. Media change every four days. Saturation density 1.1 x 106/ml.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.

法规信息

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Lot/Batch Number

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