CHO-K1.Cl-0270

Clone of CHO-K1 cells expressing * Macaca mulatta (Rhesus macaque) / * Macaca fascicularis (Cynomolgus macaque) FcγRIIb as a transmembrane protein.
* The sequence of the extracellular domains of FcγRIIb from Rhesus macaque (NP_001258577) and a predicted sequence for the one polymorphic form of FcγRIIb from Cynomolgus macaque (NW_005092960) are identical at the amino acid level. There is one amino acid change (Leu104Met) relative to the Cynomolgus macaque sequence AAL92097

Stably transfected with a pcDNA3.1/Hygro(+) based vector with a cDNA insert that had been modified to enable expression of Rhesus macaque / Cynomolgus macaque FcγRIIb as a transmembrane receptor

MEM (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 10% FBS (F2442) with 600ug/ml hygromycin B

Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO₂/95%air; 37^oC

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.Cl-0270 (ECACC 15042912)

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.