grade
Molecular Biology
Quality Level
description
recommended for cultivation of recombinant E. coli.
sterility
non-sterile
form
powder
composition
KCl, 0.186 g/L , MgSO4, 2.4 g/L , NaCl, 0.5 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2.8% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
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General description
Hannahan’s 肉汤 (SOB)是用于制备和转化感受态细胞（大肠杆菌）的微生物生长培养基。 酪蛋白酶解产物和酵母提取物为重组 大肠杆菌提供含氮化合物和生长因子。氯化钾和氯化钠可维持细胞的等渗环境。培养基中加入硫酸镁，作为 DNA 复制的必要成分。
Application
建议将Hanahan′s Broth用于大肠杆菌的重组菌株的培养。它适用于制备感受态 大肠杆菌，通常添加0.4％的葡萄糖。它还可用于制备 钩虫贪铜菌 H16 感受态细胞。它适用于 大肠杆菌 菌株的非选择性培养，用于克隆、生产DNA质粒和生产重组蛋白。
Features and Benefits
Hannahan′s Broth（SOB）粉末提供：
- 标准配方
- 液体形式的廉价替代品
Preparation Note
通过搅拌使28g粉末悬浮在1L水中。在121℃下高压灭菌15分钟。在加入添加物，例如抗生素（如果需要），之前请先冷却。
Other Notes
酪蛋白酶酶解产物和酵母抽提物提供了必要的氮化合物和生长因子。 氯化钾和氯化钠为细胞提供了等渗环境。 硫酸镁有助于DNA复制。
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
法规信息
监管及禁止进口产品
此项目有
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 1-1 (1989)
The genetic basis of 3-hydroxypropanoate metabolism in Cupriavidus necator H16
Arenas-LC, et al.
Biotechnology for Biofuels, 12, 150-150 (2019)
Difco Manual, 444-444 (1998)
Nicole Pearcy et al.
PLoS computational biology, 18(5), e1010106-e1010106 (2022-05-24)
Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly promising microbial chassis due to its ability to
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
相关内容
Datasheet
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H8032-500G | 04061833805008 |
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