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Merck
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主要文件

L2542

Sigma-Aldrich

LB 肉汤

Liquid microbial growth medium

别名:

Luria Broth Miller, Luria-Bertani broth, Miller

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About This Item

UNSPSC代码:
41106200
NACRES:
NA.85

等级

Molecular Biology

质量水平

无菌性

sterile; 0.2 μm filtered

表单

liquid

技术

microbiological culture: suitable

pH值(酸碱度)

6.5-7.2

应用

microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

一般描述

Miller’s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. It enables fast growth, improves growth yields for various bacterial species, and can retain the hydrophilic behavior of samples for longer periods.

应用

LBBroth (Miller) is suitable for:

  • non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
  • selective cultivation when appropriate antibiotics are added.
  • the cultivation of E-coli cells for anti-bacterial assays.

特点和优势

Miller′s LB liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

组分

10g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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