grade
Molecular Biology
Quality Level
sterility
non-sterile
form
powder
composition
NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(2% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
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General description
Lennox LB是一个被高度引用的用于 大肠杆菌培养的微生物生长培养基。这种富含营养的微生物肉汤含有肽、氨基酸和低盐配方的碳水化合物。
Application
Luria肉汤被用于大肠杆菌（Escherichia coli）的维持和繁殖。 大肠杆菌在Luria肉汤中生长得更快，因为胰蛋白胨和酵母为其提供了必需的生长因子，从而无需大肠杆菌自身合成。由于含有NaCl成分，Luria肉汤还含有转运和渗透平衡所必需的电解质。
在三鞭毛虫细胞提取物的分级研究中，它被用于梨形四膜虫胞级分的重悬。
在三鞭毛虫细胞提取物的分级研究中，它被用于梨形四膜虫胞级分的重悬。
适用于 大肠杆菌 菌株的非选择性培养，用于克隆、生产DNA质粒和生产重组蛋白。 也适用于添加适当抗生素时的选择性培养，包括需要低盐条件的抗生素，如吉欧霉素®。
Features and Benefits
Lennox LB粉末可提供：
- 便捷式小包装，无需称重步骤
- 使用大包装可轻松放大规模
- 一种预算较低的液体替代品
- 标准配方
Preparation Note
1.将20 g悬浮于1L蒸馏水中。
2.121 °C下高压灭菌15分钟。
制备LB肉汤：添加1 g葡萄糖，然后按照上述说明操作。
制备Enquist &Sternberg培养基：高压蒸汽灭菌后，以无菌操作加入10 mL无菌1M硫酸镁。
2.121 °C下高压灭菌15分钟。
制备LB肉汤：添加1 g葡萄糖，然后按照上述说明操作。
制备Enquist &Sternberg培养基：高压蒸汽灭菌后，以无菌操作加入10 mL无菌1M硫酸镁。
Legal Information
Zeocin is a registered trademark of Cayla Sarl
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
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实验方案
General protocols for growth of competent cells in microbial medium.
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| L3022-6X1KG | 04061835573448 |
| L3022-1KG | 04061835573424 |
| L3022-250G | 04061835573431 |
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