等级
Molecular Biology
质量水平
无菌性
non-sterile
表单
powder
组成
Casamino Acids, 1 g/L
MgSO4, 0.98 g/L
NaCL, 5 g/L
Tryptone, 10 g/L
Yeast extract, 5 g/L
技术
microbiological culture: suitable
pH值(酸碱度)
6.8-7.2(2.2% solution)
应用
food and beverages
microbiology
储存温度
room temp
适用性
nonselective for Escherichia coli
nonselective for coliforms
一般描述
NZCYM 是用于培养 大肠杆菌 和 λ 噬菌体的微生物生长培养基。这种营养丰富的微生物肉汤含有多肽、氨基酸和水溶性维生素,呈低盐配方。
应用
NZCYM 肉汤可用于非选择性培养lambda, MS2, T7, CLP-P3, Phi29, 和 Goe1 噬菌体, 以及用于克隆,DNA质粒生产和重组蛋白生产的 大肠杆菌 菌株。 当加入适当抗生素时,也适用于选择性培养。
特点和优势
NZCYM 粉末:
- 标准配方
- 方便的包装规格
- 一种预算较低的液体替代品
制备说明
通过搅拌使22 g粉末悬浮在1L水中。在121℃下高压灭菌15分钟。在加入添加物,例如抗生素(如果需要),之前请先冷却。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Shane R Wilkinson et al.
The Journal of biological chemistry, 277(19), 17062-17071 (2002-02-14)
Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show
Cell-free production of personalized therapeutic phages targeting multidrug-resistant bacteria
Emslander Q.
Cell Chemical Biology (2022)
Bioreagents for Cloning
Sigma data, 2-2 (2011)
Chris Coward et al.
Applied and environmental microbiology, 72(7), 4638-4647 (2006-07-06)
This study characterizes the interaction between Campylobacter jejuni and the 16 phages used in the United Kingdom typing scheme by screening spontaneous mutants of the phage-type strains and transposon mutants of the sequenced strain NCTC 11168. We show that the
Carla M Carvalho et al.
BMC microbiology, 10, 232-232 (2010-09-03)
Poultry meat is one of the most important sources of human campylobacteriosis, an acute bacterial enteritis which is a major problem worldwide. Campylobacter coli and Campylobacter jejuni are the most common Campylobacter species associated with this disease. These pathogens live
实验方案
在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。
General protocols for growth of competent cells in microbial medium.
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