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S2076

Sigma-Aldrich

α-2,6-唾液酸转移酶 来源于美人鱼发光杆菌

recombinant, expressed in E. coli BL21, ≥5 units/mg protein

All Photos(2)
Synonym(s):
β-半乳糖苷-α-2,6-唾液酸转移酶, CMP-N-乙酰神经氨酸酯：β-D-半乳糖基-1,4-N-乙酰基-β-D-葡糖胺-α-2,6-N-乙酰神经氨酸转移酶
Enzyme Commission number:
2.4.99.1 (BRENDA, IUBMB)
MDL编号:
MFCD00132245

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重组

expressed in E. coli BL21

质量水平

200

形式

lyophilized powder

specific activity

≥5 units/mg protein

分子量

56.8 kDa

运输

dry ice

储存温度

−20°C

一般描述

人ST6Gal-1（β半乳糖苷α-2,6-唾液酸转移酶1）是CAZy家族GT29的成员。

应用

α来自美人鱼发光杆菌（Photobacterium damsela） 的-2,6-唾液酸转移酶已被用于HRT-18G细胞中唾液酸（SA）的再唾液酸化（resialylation）和恢复。
高活性α 2-6唾液酸转移酶已用于制备高级别的的二唾液酸化片段晶体。

包装

1 unit

生化/生理作用

α复杂的N-聚糖生物合成的最终步骤是由-2,6-唾液酸转移酶（ST）催化。与真核生物α（2,6）-ST相比，细菌α（2,6）-ST具有更广泛的受体底物特异性。
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac转移至受体分子（包括糖蛋白、糖脂和寡糖）的半乳糖基末端。

单位定义

在37℃，pH8.0条件下，一个单位每分钟可催化由CMP-Neu-5-Ac和Lac-β-OMU形成1 μmol Neu-5-Ac-α -2,6-LacMU。

外形

以含有Tris-HCl和NaCl的冻干粉末形式提供。

分析说明

酶活性测定在含有CMP-Neu-5-Ac（1 mM）和Lac-β−OMU（1 mM）的Tris-HCl缓冲液（100 mM，pH 8.0）中于37°C进行30分钟，并使用带有荧光检测器（激发波长325nm，发射波长372nm）的HPLC进行分析。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Regulatory Information

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Certificate of Analysis

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Certificate of Origin

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