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Merck
CN

S2076

α-2,6-唾液酸转移酶 来源于美人鱼发光杆菌

recombinant, expressed in E. coli BL21, ≥5 units/mg protein

别名:

β-半乳糖苷-α-2,6-唾液酸转移酶, CMP-N-乙酰神经氨酸酯：β-D-半乳糖基-1,4-N-乙酰基-β-D-葡糖胺-α-2,6-N-乙酰神经氨酸转移酶

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关于此项目

UNSPSC Code:
12352204
NACRES:
NA.54
EC 号:
2.4.99.1 (BRENDA, IUBMB)
MDL number:
MFCD00132245

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产品名称

α-2,6-唾液酸转移酶 来源于美人鱼发光杆菌, recombinant, expressed in E. coli BL21, ≥5 units/mg protein

recombinant

expressed in E. coli BL21

form

lyophilized powder

specific activity

≥5 units/mg protein

mol wt

56.8 kDa

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

相关类别

Other Notes

在37℃，pH8.0条件下，一个单位每分钟可催化由CMP-Neu-5-Ac和Lac-β-OMU形成1 μmol Neu-5-Ac-α -2,6-LacMU。

Analysis Note

酶活性测定在含有CMP-Neu-5-Ac（1 mM）和Lac-β−OMU（1 mM）的Tris-HCl缓冲液（100 mM，pH 8.0）中于37°C进行30分钟，并使用带有荧光检测器（激发波长325nm，发射波长372nm）的HPLC进行分析。

Application

α来自美人鱼发光杆菌（Photobacterium damsela） 的-2,6-唾液酸转移酶已被用于HRT-18G细胞中唾液酸（SA）的再唾液酸化（resialylation）和恢复。
高活性α 2-6唾液酸转移酶已用于制备高级别的的二唾液酸化片段晶体。

Biochem/physiol Actions

α复杂的N-聚糖生物合成的最终步骤是由-2,6-唾液酸转移酶（ST）催化。与真核生物α（2,6）-ST相比，细菌α（2,6）-ST具有更广泛的受体底物特异性。
唾液酸转移酶可将Neu5Ac从CMP-Neu5Ac转移至受体分子（包括糖蛋白、糖脂和寡糖）的半乳糖基末端。

General description

人ST6Gal-1（β半乳糖苷α-2,6-唾液酸转移酶1）是CAZy家族GT29的成员。

Physical form

以含有Tris-HCl和NaCl的冻干粉末形式提供。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000415709
0000415709
0000356578
0000318578
0000356578

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Masayoshi Onitsuka et al.
Applied microbiology and biotechnology, 94(1), 69-80 (2011-12-30)
Improvement of glycosylation is one of the most important topics in the industrial production of therapeutic antibodies. We have focused on terminal sialylation with alpha-2,6 linkage, which is crucial for anti-inflammatory activity. In the present study, we have successfully cloned
Chompunuch Boonarkart et al.
Journal of medical virology, 84(3), 380-385 (2012-01-17)
A case of unusually high severity of influenza pneumonia leading to acute respiratory distress syndrome and death was investigated. This was a previously a healthy 28-year-old man with no underlying conditions, admitted to a hospital during the first wave of
N Li et al.
Acta virologica, 55(2), 147-153 (2011-06-23)
Human influenza viruses are major concern as the leading cause of global pandemics. In infecting cells, they preferentially bind to sialyloligosaccharides containing terminal N-acetyl sialic acid linked to galactose by an α-2,6-linkage (NeuAcα2,6Gal). The amount of NeuAcα2,6Gal in Vero cells
Canine Respiratory Coronavirus, Bovine Coronavirus, and Human Coronavirus OC43: Receptors and Attachment Factors
Szczepanski A, et al.
Viruses, 11(4), 328-328 (2019)
Adam W Barb et al.
Biochemistry, 51(22), 4618-4626 (2012-05-12)
The terminal carbohydrate residues of the N-glycan on the immunoglobulin G (IgG) fragment crystallizable (Fc) determine whether IgG activates pro- or anti-inflammatory receptors. The IgG Fc alone becomes potently anti-inflammatory upon addition of α2-6-linked N-acetylneuraminic acid residues to the N-glycan
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