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CN

Y0626

不含氨基酸的酵母氮源基

Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

别名:

ynb without amino acids

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NACRES:
NA.85
UNSPSC Code:
41106212

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grade

Molecular Biology

sterility

non-sterile

form

powder

technique(s)

microbe id | utilization test: suitable, microbiological culture: suitable

pH

5.2-5.6(0.67% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp., yeasts

Quality Level

200

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General description

酵母氮基是培养野生型酵母公认的理想选择。目前可确知，氨基酸存在会妨碍选择性标记物使用。因此，这种培养基制剂之中不含氨基酸，可用于酵母人工染色体的克隆和操作。
酵母菌氮基是酵母菌生长的重要培养基。 这种富含营养的微生物肉汤含有氮、维生素、微量元素和盐。

Application

不含氨基酸的酵母氮基已用于：
  • 合成脂解耶氏酵母培养和鉴定所用的完全补充混合物 (SC)-Leu培养基
  • 用作酵母尿嘧啶营养性生长的选择性液体培养基组分
  • 评估酿酒酵母菌株在不同的营养条件下的转化效率

适用于根据氨基酸和碳的需要对酵母菌进行分类。 可能需要添加氨基酸和碳源(通常是葡萄糖)。

Preparation Note

1.将6.7 g酵母氮碱、5 g葡萄糖或等量的其他碳水化合物和5-10 mg所需氨基酸溶解在100 ml蒸馏水中，制备10x储备溶液。
2.必要时可加温溶解，然后过滤灭菌。
2-8°C储存10x储备溶液。使用前，在无菌条件下用无菌蒸馏水1:10稀释。

Other Notes

组成部分，含量(μg/L，除非注明)

氮源：
硫酸铵，5.0 g/L

维生素：
生物素，2.0
泛酸钙，400
叶酸，2.0
肌醇，2.0 mg / L
烟酸，400
对氨基苯甲酸，200
盐酸吡哆醇，400
核黄素，200
盐酸硫胺素，400

微量元素：
硼酸，500
硫酸铜，40
碘化钾，100
氯化铁，200
硫酸锰，400
钼酸钠，200
硫酸锌，400

盐类：
磷酸二氢钾，1.0g/L
硫酸镁，0.5g/L
氯化钠，0.1g/L
氯化钙，0.1g/L

存储类别

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCZ7887
MKCZ1703
MKCX8697
MKCX2984
MKCW6581

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Journal of applied microbiology, 127(3), 845-855 (2019-06-19)
The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial
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R Daniel Gietz et al.
Nature protocols, 2(1), 38-41 (2007-04-03)
Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes
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酵母生长实验方案

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。

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Product Information Sheet - Y0626

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