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安全信息

Y0626

Sigma-Aldrich

不含氨基酸的酵母氮源基

Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

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别名:
ynb without amino acids
NACRES:
NA.85

等级

for molecular biology

质量水平

200

无菌性

non-sterile

形式

powder

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

pH值（酸碱度）

5.2-5.6(0.67% solution)

application(s)

food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts

相关类别

一般描述

酵母氮基是培养野生型酵母公认的理想选择。目前可确知，氨基酸存在会妨碍选择性标记物使用。因此，这种培养基制剂之中不含氨基酸，可用于酵母人工染色体的克隆和操作。
酵母菌氮基是酵母菌生长的重要培养基。 这种富含营养的微生物肉汤含有氮、维生素、微量元素和盐。

应用

不含氨基酸的酵母氮基已用于：
  • 合成脂解耶氏酵母培养和鉴定所用的完全补充混合物 (SC)-Leu培养基
  • 用作酵母尿嘧啶营养性生长的选择性液体培养基组分
  • 评估酿酒酵母菌株在不同的营养条件下的转化效率

适用于根据氨基酸和碳的需要对酵母菌进行分类。 可能需要添加氨基酸和碳源(通常是葡萄糖)。

组分

组成部分，含量(μg/L，除非注明)

氮源：
硫酸铵，5.0 g/L

维生素：
生物素，2.0
泛酸钙，400
叶酸，2.0
肌醇，2.0 mg / L
烟酸，400
对氨基苯甲酸，200
盐酸吡哆醇，400
核黄素，200
盐酸硫胺素，400

微量元素：
硼酸，500
硫酸铜，40
碘化钾，100
氯化铁，200
硫酸锰，400
钼酸钠，200
硫酸锌，400

盐类：
磷酸二氢钾，1.0g/L
硫酸镁，0.5g/L
氯化钠，0.1g/L
氯化钙，0.1g/L

制备说明

1.将6.7 g酵母氮碱、5 g葡萄糖或等量的其他碳水化合物和5-10 mg所需氨基酸溶解在100 ml蒸馏水中，制备10x储备溶液。
2.必要时可加温溶解，然后过滤灭菌。
2-8°C储存10x储备溶液。使用前，在无菌条件下用无菌蒸馏水1:10稀释。

相关产品

产品编号
说明
价格

G7021

D -(+)-葡萄糖, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A5306

细菌琼脂, for molecular biology

Y2001

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan and uracil

Y1876

酵母营养缺陷型培养基补充剂, without tryptophan

Y1250

酵母含氨基酸的氮碱, Non-selective yeast growth medium

Y1771

酵母营养缺陷型培养基补充剂, without uracil, leucine, and tryptophan

Y1896

酵母营养缺陷型培养基补充剂, without lysine

Y2021

酵母营养缺陷型培养基补充剂, without histidine, leucine, tryptophan, and adenine

Y2146

酵母营养缺陷型培养基补充剂, without histidine, leucine, and tryptophan

B11001

EnPresso® B, Growth system for expressing protein in bacteria

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基

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The PH gene determines fruit acidity and contributes to the evolution of sweet melons
Cohen S, et al.
Nature Communications, 5, 4026-4026 (2014)
Ying Liu et al.
Biological procedures online, 13, 6-6 (2011-08-17)
Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media
Liangping Li et al.
Nature protocols, 8(8), 1567-1582 (2013-07-23)
Introducing human genes into mice offers the opportunity to analyze their in vivo function or to obtain therapeutic molecules. For proper gene regulation, or in case of multigene families, megabase (Mb)-sized DNA fragments often have to be used. Yeast artificial
Peter Hayek et al.
Microbiological research, 165(3), 250-258 (2009-07-21)
Various factors are thought to be responsible for Candida albicans virulence, such as lipases, proteases and adhesins. Many of these factors are GPI-anchored cell surface proteins responsible for pathogenicity. Hwp2 is a putative GPI-anchored protein. The purpose of this study
Patrick F Suthers et al.
Metabolic engineering communications, 11, e00148-e00148 (2020-11-03)
Many platform chemicals can be produced from renewable biomass by microorganisms, with organic acids making up a large fraction. Intolerance to the resulting low pH growth conditions, however, remains a challenge for the industrial production of organic acids by microorganisms.
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酵母生长实验方案

酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。

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