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应用
Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.
由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。
其他说明
氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
制备说明
Dissolve 1.46 g of supplement per liter of medium.
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产品编号
说明
价格
警示用语:
Danger
危险声明
危险分类
Eye Dam. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.
实验方案
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
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