Adjust times as necessary for the specific gene
target studied.
Cat. #64-101
siIMPORTER™ Transfection Reagent
18
C. Plasmid DNA Protocol
Transfection of Adherent Cells:
1. Plate cells
sealed at 2–8 °C.
Reagent preparation
Prepare 1x Wash buffer by adding the contents of the
bottle (25 mL, 20x) to 475 mL of distilled or deionized
water. Store at room temperature (18–26 °C).
Storage
temperature (18–26 °C) and gently mix.
1. Place the desired number of coated strips into the
holder.
2. Negative control, positive control, and calibrator are
ready to use. Prepare 101-fold dilution
temperature (18–26 °C) and gently mix.
1. Place the desired number of coated strips into the
holder.
2. Negative control, positive control, and calibrator are
ready to use. Prepare 101-fold dilution
ZipTip® with 0.2 µL C 18 resin, 960/pk ZTC18M960
ZipTip® with 0.6 µL C 18 resin, 8/pk ZTC18S008
ZipTip® with 0.6 µL C 18 resin, 96/pk ZTC18S096
ZipTip® with 0.6 µL C 18 resin, 960/pk ZTC18S960
HYDROCHLORIDE
Methanol Solution
Product Number A 8535
Lot Number 42K8801
CAS Number [549-18-8]
Concentration 1.12 mg Amitriptyline Hydrochloride
(equivalent to 0.99 mg free base)/mL
GC grade
Catalog # SCYT1-91K, SCYT-91K-PX14, and SCYT-91K-PXBK14
Control Catalog # SCYT1-6091 Lot# SCYT1-101 and SCYT1-201
Note: The Quality Control Ranges are generated using serum matrix provided in the
ZipTip® with 0.2 µL C 18 resin, 960/pk ZTC18M960
ZipTip® with 0.6 µL C 18 resin, 8/pk ZTC18S008
ZipTip® with 0.6 µL C 18 resin, 96/pk ZTC18S096
ZipTip® with 0.6 µL C 18 resin, 960/pk ZTC18S960
products.
This culture medium complies with the specifications given by EN ISO 4833, ISO 6730 I
IDF 101, ISO 8552 I IDF 132, ISO 17410 and APHA (SMA with skimmed milk: Standard
Methods Agar with skimmed
Salmosyst ® zur Anreicherung von
Salmonellen aus Kotproben von Tieren. - Berl. Münch. Tierärztl. Wschr.,
101; 57-59 (1988).
OSSMER, R.: Salmosyst® and RAMBACH-Agar. A Rapid Alternative for the
Detection of Salmonella
P et al (1985). The Complement of Desmosomal Plaque Proteins in Different Cell
Types. J Cell Biol 101: 1442-1454.
Franke, W. W. et al. (1983). Proc. Nat. Acad. Sci. 80: 543-47.
Important Note: During