of Human OPCs (24 well plate)
Note: For qRT-PCR experiments, we recommend culturing the cells in T25 flasks instead of 24-well
plates. Plating density for T25 flasks should be 2 x 104 cells/cm2, which
of Human OPCs (24 well plate)
Note: For qRT-PCR experiments, we recommend culturing the cells in T25 flasks instead of 24-well
plates. Plating density for T25 flasks should be 2 x 104 cells/cm2, which
of Human OPCs (24 well plate)
Note: For qRT-PCR experiments, we recommend culturing the cells in T25 flasks instead of 24-well
plates. Plating density for T25 flasks should be 2 x 104 cells/cm2, which
Tartrate Acid Buffer, Catalog No.104-12
Tube 2: 0.5 ml Citrate Buffer, Catalog No.104-4
Tube 3: 0.5 ml Citrate Buffer, Catalog No.104-4
Place tubes in 37°C water bath for about 5 minutes.
3. To Tubes 1 and
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
Tartrate Acid Buffer, Catalog No.104-12
Tube 2: 0.5 ml Citrate Buffer, Catalog No.104-4
Tube 3: 0.5 ml Citrate Buffer, Catalog No.104-4
Place tubes in 37°C water bath for about 5 minutes.
3. To Tubes 1 and
Tartrate Acid Buffer, Catalog No.104-12
Tube 2: 0.5 ml Citrate Buffer, Catalog No.104-4
Tube 3: 0.5 ml Citrate Buffer, Catalog No.104-4
Place tubes in 37°C water bath for about 5 minutes.
3. To Tubes 1 and
Tartrate Acid Buffer, Catalog No.104-12
Tube 2: 0.5 ml Citrate Buffer, Catalog No.104-4
Tube 3: 0.5 ml Citrate Buffer, Catalog No.104-4
Place tubes in 37°C water bath for about 5 minutes.
3. To Tubes 1 and
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
1.
Example of neuron morphology at different time points
post-seeding. Neurons were seeded at 5 × 104 live
cells/cm2.
Figure 2.
Example of mature neurons. Immunostaining at
10 days post-seeding
dish 78.5 3,000 2 10
60 mm dish 21.29 1,500 0.5 5
1 well of a 6 well plate 9.62 750 0.25 3–4
1 well of a 12 well plate 3.80 300 0.1 2
1 well of a 24 well plate 2.00
dish 78.5 3,000 2 10
60 mm dish 21.29 1,500 0.5 5
1 well of a 6 well plate 9.62 750 0.25 3–4
1 well of a 12 well plate 3.80 300 0.1 2
1 well of a 24 well plate 2.00