CN
105-90-8
应用筛选条件
关键词:'105-90-8'
显示 1-30 共 470 条结果 关于 "105-90-8" 范围 技术文档
The Effective Use of Protein Kinase Inhibitors
1 95 59 6 72 36 93 84 94 14 26 90 101 82 61
48 75 38 99 86 8 6 54 66 10 83 105 106 103
99 98 103 104 101 67 95 77 101
The Effective Use of Protein Kinase Inhibitors
1 95 59 6 72 36 93 84 94 14 26 90 101 82 61
48 75 38 99 86 8 6 54 66 10 83 105 106 103
99 98 103 104 101 67 95 77 101
EX-CELL 302 Serum-Free Medium for CHO Cells
of 2 x 105 cells/mL.
7. Observe cells daily. Gently rap the flask against the palm
of the hand to detach cells, return to the incubator until
cells reach 80 - 90% confluency (~ 4 - 5 days).
8. Once
EX-CELL 302 Serum-Free Medium for CHO Cells
of 2 x 105 cells/mL.
7. Observe cells daily. Gently rap the flask against the palm
of the hand to detach cells, return to the incubator until
cells reach 80 - 90% confluency (~ 4 - 5 days).
8. Once
Product Information Sheet - 14326C
2 x 105 cells/mL.
7. Observe cells daily. Gently rap the flask against
the palm of the hand to detach cells, return to the
incubator until cells reach 80 - 90% confluency
(~ 4 - 5 days).
Product Information Sheet - 14324C
2 x 105 cells/mL.
7. Observe cells daily. Gently rap the flask against
the palm of the hand to detach cells, return to the
incubator until cells reach 80 - 90% confluency
(~ 4 - 5 days).
Evaluation of MDCK Cell Growth and Virus Production in EX-CELL MDCK
using a cryopreservation medium
consisting of 90% fresh EX-CELL MDCK with 10% DMSO
(referred to as “90:10”). Three 175 cm2 T-fl asks were seeded
with 1 x 105 cells/cm2 in EX-CELL MDCK. After 3 days, the
Product Information Sheet - M6395
MCDB 105 MEDIUM (M6395) - Product Information Sheet
MCDB 105 MEDIUM
With L-Glutamine and 25 mM HEPES
Product Number M6395
Storage Temperature 2-8°C
Product Description
MCDB media were designed
EX-CELL CD CHO Serum-Free Medium for CHO Cells
CHO medium
supplemented with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
EX-CELL CD CHO Serum-Free Medium - 24360C
CHO medium
supplemented with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
EX-CELL CD CHO Serum-Free Medium - 14361C
CHO medium
supplemented with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
EX-CELL CD CHO Serum-Free Medium - 14360C
CHO medium
supplemented with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
EX-CELL NS0 Serum-Free Medium for NS0 Cells Chemically Defined
seeding density of
|2 x 105 cells/mL in shaker flasks.
2. Incubate the flasks at 37 C in a humidified incubator with
5% CO2. Maintain the orbital shaker speed between
105 - 115 rpm.
3. Continue
EX-CELL NS0 Serum-Free Medium for NS0 Cells Chemically Defined
serum-supplemented medium
into EX-CELLTM NS0 (supplemented with 8 mM L-glutamine
as described previously) at a minimum seeding density of
2 x 105 cells/mL in shaker flasks.
2. Incubate the flasks at 37 C
Verfication summary-03-0202-00
336.8 12% 90%
3 1:32 92.14 3.6 4% 2948.33 115.0 4% 91%
HMN627647
3 MRD (1:4) 31.56 2.8 9% 126.24 11.4 9% -
3 1:8 17.72 1.3 7% 141.78 10.1 7% 112%
3 1:16 8.32 0.6
Verfication summary-03-0202-00
336.8 12% 90%
3 1:32 92.14 3.6 4% 2948.33 115.0 4% 91%
HMN627647
3 MRD (1:4) 31.56 2.8 9% 126.24 11.4 9% -
3 1:8 17.72 1.3 7% 141.78 10.1 7% 112%
3 1:16 8.32 0.6
Process Guidance - Cellvento BHK-200
40
20
0
100
95
90
85
80
V
ia
bi
lit
y
(%
)
0 8 16 24 32 40 48 56 64 72 80 88
GMEM Cellvento®
EX-CELL CHO Medium (C4726)
viability is at least
90% and the cells are in the mid-logarithmic growth
phase. Cells grown in serum-containing medium
should be inoculated at a viable cell density of 2 x 105
cells/ml in a 1:1 mixture
Data Sheet - G9916
For maintaining cultures in Gene Therapy
Medium-3, seed stock cultures at 2 x 105
cells/ml for three days or at 3 x 105 cells/ml for
two days.
NOTE: This maintenance schedule is appropriate
Data Sheet - G0916
For maintaining cultures in Gene Therapy
Medium-1, seed stock cultures at 2 x 105
cells/ml for three days or at 3 x 105 cells/ml for
two days. NOTE: This maintenance schedule is
appropriate for both
Product Information Sheet - VP002
Cells were seeded in the 3 L bioreactor at
6 × 105 viable cells (vc) per mL. Peak viable cell density (VCD) of 12 × 106 vc/mL was obtained
with viability >90%.
P0
Application Note - Universal ProbeLibrary Set: One Transcriptome - One Kit
optimize a single,
poorly performing assay – can save time and money.
M 106 105 104 103 102 10 1
HybProbe ProbeLibrary
M 106 105 104 103 102 10 1 M
b2M copy number
M
b2M copy
Product Information -24370C
Viability should be higher
than 90% at all times.
3. Determine the correct volume of cell culture to
inoculate a new flask at a starting cell density of
2–3×105 viable cells/mL for the targeted
Data Sheet - G0791
For maintaining cultures in Gene Therapy Medium-2,
seed stock cultures at 2 x 105 cells/ml for three days
or at 3 x 105 cells/ml for two days.
NOTE: This maintenance schedule is appropriate for
EX-CELL CD CHO Serum-Free Medium FAQ
Density 3 x 105 cells/mL
Passage frequency 3 - 4 days
Supplements added
at time of use
8 mM L-glutamine
Environment (temperature,
C02, agitation)
37 C (humidified incubator),
8
EX-CELL MDCK Serum-Free Medium for MDCK Cells
serum.
1. Choose cultures in logarithmic growth with viabilities above
90%.
2. Prepare a freezing medium consisting of 90% cold
EX-CELLTM MDCK medium and 10% dimethyl sulfoxide
(DMSO).
3. Using
EX-CELL Sp2 0 Serum-Free Medium for Sp2 0 Cells Chemically Defined
cells from serum-supplemented medium
to EX-CELLTM Sp2/0 supplemented with 8 mM L-glutamine
at a minimum seeding density of 3 x 105 cells/mL in shaker
flasks.
2. Incubate the flasks at 37 C in a humidified
EX-CELL CD CHO Fusion - Chemically defined cell culture medium
by planting new
cultures at 4 x 105 cells/mL in 20–30 mL of
EX-CELL® CD CHO Fusion.
2. Subculture stocks every three to four days at a
seeding density of 4 x 105 cells/mL.
3. Continue stocks
EX-CELL CHO Medium (C5467)
viability is at least
90% and the cells are in the mid-logarithmic growt h
phase. Cells grown in serum-containing medium should
be inoculated at a viable cell density of 2 x 105 cells/ml
in a 1:1 mixture
Product Information Sheet - SHM02
0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x
1/16