LG, et al., Genome-scale ChIP-chip
analysis using 10,000 human cells. Biotechniques,
43:791-797 (2007).
8. O’Geen, H., et al., Genome-Wide Analysis of
KAP1 Binding Suggests Autoregulation of KRAB-
104 cells are recommended for
each ChIP sample assay well, e.g., for 8 ChIP
assay wells with 2 × 105 cells per assay, 1.6 × 106
cells should be prepared.
For monolayer or adherent cells:
Briefly
start enzymes need to be activated at
94 °C. Please see polymerase data sheet for
duration.
Thermal Cycle Program
1 cycle 94 °C for 2 minutes
39 cycles 94 °C for 30 seconds
Catalog # BCYT1-33K, BCYT1-33K-PX15, and BCYT1-33K-PXBK15
Control Catalog # BCYT1-6033 Lot, BVCYT-106 and BVCYT-206
Note: The Quality Control Ranges are generated using serum matrix provided in
Catalog # BCYT1-33K, BCYT1-33K-PX15, and BCYT1-33K-PXBK15
Control Catalog # BCYT1-6033 Lot, BVCYT-106 and BVCYT-206
Note: The Quality Control Ranges are generated using serum matrix provided in
in
RPMI Media (Gibco) containing 10% FCS and 1% Penicillin/
Streptomycin at 106 cells/mL. The PBMCs were aliquoted at 106
cells/well and incubated overnight at 37°C. The next day either PMA,
A23187
passage or a
2-day passage. Do not allow cells to
reach viable cell density (VCD) greater
than 8 × 106 viable cells/mL for
optimal performance.
2. Subculture cells for at least three
passages prior
determined in a proliferation assay using CTLL-2
indicator cells.9
Specific Activity: 1 to 1.5 x 106 Units/mg
A Unit is defined using rlL-2 as the reference in the
CTLL-2 cell proliferation assay.
assayed by flow cytometric analysis, using the
antibody at working concentration to stain 1 x 106 to
2 x 106 cells/0.1ml/test, fluorescence intensity is
observed similar to that obtained with saturating