MILLIPLEX® MAP Human Cancer Biomarker Magnetic Bead Panel 4
Kit Catalog# HCCB4MAG-58K
Control Catalog# HCC4-6058-4, Lot# HCC4-106 and HCC4-206
Note: The Quality Control Ranges are generated
degree) of 4-nitrophenol at pH 5.5, and the
millimolar absorbance coefficient of 4-nitro-
phenoxide in the presence of 0.5% DEn-CD.
References
1. Borooah, J. et al., Biochem. J., 78(1), 106-110
Neurotrophins and
the Neural Crest, Sieber-Blum, M., ed., CRC (Boca
Raton, FL: 1999), pp. 59-93.
4. Callard, R., and Gearing, A., The Cytokine Facts
Book, Academic Press (New York, N.Y.: 1994).
from BALB/c mice immunized with a
synthetic phosphorylated peptide corresponding to
amino acids 93-106 (pTyr99) of human MDMX (Gene ID:
4194). The isotype is determined using a double
diffusion immunoassay
with PBS, being careful not
to dislodge any of the cells.
3. Discard the PBS.
4. Add cell lysis buffer (106 – 107 cells/mL).
For cells in suspension
1.
approximately 1-3 x 106 cells/mL, with culture viabilities in
excess of 93%, when subcultured every 3 - 4 days (Figure 2).
The growth curve (Figure 3) indicates that maximal cell
densities nearing 5 x 106 cell/mL
et al., Proc. Nat. Acad. Sci., USA, 76,
4061 (1979).
4. Biddison, W., et al., Immunol., 131, 152 (1983).
5. Maddon, P., et al., Cell, 42, 93 (1985).
6. Dalgleish, A., et al., Nature, 312, 763 (1986)
c-myc expression in
rat colon carcinogenesis induced by
azoxymethane. J. Gastroenterol., 37, 186-93
(2002).
SS,VNC,MAM 04/19-1
2019 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
1979).
Biddison, W. E., et al., J. Immunol., 131, 152 (1983).
Maddon, P. J., et al., Cell, 42, 93 (1985).
Dalgleish, A., et al., Nature, 312, 763 (1984).
McDougal, J. S., et al., Science, 231, 382
frequently achieving densities of 4-5
x 106 cells/mL (See Figures 1A, 1B and 1C). The Sf-900 II SFM
and High Five cultures typically had cell densities ranging from
2-3.5 x 106 cells/mL. The HyQ® SFX-Insect