uncut vector DNA, such as 50 pg. Transformation efficiency of cells per microgram of DNA
>1 × 108 .
Transformation of linearized unligated vector DNA. Completeness of restriction enzyme cleavage <1%
β-mercaptoethanol to 14ml of denaturating solution per
108 cells. This solution can be stored one month at room temperature.
2. Add 10ml of solution D per 108 cells. Mix thoroughly and allow to sit 1 min at
solution.
References
1. Wilcox, P. E., Chymotrypsinogens-Chymotrypsins.
Meth. Enzymol., 19, 64-108 (1970).
2. Beeley, J. A., et al., Polyacrylamide gel isoelectric
focusing of proteins: determination
clone IM-75, purified from hybridoma cell culture
Catalog Number I1784
Product Description
Monoclonal Anti-Importin (mouse IgG2b isotype) is
derived from the hybridoma IM-75 produced by
lot-specific Certificate of Analysis)
• Particles per mL: ~5 × 108
• Particles per mg: ~5 × 108
Storage/Stability
Store this product at 2-8 °C for long-term storage.
Biophys. J., 75(3), 1163-1171
(1998).
8. Beauchamp, L. M., et al., Modifications on the
heterocyclic base of acyclovir: syntheses and
antiviral properties. J. Med. Chem., 28(8),
982-987
Potential of
Chlorophyll and Bacteriochlorophyll in Aqueous
Environment. Chem. Phys. Lett., 104(1),105-108
(1984).
4. Dijkmans, C., et al., Photosensitization of the
NAD+-Ascorbate Reaction by Lecithin Vesicles
Potential of
Chlorophyll and Bacteriochlorophyll in Aqueous
Environment. Chem. Phys. Lett., 104(1),105-108
(1984).
4. Dijkmans, C., et al., Photosensitization of the
NAD+-Ascorbate Reaction by Lecithin
structural
and functional properties of cardiocytes. The Journal of Clinical Investigation. (2001)
108:407-414.
RELATED
REFERENCES:
1. Cho, EE, et al., Arch Oral Biol (2000) 45:425-430.
2. Ohmori
25 mL
2.2. For cell culture extracts of at least 2 × 108
cells: 20 mL
OptiPrep™ Density Gradient Medium Solutions
For separation in 8 mL centrifuge tubes, prepare:
• 27.5%
Res., 132, 69-75 (1999).
4. Klee, C. et al., Adv. Enzymol., 61, 149-200 (1987).
5. Cohen, P. Ann. Rev. Biochem., 58, 453-508 (1989).
6. Wang, Y. et al., Cancer Science., 99, 1100-108
(2008).
7.
Endocrinology, 108, 395
(1981).
7. Gressens, P., et al., Nature, 362, 155-158 (1993).
8. Alle, J., et al., J. Neurooncology., 3, 197 (1985).
9. De la Fuente, M., et al., Adv. Neuroimmunol, 6, 75-
91
Endocrinology, 108, 395
(1981).
7. Gressens, P., et al., Nature, 362, 155-158 (1993).
8. Alle, J., et al., J. Neurooncology., 3, 197 (1985).
9. De la Fuente, M., et al., Adv. Neuroimmunol, 6, 75-
91
An antisense oligodeoxy-
ribonecleotide to m-calpain mRNA inhibits myoblast
fusion. J. Cell Sci., 108, 2077-2082 (1995).
12. Murray, S.S., et al., The calpain-calpastatin system
and cellular proliferation
An antisense oligodeoxy-
ribonecleotide to m-calpain mRNA inhibits myoblast
fusion. J. Cell Sci., 108, 2077-2082 (1995).
12. Murray, S.S., et al., The calpain-calpastatin system
and cellular proliferation
An antisense oligodeoxy-
ribonecleotide to m-calpain mRNA inhibits myoblast
fusion. J. Cell Sci., 108, 2077-2082 (1995).
12. Murray, S.S., et al., The calpain-calpastatin system
and cellular proliferation
An antisense oligodeoxy-
ribonecleotide to m-calpain mRNA inhibits myoblast
fusion. J. Cell Sci., 108, 2077-2082 (1995).
12. Murray, S.S., et al., The calpain-calpastatin system
and cellular proliferation