Control
Figure 2. Growth of Sf9 Cells in EX-CELL 420 and
Serum-Free Control
• Sf9 cells grown in EX-CELL 420 routinely achieve cell
densities of > 107 cells/mL with viabilities of > 95%.
• Sf9 cultures
Tomita, M., et al., Secondary structure of
sphingomyelinase from Bacillus cereus. J.
Biochem., 108, 811-815 (1990).
ESS,RBG,DMG,MAM 07/07-1
Sigma brand products are sold through Sigma-Aldrich, Inc
Tomita, M., et al., Secondary structure of
sphingomyelinase from Bacillus cereus. J.
Biochem., 108, 811-815 (1990).
ESS,RBG,DMG,MAM 07/07-1
Sigma brand products are sold through Sigma-Aldrich, Inc
incubator.
2. Wash the cells 3 X for 5 min. with PBS. Do not
shake cells.
3. Add fixative (ice-cold, 95% ethanol, 5% acetic acid)
for 1 min. at room temperature.
4. Wash the cells with PBS, 2 X
F(ab‘)2 elution pool
xMuLV (retrovirus)
Run 1: 3.3 x 108 Run 1: 1.5 x 103
5.4 ± 0.0
Run 2: 3.3 x 108 Run 2: 1.5 x 103
MVM (parvovirus)
Run 1: 1.4 x 108 Run 1: 5.5 x 105
of alanyl-glutamine on
leucine and protein metabolism in irradiated rats.
Amino Acids, 22(1), 95-108 (2002).
5. Muhling, J., et al., Effects of arginine,
L-alanyl-L-glutamine or taurine on neutrophil
and ≥ 95% viable.
2. Incubate cells at 27°C for 30 minutes to 1 hour to
allow the cells to attach to the dishes.
3. To prepare each transfection mixture, first mix 5 µl
of ESCORT IV*
Enterobacteriaceae were tested within a validation study. No signals
were observed using 2.3 x 108 Enterobacter cloacae cells or 7 x 108 Citrobacter freundii cells per assay,
whereas clear specific signals were detectable
., 207(2), 148-174 (1994).
6. Scribner, A. W., et al., The effect of angiotensin-
converting enzyme inhibition on endothelial
function and oxidant stress. Eur. J. Pharmacol.,
482(1-3), 95-99 (2003
up to 1 2 months.
Avoid repeated freeze/thaw cycles.
REFERENCES:
Li X et al. (2001). Proc Natl Acad Sci. 95(24): 14389-14394.
Salven P et al. (1998). Am J Pathol 153(1): 103-108.
: from the
natural environment to infectious diseases."
Nature reviews microbiology 2.2 (2004): 95-108.
4. Costerton, J. William, Philip S. Stewart, and E.
Peter Greenberg. "Bacterial biofilms: a
minimum detectable concentration of human LIF in this
assay is 0.5 ng/mL. The specific activity is >1 x 108 units/mg, where 50 units is defined as
the amount of human LIF required to induce differentiation
that is immunized with a synthetic
peptide corresponding to amino acid residues
GKPIPNPLLGLDST (95-108) of the P/V proteins of the
Paramyxovirus SV5. Increased expression of the
cellular oncogene V5