second microcentrifuge tube
(Blank 1 – no MKK-6).
3. Pipette 10 µL of the MKK6 and 4 µL of EDB into a
third tube (Blank 2 – no p38 MAPK).
4. Add 12 µL of the γ-32P-ATP–RB solution
Assays, Vol.
272 (J.M. Gibbins and M.P. Mahaut-Smith, eds.).
Humana Press, pp. 109-120 (2004).
GCY,MES,NSB,CMH,RXR,MAM 12/16-1
2016 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
Assays, Vol.
272 (J.M. Gibbins and M.P. Mahaut-Smith, eds.).
Humana Press, pp. 109-120 (2004).
GCY,MES,NSB,MAM 12/16-1
2016 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH
based transfection. For AAV2 3 L BR, n=6;
for AAV2 SF, n=9; for AAV5 3 L BR, n=3; for
AAV5 SF, n=6.
1.00E+08
1.00E+09
1.00E+10
1.00E+11
1.00E+12
AAV2 AAV5
Genome Titer (gc/mL)
, Nature, 304, 35 (1983).
11. Hart, C.E., et al., Science, 240, 1529 (1988).
12. Raines, E. W., et al., Methods Enzymol., 109, 749
(1985).
PHC 06/11-1
Sigma brand products are sold through Sigma-Aldrich
(2009).
3. Shav-Tal, Y., and Zipori, D., FEBS Lett., 531, 109-
114 (2002).
4. Passon, D.M., et al., Proc. Natl. Acad. Sci. USA,
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114 (2002).
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109, 4846-4850 (2012).
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Analysis of True Glucose in Plasma Without
Deproteinization. J. Clin. Pathol., 27(12),
1015-1017 (1974).
TMG/RXR 6/03
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
(1985).
14. Barnes, D., Meth. Enzymol., 163, 707 (1988).
15. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
16. Barrett, T. and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
17
HANDLING: Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
REFERENCES: 1. Int J. Oncol (1995) 6:105-109.
Important Note: During shipment, small volumes of product will
Biochemistry, 109, 475-480 (1980).
2. Fosset, M., Chappelet-Tordo, D. and Lazdunski, M., Biochemistry, 13, 1783-1788 (1974).
3. Latner, A.L., Parsons, M., and Skillen, A.W., Enzymologia, 40, 1-6 (1970).
optimal working dilutions
by titration test.
References
1. Oyler, G.A. et al., J. Cell. Biol., 109, 3039 (1989).
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3. Söllner