concentration in
the sample.
Components
The kit is sufficient for 100 assays in 96 well plates.
Dye Reagent 6 mL
Catalog Number MAK314A
10% TCA 5 mL
Catalog Number MAK314B
M1 Microplate (991AFLM01U): 96 wells
(12 8) in a microwell holder coated with a mouse
anti-aflatoxin monoclonal antibody.
2. Aflatoxin M1 Standard (993S1AFLM01U): 6 vials,
1.5 mL/vial of Aflatoxin
Microplate (951FUM01C): 96 wells
(12 8 well strips) in a microwell holder coated with
a mouse anti-fumonisin monoclonal antibody.
2. Fumonisin Standards (953S6FU01M, Black Cap):
6 vials, 1.5 mL/vial
5
11/19/96 - ARO
SODIUM NITROPRUSSIDE DIHYDRATE
Sigma Prod. No. S0501
USAGE/APPLICATIONS:
By the release of nitric oxide (NO), SNP increases the amount of 6-keto-prostaglandin F1α (6-keto-PGF1α
duplicate.
NADH Standards for Colorimetric Detection
Add 0, 2, 4, 6, 8, and 10 µL of the 1.25 mM
(1.25 nmole/µL) NADH Standard Solution into a 96 well
plate, generating 0 (blank), 2.5, 5, 7.5, 10, and
12.5
3
16. Barnes, D., Meth. Enzymol., 163, 707 (1988).
17. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
18. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
19.
105 cells/ml. After three days of growth
at 37 °C and 5% CO2, the spinners were infected with
3 x 109 virus particles (moiª3) of the rAd5 containing the
b-galactosidase (b-gal) reporter gene (Crucell
(1985).
16. Barnes, D., Meth. Enzymol., 163, 707 (1988).
17. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
18. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
19.
(1985).
15. Barnes, D., Meth. Enzymol., 163, 707 (1988).
16. Pierce, G., et al., J. Cell Biol., 109, 429 (1989).
17. Barrett, T., and Benditt, E., Proc. Natl. Acad. Sci.
USA, 85, 2810 (1988).
18.
A., et al., Cell, 96, 677-687 (1999).
4. Beattie, E.C., et al., J. Neurosci., 20, 7325-7333
(2000).
5. Mallet, W.G., and Brodsky, F.M., J. Cell Sci., 109,
3059-3068 (1996).
6. Pearse, B.M., and
Positive Control (Part No. AMP4200-A)
250 µL, 109 copies/5 µL. Store at or below -20°C.
6. HRV Type B Positive Control (Part No. AMP4200-B)
250 µL, 109 copies/5 µL. Store at
1, immediately after seeding at
3 x 105 cells/mL (Figure 2). Viral titers of approximately
1.5 x 109 TCID50/mL were obtained in EX-CELL™ HeLa. These
results were within one-half log value of those seen
Interaction, New York: Springer-Verlag,
p. 206 (1979).
8. Orr-Urtreger, A., et al., Development, 109, 911
(1990).
9. Manova, K., et al., Development, 110, 1057 (1990).
10. Arakawa, T., et al., J. Biol
the user. It is recommended that no more
than 109 bacterial cells be used in this procedure. As a
general rule, an E. coli culture containing
1 × 109 cells/mL has an OD600 of 1.0 as per standard
spectrophotometers
of accurately measuring 1-1000 µL
4. Graduated serological pipettes
5. 96-well microtiter Plate Reader with 450 nm filter
6. Graphing software for plotting data or graph paper for manual plotting of
reader capable of reading absorbance at
450 nm
5. Absorbent paper or paper towel
6. Graph paper
Materials Provided 96 Tests
Microwell coated with murine
monoclonal anti-myoglobin. 12 x 8 x 1
Reference