11890

应用筛选条件

关键词:'11890'

显示 1-30 共 31 条结果关于 "11890" 范围论文

Metal contaminants promote degradation of lipid/DNA complexes during lyophilization.

Marion d C Molina et al.

Biochimica et biophysica acta, 1768(3), 669-677 (2007-01-17)

Oxidation reactions represent an important degradation pathway of nucleic acid-based pharmaceuticals. To evaluate the role of metal contamination and chelating agents in the formation of reactive oxygen species (ROS) during lyophilization, ROS generation and the stability of lipid/DNA complexes were

The rutin/beta-cyclodextrin interactions in fully aqueous solution: spectroscopic studies and biological assays.

M L Calabrò et al.

Journal of pharmaceutical and biomedical analysis, 36(5), 1019-1027 (2004-12-29)

In the present work the feasibility of beta-cyclodextrin complexation was explored, as a tool for improving the aqueous solubility and antioxidant efficacy of rutin. By means of 1H NMR, UV-vis and circular dichroism spectroscopy the single aromatic ring of rutin

Determination of iron with bathophenanthroline without deproteinisation.

J Bouda

Clinica chimica acta; international journal of clinical chemistry, 21(1), 159-160 (1968-07-01)

Ferric and cupric reductase activities by iron-limited cells of the green alga Chlorella kessleri: quantification via oxygen electrode.

Harold G Weger et al.

Physiologia plantarum, 131(2), 322-331 (2008-02-07)

The colorimetric Fe2+ indicators bathophenanthroline disulfonic acid (BPDS) and 3-(2-pyridyl)-5,6-bis(4-phenylsulfonic acid)-1,2,4-triazine (FZ) are routinely used to assay for plasma membrane ferric reductase activity in iron-limited algal cells and also in roots from iron-limited plants. Ferric reductase assays using these colorimetric

K. Miyaishi et al.

Analytica Chimica Acta, 124, 381-381 (1981)

Ternary complex formation facilitates a redox mechanism for iron release from a siderophore.

Kassy A Mies et al.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 19(2), 115-126 (2006-05-24)

While the naturally occurring reducing agents glutathione (GSH) and ascorbate (H2A) alone are ineffective at reducing iron(III) sequestered by the siderophore ferrioxamine B, the addition of an iron(II) chelator, sulfonated bathophenanthroline (BPDS), facilitates reduction by either reducing agent. A mechanism

Chemiluminescence reactions with cationic, neutral, and anionic ruthenium(II) complexes containing 2,2'-bipyridine and bathophenanthroline disulfonate ligands.

Paul S Francis et al.

Talanta, 82(2), 859-862 (2010-07-07)

Ruthenium complexes containing 4,7-diphenyl-1,10-phenanthroline disulfonate (bathophenanthroline disulfonate; BPS) ligands, Ru(BPS)(3)(4-), Ru(BPS)(2)(bipy)(2-) and Ru(BPS)(bipy)(2), were compared to tris(2,2'-bipyridine)ruthenium(II) (Ru(bipy)(3)(2+)), including examination of the wavelengths of maximum absorption and corrected emission intensity, photoluminescence quantum yield, stability of their oxidised ruthenium(III) form, and

Two iron-regulated cation transporters from tomato complement metal uptake-deficient yeast mutants.

U Eckhardt et al.

Plant molecular biology, 45(4), 437-448 (2001-05-16)

Although iron deficiency poses severe nutritional problems to crop plants, to date iron transporters have only been characterized from the model plant Arabidopsis thaliana. To extend our molecular knowledge of Fe transport in crop plants, we have isolated two cDNAs

Luminescent iridium(III) complexes as novel protein staining agents.

Junli Jia et al.

Electrophoresis, 33(9-10), 1397-1401 (2012-06-01)

This article reports a new class of luminescent metal complexes, biscyclometalated iridium(III) complexes with an ancillary bathophenanthroline disulfonate ligand, for staining protein bands that are separated by electrophoresis. The performances of these novel staining agents have been studied in comparison

Double electrochemical covalent coupling method based on click chemistry and diazonium chemistry for the fabrication of sensitive amperometric immunosensor.

Honglan Q

Analytica Chimica Acta, 729, 28-34 (2013)

Reduction of transition metals by human (THP-1) monocytes is enhanced by activators of protein kinase C.

J L Wood et al.

Free radical research, 31(5), 367-379 (1999-11-05)

Macrophages oxidize low density lipoprotein (LDL) by enzymatic and non-enzymatic mechanisms; however, it is evident that macrophage reduction of transition metals can accelerate LDL oxidation in vitro, and possibly in vivo. Distinct cellular pathways contribute to this process, including trans-plasma

Evidence that intrinsic iron but not intrinsic copper determines S-nitrosocysteine decomposition in buffer solution.

Anatoly F Vanin et al.

Nitric oxide : biology and chemistry, 7(3), 194-209 (2002-10-17)

The present experiments were designed to analyze the influence of copper and iron ions on the process of decomposition of S-nitrosocysteine (cysNO), the most labile species among S-nitrosothiols (RSNO). CysNO fate in buffer solution was evaluated by optical and electron

Comparison of colorimetry and electrothermal atomic absorption spectroscopy for the quantification of non-transferrin bound iron in human sera.

Piyada Jittangprasert et al.

The Southeast Asian journal of tropical medicine and public health, 35(4), 1039-1044 (2005-05-27)

This paper describes a comparison of two analytical techniques, one employing bathophenanthrolinedisulfonate (BPT), a most commonly-used reagent for Fe (II) determination, as chromogen and an electrothermal atomic absorption spectroscopy (ETAAS) for the quantification of non-transferrin bound iron (NTBI) in sera

Association of redox-active iron bound to high molecular weight structures in nuclei with inhibition of cell growth by H2O2.

H Li et al.

Free radical biology & medicine, 26(1-2), 49-60 (1999-01-16)

Perturbations to Fe species contributing to generation of DNA single-strand breaks (SSBs) and inhibition of growth by H2O2 were studied in HL-60 cells made Fe-deficient by 24 h pretreatment with 144 microM bathophenanthroline disulfonic acid and 400 microM ascorbic acid

Nontransferrin-bound iron uptake by hepatocytes is increased in the Hfe knockout mouse model of hereditary hemochromatosis.

Anita C G Chua et al.

Blood, 104(5), 1519-1525 (2004-05-25)

Hereditary hemochromatosis (HH) is an iron-overload disorder caused by a C282Y mutation in the HFE gene. In HH, plasma nontransferrin-bound iron (NTBI) levels are increased and NTBI is bound mainly by citrate. The aim of this study was to examine

Phenylalanine hydroxylase. Correlation of the iron content with activity and the preparation and reconstitution of the apoenzyme.

D W Gottschall et al.

The Journal of biological chemistry, 257(2), 845-849 (1982-01-25)

Phenylalanine hydroxylase requires 1.0 mol of iron/Mr = 50,000 subunit for maximal activity. A direct correlation between iron content and specific activity has been demonstrated through a comparison of enzyme activity and iron bound per subunit for various enzyme preparations

Gamma-glutamyl transpeptidase-dependent iron reduction and LDL oxidation--a potential mechanism in atherosclerosis.

A Paolicchi et al.

Journal of investigative medicine : the official publication of the American Federation for Clinical Research, 47(3), 151-160 (1999-04-13)

gamma-Glutamyl transpeptidase (gamma-GT) is found in serum and in the plasma membranes of virtually all cell types. Its physiologic role is to initiate the hydrolysis of extracellular glutathione (GSH), a tripeptide in which cysteine lies between alpha-glycine and gamma-glutamate residues.

Quantitative proteome analysis in benign thyroid nodular disease using the fluorescent ruthenium II tris(bathophenanthroline disulfonate) stain.

K Berger et al.

Molecular and cellular endocrinology, 227(1-2), 21-30 (2004-10-27)

Thyroid tumorigenesis involves qualitative and quantitative changes in protein expression, which can be comprehensively studied by proteome analysis. However, one of the technical bottlenecks of proteomics remains a reliable, sensitive and inexpensive method for quantification of differentially expressed proteins. This

Green chemiluminescence from a bis-cyclometalated iridium(III) complex with an ancillary bathophenanthroline disulfonate ligand.

Elizabeth M Zammit et al.

The Analyst, 136(15), 3069-3072 (2011-06-29)

The reaction of a fluorinated iridium complex with cerium(IV) and organic reducing agents generates an intense emission with a significant hypsochromic shift compared to contemporary chemically-initiated luminescence from metal complexes.

Increased divalent metal transporter 1 expression might be associated with the neurotoxicity of L-DOPA.

Yan-Zhong Chang et al.

Molecular pharmacology, 69(3), 968-974 (2005-12-01)

Based on the available data, we speculated that changes in brain iron metabolism induced by L-DOPA might be associated with the neurotoxicity of L-DOPA. To investigate this possibility, the effects of L-DOPA on the expression of iron influx proteins [transferrin

Mechanisms of ferric citrate uptake by human hepatoma cells.

D Trinder et al.

The American journal of physiology, 275(2 Pt 1), G279-G286 (1998-08-04)

The mechanisms of uptake of non-transferrin-bound iron by human hepatoma cells (HuH7) were investigated using 59Fe-citrate and [14C]citrate. The amount of iron associated with the cells increased linearly with time, whereas citrate uptake reached a plateau after 45 min, resulting

Zinc inhibits oxidative stress-induced iron signaling and apoptosis in Caco-2 cells.

Sreenivasulu Kilari et al.

Free radical biology & medicine, 48(7), 961-968 (2010-01-26)

Studies in humans and animals have suggested negative interactions of iron and zinc during their intestinal absorption. Further, zinc seems to prevent iron-induced oxidative damage in rats, which was hypothesized to be through the modulation of the intracellular iron signaling

Dinitrosyl iron complexes with thiol-containing ligands and apoptosis: studies with HeLa cell cultures.

Nadezhda Ya Giliano et al.

Nitric oxide : biology and chemistry, 24(3), 151-159 (2011-03-01)

No pro-apoptotic effect of dinitrosyl iron complexes (DNIC) with glutathione, cysteine or thiosulfate was established after incubation of HeLa cells in Eagle's medium. However, DNIC with thiosulfate manifested pro-apoptotic activity during incubation of HeLa cells in Versene's solution supplemented with

NMR study on iridium(III) complexes for identifying disulfonate substituted bathophenanthroline regio-isomers.

Chenchen Liu et al.

Magnetic resonance in chemistry : MRC, 49(12), 816-823 (2011-11-15)

A series of novel biscyclometalated iridium (III) complexes with an ancillary disulfonated bathophenanthroline (DSBP(2-)) ligand, Ir(L)(2)DSBPNa, L = 2-phenylpyridine (ppy), 2,4-difluorophenylpyridine (fppy), and 1-phenylisoquinoline (piq) were found to have two isomeric forms. The chemical structures of the isomers were determined

Use of a fluorescent internal protein standard to achieve quantitative two-dimensional gel electrophoresis.

Asa M Wheelock et al.

Proteomics, 6(5), 1385-1398 (2006-01-24)

2-DE is a powerful separation method for complex protein mixtures. However, large intergel variations in spot intensity limit its use for quantitative proteomics studies. To address this issue, we developed a fluorescent internal protein standard for use in 2-DE analysis.

Role of ascorbic acid in transferrin-independent reduction and uptake of iron by U-937 cells.

J M May et al.

Biochemical pharmacology, 57(11), 1275-1282 (1999-05-07)

The role of ascorbic acid in transferrin-independent ferric iron reduction and uptake was evaluated in cultured U-937 monocytic cells. Uptake of 55Fe by U-937 cells was doubled by 100 microM extracellular ascorbate, and by pre-incubation of cells with 100 microM

Selective functionalization of independently addressed microelectrodes by electrochemical activation and deactivation of a coupling catalyst.

Neal K Devaraj et al.

Journal of the American Chemical Society, 128(6), 1794-1795 (2006-02-09)

We demonstrate selective functionalization of independently addressed microelectrodes by electrochemical activation and deactivation of a coupling catalyst. 1,2,3-Triazole formation between terminal acetylenes and organic azides is efficiently catalyzed by copper(I) complexes (a Sharpless "click" reaction), while the oxidized copper(II) complexes

D. Blair et al.

Talanta, 7, 163-163 (1961)

Electrochemistry and electrochemiluminescence of [Ru(II)-tris(bathophenanthroline-disulfonate)]4- in aprotic conditions and aqueous buffers.

Simone Zanarini et al.

The journal of physical chemistry. B, 112(33), 10188-10193 (2008-07-26)

In this work, the electrochemical and ECL properties of tris[1,10-phenanthrolinediyl-4,7-di(benzenesulfonate)]Ru(II) ([Ru(BPS)3]4-) have been addressed in both strictly aprotic conditions and aqueous buffers. A combined theoretical and experimental approach is presented to focus thermodynamics and kinetic effects of electro-generated species possessing

Vasorelaxing activity of stable powder preparations of dinitrosyl iron complexes with cysteine or glutathione ligands.

Anatoly F Vanin et al.

Nitric oxide : biology and chemistry, 16(3), 322-330 (2007-01-30)

Vasorelaxant activity of new stable powder preparations of dinitrosyl iron complexes (DNIC) with thiol-containing ligands was investigated on rat abdominal aorta rings. The preparations preserve their physicochemical characteristics (EPR and optical absorption) if stored for a long time in dry

1/2