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164739
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关键词:'164739'
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Adam G Larson et al.
Biophysical journal, 98(11), 2619-2627 (2010-06-02)
Kinesin superfamily motor proteins contain a structurally conserved loop near the ATP binding site, termed L5. The function of L5 is unknown, although several drug inhibitors of the mitotic kinesin Eg5 bind to L5. We used electron paramagnetic resonance spectroscopy
Ahmed Abdelbaki et al.
Journal of cell science, 133(12) (2020-05-13)
Activity of AURKA is controlled through multiple mechanisms including phosphorylation, ubiquitin-mediated degradation and allosteric interaction with TPX2. Activity peaks at mitosis, before AURKA is degraded during and after mitotic exit in a process strictly dependent on the APC/C coactivator FZR1.
Makiko Shimizu et al.
Bioorganic & medicinal chemistry letters, 20(5), 1578-1580 (2010-02-09)
Biochemical analysis of the cellular target of S-trityl-l-cysteine (STLC) derivatives was performed by using the newly synthesized STLC derivative-immobilized affinity beads (3d). The affinity beads efficiently captured KSP in HCT116 cytoplasmic cell lysate. The results obtained from pull-down and competition
Nurhan Ozlü et al.
Molecular & cellular proteomics : MCP, 9(2), 336-350 (2009-09-30)
The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that
Julien Aureille et al.
EMBO reports, 20(9), e48084-e48084 (2019-08-02)
The shape of the cell nucleus can vary considerably during developmental and pathological processes; however, the impact of nuclear morphology on cell behavior is not known. Here, we observed that the nuclear envelope flattens as cells transit from G1 to
Katerina Jerabkova et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(9), 12751-12767 (2020-08-02)
Equal segregation of chromosomes during mitosis ensures euploidy of daughter cells. Defects in this process may result in an imbalance in the chromosomal composition and cellular transformation. Proteolytic and non-proteolytic ubiquitylation pathways ensure directionality and fidelity of mitotic progression but
Ingrid E Adriaans et al.
The Journal of cell biology, 218(4), 1250-1264 (2019-02-08)
Cytokinesis begins upon anaphase onset. An early step involves local activation of the small GTPase RhoA, which triggers assembly of an actomyosin-based contractile ring at the equatorial cortex. Here, we delineated the contributions of PLK1 and Aurora B to RhoA
Bernd Thiede et al.
Molecular & cellular proteomics : MCP, 12(2), 529-538 (2012-10-04)
The proteomics field has shifted over recent years from two-dimensional gel electrophoresis (2-DE)-based approaches to SDS-PAGE or gel-free workflows because of the tremendous developments in isotopic labeling techniques, nano-liquid chromatography, and high-resolution mass spectrometry. However, 2-DE still offers the highest
Total synthesis of didmolamides A and B
You Shu-Li and Kelly JW
Tetrahedron Letters, 46(15), 2567-2570 (2005)
Chieh-Ting Fang et al.
Cell death & disease, 11(8), 715-715 (2020-09-03)
The heat shock protein 70 (HSP70) is a conserved molecular chaperone and proteostasis regulator that protects cells from pharmacological stress and promotes drug resistance in cancer cells. In this study, we found that HSP70 may promote resistance to anticancer drugs
Junbin Qian et al.
Molecular cell, 68(4), 715-730 (2017-11-14)
The spindle assembly checkpoint (SAC) generates a diffusible protein complex that prevents anaphase until all chromosomes are properly attached to spindle microtubules. A key step in SAC initiation is the recruitment of MAD1 to kinetochores, which is generally thought to
Katarzyna Majstrowicz et al.
Journal of cell science, 134(10) (2021-05-21)
Myosin XIX (Myo19) is an actin-based motor that competes with adaptors of microtubule-based motors for binding to the outer mitochondrial transmembrane proteins Miro1 and Miro2 (collectively Miro, also known as RhoT1 and RhoT2, respectively). Here, we investigate which mitochondrial and
James A D Good et al.
Journal of medicinal chemistry, 56(5), 1878-1893 (2013-02-12)
The mitotic kinesin Eg5 is critical for the assembly of the mitotic spindle and is a promising chemotherapy target. Previously, we identified S-trityl-L-cysteine as a selective inhibitor of Eg5 and developed triphenylbutanamine analogues with improved potency, favorable drug-like properties, but
Frank Kozielski et al.
Proteomics, 8(2), 289-300 (2008-01-11)
Mitotic kinesins represent potential drug targets for anticancer chemotherapy. Inhibitors of different chemical classes have been identified that target human Eg5, a kinesin responsible for the establishment of the bipolar spindle. One potent Eg5 inhibitor is S-trityl-L-cysteine (STLC), which arrests
Sei Shu et al.
Journal of cell science, 132(24) (2019-11-24)
Chromosomal instability, one of the most prominent features of tumour cells, causes aneuploidy. Tetraploidy is thought to be an intermediate on the path to aneuploidy, but the mechanistic relationship between the two states is poorly understood. Here, we show that
Marion A A Libouban et al.
Oncotarget, 8(24), 38309-38325 (2017-04-19)
Inhibition of the spindle assembly checkpoint kinase TTK causes chromosome mis-segregation and tumor cell death. However, high levels of TTK correlate with chromosomal instability (CIN), which can lead to aneuploidy. We show that treatment of tumor cells with the selective
Yusuke Toyoda et al.
Nature communications, 8(1), 1266-1266 (2017-11-04)
To divide, most animal cells drastically change shape and round up against extracellular confinement. Mitotic cells facilitate this process by generating intracellular pressure, which the contractile actomyosin cortex directs into shape. Here, we introduce a genome-scale microcantilever- and RNAi-based approach
Zhihao Tan et al.
Molecular biology of the cell, 30(1), 42-55 (2018-11-01)
Understanding how cells acquire genetic mutations is a fundamental biological question with implications for many different areas of biomedical research, ranging from tumor evolution to drug resistance. While karyotypic heterogeneity is a hallmark of cancer cells, few mutations causing chromosome
Yuki Ikeda et al.
International journal of molecular sciences, 22(11) (2021-06-03)
Insulin-like growth factor 1 receptor (IGF1R), a receptor-type tyrosine kinase, transduces signals related to cell proliferation, survival, and differentiation. We recently reported that OSI-906, an IGF1R inhibitor, in combination with the Aurora B inhibitor ZM447439 suppresses cell proliferation. However, the
Christina L Hueschen et al.
Current biology : CB, 29(4), 700-708 (2019-02-13)
Each time a cell divides, the microtubule cytoskeleton self-organizes into the metaphase spindle: an ellipsoidal steady-state structure that holds its stereotyped geometry despite microtubule turnover and internal stresses [1-6]. Regulation of microtubule dynamics, motor proteins, microtubule crosslinking, and chromatid cohesion can
Makiko Shimizu et al.
Cancer letters, 298(1), 99-106 (2010-07-14)
Effect of CF(3)-STLC, a potent kinesin spindle protein (KSP) inhibitor, on K562 human CML cell line was investigated. Treatment with CF(3)-STLC induced mitotic arrest of the cell cycle with the appearance of characteristic monoastral spindles, subsequent apoptotic cell death and
Erik Müllers et al.
Cell cycle (Georgetown, Tex.), 13(17), 2733-2743 (2014-12-09)
Upon DNA damage, cell cycle progression is temporally blocked to avoid propagation of mutations. While transformed cells largely maintain the competence to recover from a cell cycle arrest, untransformed cells past the G1/S transition lose mitotic inducers, and thus the
Nicolas Taulet et al.
Nature communications, 8(1), 1928-1928 (2017-12-06)
Cytokinesis mediates the physical separation of dividing cells and, in 3D epithelia, provides a spatial landmark for lumen formation. Here, we unravel an unexpected role in cytokinesis for proteins of the intraflagellar transport (IFT) machinery, initially characterized for their ciliary
Hua Shao et al.
The Journal of cell biology, 201(2), 191-200 (2013-04-10)
The spindle assembly checkpoint (SAC) functions as a surveillance mechanism to detect chromosome misalignment and to delay anaphase until the errors are corrected. The SAC is thought to control mitosis and meiosis, including meiosis in mammalian eggs. However, it remains
Kamran Hosseini et al.
Biophysical journal, 119(6), 1091-1107 (2020-08-28)
Mechanosensation of cells is an important prerequisite for cellular function, e.g., in the context of cell migration, tissue organization, and morphogenesis. An important mechanochemical transducer is the actin cytoskeleton. In fact, previous studies have shown that actin cross-linkers such as
Riya Keshri et al.
Journal of cell science, 133(14) (2020-06-28)
Proper orientation of the mitotic spindle is critical for accurate development and morphogenesis. In human cells, spindle orientation is regulated by the evolutionarily conserved protein NuMA, which interacts with dynein and enriches it at the cell cortex. Pulling forces generated
Alberto Camaleño de la Calle et al.
Macromolecular bioscience, 19(6), e1900033-e1900033 (2019-04-13)
Binding of mannose presenting macromolecules to the protein receptor concanavalin A (ConA) is investigated by means of single-molecule atomic force spectroscopy (SMFS) in combination with dynamic light scattering and molecular modeling. Oligomeric (Mw ≈ 1.5-2.5 kDa) and polymeric (Mw ≈
Murad N Abualhasan et al.
European journal of medicinal chemistry, 54, 483-498 (2012-07-04)
S-Trityl L-cysteine (STLC) is an inhibitor of the mitotic kinesin Eg5 with potential as an antimitotic chemotherapeutic agent. We previously reported the crystal structure of the ligand-protein complex, and now for the first time, have quantified the interactions using a
Christian J Koehler et al.
Analytical chemistry, 83(12), 4775-4781 (2011-05-03)
Recently, we introduced a novel approach for protein quantification based on isobaric peptide termini labeling (IPTL). In IPTL, both peptide termini are dervatized in two separate chemical reactions with complementary isotopically labeled reagents to generate isobaric peptide pairs. Here, we
Marcel Mokbel et al.
Biophysical journal, 118(8), 1968-1976 (2020-03-26)
Cell shape changes are vital for many physiological processes such as cell proliferation, cell migration, and morphogenesis. They emerge from an orchestrated interplay of active cellular force generation and passive cellular force response, both crucially influenced by the actin cytoskeleton.
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