199664

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Sudan black B reduces autofluorescence in murine renal tissue.

Yan Sun et al.

Archives of pathology & laboratory medicine, 135(10), 1335-1342 (2011-10-06)

Renal tissue emits intense autofluorescence, making it difficult to differentiate specific immunofluorescence signals and thus limiting its application to clinical biopsy material. To identify and minimize autofluorescence of renal tissue and demonstrate a simple, efficient method to reduce autofluorescence using

Non-neutralizing Antibodies from a Marburg Infection Survivor Mediate Protection by Fc-Effector Functions and by Enhancing Efficacy of Other Antibodies.

Philipp A Ilinykh et al.

Cell host & microbe, 27(6), 976-991 (2020-04-23)

Marburg virus (MARV) and Ebola virus (EBOV) belong to the family Filoviridae. MARV causes severe disease in humans with high fatality. We previously isolated a large panel of monoclonal antibodies (mAbs) from B cells of a human survivor with previous

Leptin Resistance in the Ovary of Obese Mice is Associated with Profound Changes in the Transcriptome of Cumulus Cells.

Karolina Wołodko et al.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 54(3), 417-437 (2020-04-30)

Obesity is associated with infertility, decreased ovarian performance and lipotoxicity. However, little is known about the aetiology of these reproductive impairments. Here, we hypothesise that the majority of changes in ovarian physiology in diet-induced obesity (DIO) are a consequence of

A New Method to Stabilize C-Kit Expression in Reparative Cardiac Mesenchymal Cells.

Marcin Wysoczynski et al.

Frontiers in cell and developmental biology, 4, 78-78 (2016-08-19)

Cell therapy improves cardiac function. Few cells have been investigated more extensively or consistently shown to be more effective than c-kit sorted cells; however, c-kit expression is easily lost during passage. Here, our primary goal was to develop an improved

Thermoneutrality-Induced Macrophage Accumulation in Brown Adipose Tissue Does Not Impair the Tissue's Competence for Cold-Induced Thermogenic Recruitment.

Alexander W Fischer et al.

Frontiers in endocrinology, 11, 568682-568682 (2020-11-17)

Brown adipose tissue from mice living under conditions approaching human thermal and nutritional conditions (prolonged exposure to thermoneutral temperature and to an energy-rich (high-fat, high-sugar) diet) - referred to as "physiologically humanized" mice, displays morphological and molecular characteristics significantly different

Acute ischemic stroke induces magnetic resonance susceptibility signs dominated by endothelial nitric oxide synthase activation.

Chiao-Chi V Chen et al.

Magnetic resonance in medicine, 85(4), 2201-2211 (2020-11-01)

Acute ischemic stroke induces deoxyhemoglobin accumulation around the ischemic region while activating endothelial nitric oxide synthase (eNOS) coupling and the subsequent release of nitric oxide (NO). Because deoxyhemoglobin is a natural NO spin trap, its interplay with NO could be

Cortical neurons develop a senescence-like phenotype promoted by dysfunctional autophagy.

Daniel Moreno-Blas et al.

Aging, 11(16), 6175-6198 (2019-08-31)

Senescent cells accumulate in various tissues and organs with aging altering surrounding tissue due to an active secretome, and at least in mice their elimination extends healthy lifespan and ameliorates several chronic diseases. Whether all cell types senesce, including post-mitotic

Barley grain with adhering hulls is controlled by an ERF family transcription factor gene regulating a lipid biosynthesis pathway.

Shin Taketa et al.

Proceedings of the National Academy of Sciences of the United States of America, 105(10), 4062-4067 (2008-03-05)

In contrast to other cereals, typical barley cultivars have caryopses with adhering hulls at maturity, known as covered (hulled) barley. However, a few barley cultivars are a free-threshing variant called naked (hulless) barley. The covered/naked caryopsis is controlled by a

Solving underwater crimes: development of latent prints made on submerged objects.

Ana Castelló et al.

Science & justice : journal of the Forensic Science Society, 53(3), 328-331 (2013-08-14)

Underwater crime scenes always present a challenge for forensic researchers, as the destructive effect of water considerably complicates the chances of recovering material of evidential value. The aim of this study is to tackle the problem of developing marks that

Spontaneously immortalized Schwann cells from adult Fischer rat as a valuable tool for exploring neuron-Schwann cell interactions.

Kazunori Sango et al.

Journal of neuroscience research, 89(6), 898-908 (2011-03-12)

We established spontaneously immortalized Schwann cell lines from long-term cultures of adult Fischer 344 rat dorsal root ganglia (DRG) and peripheral nerves. One of these cell lines, designated immortalized Fischer rat Schwann cells 1 (IFRS1), showed spindle-shaped morphology; immunoreactivity for

Cytochemical characterisation of the leucocytes and thrombocytes from Murray cod (Maccullochella peelii peelii, Mitchell).

Sarah Shigdar et al.

Fish & shellfish immunology, 26(5), 731-736 (2009-04-01)

Cytochemistry has proven effective in differentiating specific cell lineages and elucidating their functional properties. This study utilised a range of cytochemical techniques to further investigate the leucocyte populations from Murray cod, an iconic Australian teleost fish species. This analysis provided

Behavioral and neural network abnormalities in human APP transgenic mice resemble those of App knock-in mice and are modulated by familial Alzheimer's disease mutations but not by inhibition of BACE1.

Erik C B Johnson et al.

Molecular neurodegeneration, 15(1), 53-53 (2020-09-15)

Alzheimer's disease (AD) is the most frequent and costly neurodegenerative disorder. Although diverse lines of evidence suggest that the amyloid precursor protein (APP) is involved in its causation, the precise mechanisms remain unknown and no treatments are available to prevent

Antioxidant sestrin-2 redistribution to neuronal soma in human immunodeficiency virus-associated neurocognitive disorders.

Virawudh Soontornniyomkij et al.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 7(3), 579-590 (2012-03-28)

Sestrin-2 is involved in p53-dependent antioxidant defenses and in the maintenance of metabolic homeostasis. We hypothesize that sestrin-2 expression is altered in the brains of subjects diagnosed with human immunodeficiency virus (HIV)-associated neurocognitive disorders (HAND) due to neuronal oxidative stress.

Distinct domanial and lamellar distribution of clustered lipofuscin granules in microglia in the main olfactory bulb of young mice.

Katsuko Kosaka et al.

Neuroscience research, 65(3), 286-295 (2009-08-12)

Lipofuscin granules are generally considered as age-pigment. However, we encountered numerous large irregular clusters of lipofuscin granules in the olfactory nerve layer and glomerular layer of the main olfactory bulb (MOB) of young adult and even juvenile mice of C57BL/6J

Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function.

Amanda J Eakin et al.

Frontiers in cell and developmental biology, 8, 799-799 (2020-09-26)

Anthracycline-based chemotherapy is a common treatment for cancer patients. Because it is delivered intravenously, endothelial cells are exposed first and to the highest concentrations, prior to diffusion to target cells. Not surprisingly, vascular dysfunction is a consequence of anthracycline therapy.

Postmitotic neurons develop a p21-dependent senescence-like phenotype driven by a DNA damage response.

Diana Jurk et al.

Aging cell, 11(6), 996-1004 (2012-08-14)

In senescent cells, a DNA damage response drives not only irreversible loss of replicative capacity but also production and secretion of reactive oxygen species (ROS) and bioactive peptides including pro-inflammatory cytokines. This makes senescent cells a potential cause of tissue

Does Seipin Play a Role in Oxidative Stress Protection and Peroxisome Biogenesis? New Insights from Human Brain Autopsies.

Sofía Sánchez-Iglesias et al.

Neuroscience, 396, 119-137 (2018-11-18)

Seipin is a widely expressed protein but with highest levels found in the brain and testes. Seipin function is not yet completely understood, therefore the aim of this study was to evaluate the expression of BSCL2 transcripts in the central

Generation of neighbor-labeling cells to study intercellular interactions in vivo.

Luigi Ombrato et al.

Nature protocols, 16(2), 872-892 (2020-12-15)

Understanding cell-cell interactions is critical in most, if not all, research fields in biology. Nevertheless, studying intercellular crosstalk in vivo remains a relevant challenge, due mainly to the difficulty in spatially locating the surroundings of particular cells in the tissue.

Loss of O-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration.

Andrew C Wang et al.

Proceedings of the National Academy of Sciences of the United States of America, 113(52), 15120-15125 (2016-12-14)

O-GlcNAc glycosylation (or O-GlcNAcylation) is a dynamic, inducible posttranslational modification found on proteins associated with neurodegenerative diseases such as α-synuclein, amyloid precursor protein, and tau. Deletion of the O-GlcNAc transferase (ogt) gene responsible for the modification causes early postnatal lethality

An Alternative to Dye-Based Approaches to Remove Background Autofluorescence From Primate Brain Tissue.

Wonn S Pyon et al.

Frontiers in neuroanatomy, 13, 73-73 (2019-08-06)

Brain tissue contains autofluorescing elements that potentially impede accurate identification of neurons when visualized with fluorescent microscopy. Age-related accumulation of molecules with autofluorescent properties, such as lipofuscin, can possess spectral profiles that invade the typical emission range of fluorophores commonly

Glomerular Complement Factor H-Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment.

Nicholas R Medjeral-Thomas et al.

Kidney international reports, 4(10), 1387-1400 (2019-11-09)

Therapeutic agents that target complement are increasingly available for glomerular diseases. However, the mechanisms linking glomerular complement deposition with inflammation and damage are incompletely understood. Complement factor H-related protein 5 (FHR5) interacts with complement C3 and is considered to promote

John D. Bancroft

Theory and Practice of Histological Techniques (2008)

Spatio-temporal and Cellular Expression Patterns of PTK7 in the Healthy and Traumatically Injured Rat and Human Spinal Cord.

Pau González et al.

Cellular and molecular neurobiology, 40(7), 1087-1103 (2020-01-25)

Despite the emerging role of protein tyrosine kinase 7 (PTK7) as a Wnt co-receptor and the relevant functions of the Wnt family of proteins in spinal cord injury (SCI), the potential involvement of PTK7 in SCI is currently unknown. As

Localization of Phosphoproteins within the Barnacle Adhesive Interface.

Gary H Dickinson et al.

The Biological bulletin, 230(3), 233-242 (2016-07-02)

Barnacles permanently adhere to nearly any inert substrate using proteinaceous glue. The glue consists of at least ten major proteins, some of which have been isolated and sequenced. Questions still remain about the chemical mechanisms involved in adhesion and the

Synaptic protein changes after a chronic period of sensorimotor perturbation in adult rats: a potential role of phosphorylation/O-GlcNAcylation interplay.

Julie Fourneau et al.

Journal of neurochemistry, 147(2), 240-255 (2018-05-29)

In human, a chronic sensorimotor perturbation (SMP) through prolonged body immobilization alters motor task performance through a combination of peripheral and central factors. Studies performed on a rat model of SMP have shown biomolecular changes and a reorganization of sensorimotor

Dchs1-Fat4 regulation of osteogenic differentiation in mouse.

Ivan Crespo-Enriquez et al.

Development (Cambridge, England), 146(14) (2019-07-31)

In human, mutations of the protocadherins FAT4 and DCHS1 result in Van Maldergem syndrome, which is characterised, in part, by craniofacial abnormalities. Here, we analyse the role of Dchs1-Fat4 signalling during osteoblast differentiation in mouse. We show that Fat4 and

An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalin-fixed paraffin-embedded tissues.

M S Viegas et al.

European journal of histochemistry : EJH, 51(1), 59-66 (2007-06-06)

Interference by autofluorescence is one of the major shortcomes of immunofluorescence analysis by confocal laser scanning microscopy (CLSM). CLSM requires minimal tissue autofluorescence and reduced unspecific fluorescence background, requisites that become more critical when direct immunofluorescence studies are concerned. To

Evaluation of Myelin Radiotracers in the Lysolecithin Rat Model of Focal Demyelination: Beware of Pitfalls!

Min Zhang et al.

Contrast media & molecular imaging, 2019, 9294586-9294586 (2019-07-10)

The observation that amyloid radiotracers developed for Alzheimer's disease bind to cerebral white matter paved the road to nuclear imaging of myelin in multiple sclerosis. The lysolecithin (lysophosphatidylcholine (LPC)) rat model of demyelination proved useful in evaluating and comparing candidate

Chemerin receptor blockade improves vascular function in diabetic obese mice via redox-sensitive and Akt-dependent pathways.

Karla Bianca Neves et al.

American journal of physiology. Heart and circulatory physiology, 315(6), H1851-H1860 (2018-09-15)

Chemerin and its G protein-coupled receptor [chemerin receptor 23 (ChemR23)] have been associated with endothelial dysfunction, inflammation, and insulin resistance. However, the role of chemerin on insulin signaling in the vasculature is still unknown. We aimed to determine whether chemerin

Isolation Methods for Human CD34 Subsets Using Fluorescent and Magnetic Activated Cell Sorting: an In Vivo Comparative Study.

Himi Tripathi et al.

Stem cell reviews and reports, 16(2), 413-423 (2020-01-19)

Acute myocardial infarction (AMI) and resulting cardiac damage and heart failure are leading causes of morbidity and mortality worldwide. Multiple studies have examined the utility of CD34+ cells for the treatment of acute and ischemic heart disease. However, the optimal

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