expression extending from AEG
syndrome to absence of ocular involvement. Am. J.
Med. Genet. 143A: 289-291 (2007).
BKR,PHC 04/08-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich
S A. 103(45):16788-16793
Govoni KE (2006) Am J Physiol Endocrinol Metab. 291(1):E128-E136.
Vance KW (2005) Cancer Res. 65(6):2260-2268.
Important Note: During shipment, small volumes of
(1989) J Biol Chem. 264:8185; Kopito RR et al (1985) Nature 316:234;
Raida M et al (1989) BBA 980:291; Lux SE et al (1989) PNAS 86:9089; Tanner MJ et al
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Important Note
and Chudzik-Kozłowska, J., Acta
Alimentaria, 43(2), 193-291 (2014).
5. Cole, L.A. et al., Clin. Chem., 45(12), 2109-2119
(1999).
6. Figini, M., and Canevari, S., “Isolation of Human
Monoclonal
times, 300 µl per well, using
PBS with 0.05% TWEEN 20.
6. Prepare a working solution of the streptavidin-HRP
polymer conjugate by diluting 1:200 in PBS with
0.05% TWEEN 20. To assess background from
non-specific
.K. et al. (1988) J. Clin. Invest. 71:9.
11. Bottenstein, J.E. et al. (1988) J. Neurosci. Res. 20:291.
12. Suzumura, A. and Slerberg, D.H. (1989) Brain Res. 480:51.
13. Dubois-Dalcq, M. et al. (1990)
14618
(2010).
4. Szymkiewicz, A., and Chudzik-Kozłowska, J., Acta
Alimentaria, 43(2), 193-291 (2014).
5. Rosenqvist, E. et al., “Determination of Antibody
Responses to Meningococcal Antigens
Component Amount
DMEM/F12, with L-glutamine (Millipore Cat. No. DF-041-B) 291 mL
N-Base medium (Millipore Cat. No. N014-B) 291 mL
N21 medium supplement (Millipore Cat. No. SCM081) 10 mL
Neuro2 medium
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
mice. Neuron, 38, 291-303
(2003).
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Co. LLC.
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2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
tubule of mouse kidney; Ul tra struc tur al cytochemistry by a
new technique. J Histochem Cytochem 14:291, 1966.
3. Hanker JS, Yates PE, Metz CB, Rustioni A: A new specific sensitive and non-
carcinogenic
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
nm on a multiwell plate reader.
6. If you cannot read the plate immediately, the
reaction may be stopped by the addition of 50 µL
of 3 M HCl or 3 M H2SO4 per 200 µL of reaction
apoptosis and NF-kappa-B nuclear
translocation and localizes to mitochondria. FEBS
Lett., 473, 285-291 (2000).
2. Oberst, A. et al., Catalytic activity of the caspase-8-
FLIP(L) complex inhibits RIPK3
twice
with PBS with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG Phosphine Solution.
(200 µL/1.5 × 106 cells).
Notes:
• For labeling cytosolic proteins
twice
with PBS with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG Phosphine Solution.
(200 µL/1.5 × 106 cells).
Notes:
• For labeling cytosolic proteins
twice
with PBS with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG Phosphine Solution.
(200 µL/1.5 × 106 cells).
Notes:
• For labeling cytosolic proteins
twice
with PBS, with a centrifugation after each wash.
6. Incubate the cells overnight at 2–8 °C in the
250 µM FLAG-Phosphine Solution.
(200 µL/1.5 × 106 cells).
Lysis of Cells with RIPA Buffer