immediately after incubation.
9. Centrifuge the tubes at 2,000 rpm at 0-4 °C for
10 minutes.
10. Read the absorbance of the supernatant of each
tube at 415 nm.
11. Calculate the hemolytic
immediately after incubation.
9. Centrifuge the tubes at 2,000 rpm at 0-4 °C for
10 minutes.
10. Read the absorbance of the supernatant of each
tube at 415 nm.
11. Calculate the hemolytic
Cytokine QC Level Expected Range Units Cytokine QC Level Expected Range Units
sCD40L Control 1 200 – 415 pg/mL IL-1β Control 1 42 – 87 pg/mL
Control 2 849 – 1764 pg/mL
(2000).
9. Halayko AJ., et al., Am J Physiol., 276, L197-
206 (1999).
10. Landerholm TE., et al., Development, 126,
2053-62 (1999).
11. Wang C., et al., Tissue Eng Part A, 19, 415-25
(2013
pharmacology of the
orphanin FQ/nociceptin peptide and receptor family. Pharmacol. Rev. 53: 381-415.
For research use only; not for use as a diagnostic.
Unless otherwise stated in our
conditions and
200 ng/lane under reducing conditions as determined
by indirect immunoblotting.
References
1. Zsebo, K.M., et al., Cell, 63, 195 (1990).
2. Nocka, K., et al., EMBO J., 9, 3287 (1990
T., and Vale, R. (Eds.),
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415 (1981).
10 Carter, R., et al., Histopathology, 17, 301 (1990).
min),
then add 150 µl of the Stop solution to stop the HRP-substrate reaction. Read the plate at 415
nm in a plate reader.
* Alternatively, any other peroxidase substrate can be used (chromogenic or
Expr. Purif., 38(1),
108-115 (2004).
6. Zdanov, A.S. et al., Russ. J. Bioorg. Chem.,
29(5), 415-418 (2003).
Notice
We provide information and advice to our customers
on application technologies
photoreceptors, glia and extracellular space in drone retina. J. A.
Coles, R. K. Orkand, J. Physiol. 362, 415 (1985).
17 Quantitative relation of twitch and tonic tensions to intracellular Na+ activity in cardiac
Broder, Proc. Natl. Acad. Sci.
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Yarochoan, R., et. al., Science 245, 412-415 (1989)
Sigma warrants that its products conform to the information contained in this and other Sigma
reactivos y accesorios:
MQuantTM Test Molibdeno, art. 110049,
intervalo de medida 5 - 250 mg/l de Mo (8 - 415 mg/l de MoO42-)
MQuantTM Test Nitritos, art. 110057,
intervalo de medida 0,5 - 10 mg/l de NO2- (0,15
Add 20 µL of NaOH to each well.
9. Reading the plate
Read the plate in a fluorometer using the excitation wavelength of 375 nm and an emission wavelength
of 415 nm. Alternatively, excitation and
Environment, 23(4),
415-422 (2000).
7. Nakajima, T. et al., Cell Transplant., 10(4-5),
423-427 (2001).
8. Zhou, J. et al., Anal. Chem., 74(16), 4007-4010
(2002).
9. Wagschal, K. et al.
, Vesicular restriction of synaptobrevin
suggests a role for calcium in membrane fusion.
Nature, 415, 646-650 (2002).
8. Gincel, D., and Shoshan-Barmatz, V., The synaptic
vesicle protein Synaptophysin