200-540-9 | Sigma-Aldrich

Convenient Media Solutions for Food and Water

Mold 540 15 to 25 N/A 65009-20 SimPlate® 84 Well Plates plates 20 N/A 1825 15 to 25 63004-200 SimPlate® Supplement M vials 200 N/A 1080 2 to 8 63005-200 SimPlate® Supplement V vials 200 N/A 1080

Cathepsin B Detection Kit

. 15. If cells are to be stained with AO (Section 9) instead of Cathepsin B fluorogenic substrate, pipette the AO reagent stock 1:2000 to 1:200 (0.05 – 0.5% v/v) into the final cell suspension. (Because

Product Information Sheet - CS0370

480 nm excitation/540 nm emission lengths requires a wash step with PBS to remove any excess AO to reduce excessive brightness. 9. Wash procedure: • Gently pellet cells at 200 X g for 3-8 minutes

Product Information Sheet - CS0360

480 nm excitation/540 nm emission lengths requires a wash step with PBS to remove any excess AO to reduce excessive brightness. 9. Wash procedure: • Gently pellet cells at 200 X g for 3-8 minutes

Product Information Sheet - CS0350

480 nm excitation/540 nm emission lengths requires a wash step with PBS to remove any excess AO to reduce excessive brightness. 9. Wash procedure: • Gently pellet cells at 200 X g for 3-8 minutes

Purification of Process Solutions with Millistak+ A Series Filters -- A simple and efficient alternative to powdered carbon

1.5 0 200 400 600 800 Volume, ml R ed uc tio n in A bs or ba nc e 2430 LMH 540 LMH �� !�� $�� 9�� "� 9��

Poster: Montage PCRµ96 and SEQ96: A Total Solution for Sequencing PCR-derived Templates

Displaying Size Phred-30 (Phred 20 > 300) (Distance from Primer) Primer Plus 1 1/4 540 + 33 100% 2.1 75% 1/8 540 + 31 100% 1.2 96% 1/16 555 + 4 100% 1.0 100% 1/32 552 + 15 100% 1.8 79%

Product Information Sheet - A3759

para- aminobenzoic acid (PABA): a quantitative test of liver function. Clin. Biochem., 28(5), 531-540 (1995). 3. Chan, K., et al., Direct and simultaneous high- performance liquid chromatographic

BrdU Cell Proliferation Assay 200 Tests

-well microplate reader with 450-540 nm or 450-595 nm dual wavelength or 450 nm single wavelength filter. 8. Tissue culture microplate (96 well culture dish) 9. Sterile reagent troughs 10.

MOUSE ANTI-HUMAN MEROSIN (M-CHAIN)

Disord. 7: 211-216. 7. Noam, I. et al. (1997) Hum Genet. 99: 535-540. 8. Noam, I. et al. (1997) Neuromusc. Disord. 7: 176-179. 9. Tan, E. et al. (1997)

Product Information Sheet - CKH200A

times ê Add 200 µl Substrate Solution to each well. Incubate 20 min. RT ê Add 50 µl Stop Solution to each well. Read at 450 nm within 30 min.

MOUSE ANTI-HUMAN LAMININ (α5-CHAIN)

(1995) Neuromusc. Disord. 5(4): 307-316. 9. Taylor, J. et al. ( 1997) Neuromusc. Disord. 7: 211-216. 10. Noam, I. et al. (1997) Hum Genet. 99: 535-540. 11. Noam, I. et al. (1997) Neuromusc. Disord

Data Sheet - E8777

Hek3 Product Description Recombinant Mouse EphA8/Fc Chimera consists of amino acid residues 1-540 of the extracellular domain of mouse EphA81 fused by means of a polypeptide linker to the Fc portion

QCM™ Endothelial Cell Invasion Assay (24 well, colorimetric)

room temperature on an orbital shaker. 9. Transfer 100 µL of the solution to the included 96-well Stain Quantitation Plate and read absorbance at 540- 570 nm. 5

Data Sheet-ecm531

Materials Required (Not supplied) • Multi-channel or repeating pipettes • Microplate reader (540-570 nm detection) or spectrophotometer • CO2 incubator appropriate for subject cells • Hemacytometer

Data Sheet-ecm540

microscope for counting cells. • Trypan Blue or equivalent viability stain. • Microplate reader (540-570 nm detection) or spectrophotometer.

Product Information Sheet - N9389

Microbiology (Washington, D.C.), pp. 99-109 (1986). 4. Wei, J.S. et al., Clin. Chem., 39(3), 540-543 (1993). 5. Gioria, R. et al., Plant Pathology, 51, 127-133 (2002). 6. Owen, M.A.G. et

Product Information Sheet - MAK320

reduction of resazurin to the fluorescent dye resorufin (ex = 540 nm/em = 590 nm). Components The kit is sufficient for 200 fluorometric reactions D2HG Standard 100 l 10 mM 2-Hydroxyglutarate

Kits and Reagents for Metabolic and Dietary Research

m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to triglyceride concentration of the sample. Many

MAK407 - Technical Bulletin

and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm. The assay is simple, sensitive and high-throughput adaptable and can detect as low as 5 U of

Data Sheet - 90699

concentration)

Data Sheet - 89827

concentration)

Fluorescent IEF-Marker

pI 7.6 (three different concentration) 420 440 460 480 500 520 540 560 580 nm 495nm 17963 Fluorescent

Data Sheet - I9284

stained and scored for cytopathic effect by measurement of optical densities in a microplate reader at 540 nm. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody

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