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2010-15-3
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Kiwi EL自动过滤器完整性测试系统
Kiwi™ EL
仪器的气源接口和过滤器接口都是快接式的，因
此安装特别迅速。
3
与过滤器完整性测试相关的一些重要法规要求
“除菌过滤器使用后，应对其进行完整性测试，
并记录测试结果。测试方法可以是泡点、扩散流或压力保持。”
— SFDA指引，2010年
过滤器完整性测试是无菌药品生产和质量管理中的关键步骤，
世界各地的监管机构均要求对除菌过滤器进行完整性测试
Battery Test Report - 109194
G.W. GDM-391 Nov. 10, 2009 Nov. 09, 2010
Electronic Balance SHINKO HJ-33KE Nov. 16, 2009 Nov. 15, 2010
Climatic and T/H
Chamber ESPEC MZH-11H May 24, 2010
May 23, 2011
T &
Battery Test Report - 109208, 109191, 117103
G.W. GDM-391 Nov. 10, 2009 Nov. 09, 2010
Electronic Balance SHINKO HJ-33KE Nov. 16, 2009 Nov. 15, 2010
Climatic and T/H
Chamber ESPEC MZH-11H May 24, 2010
May 23, 2011
T &
CHOZN Platform
weeks
© 2010 SAFC Business confidential
15
The Benefits of Creating “Minipools”
SCC of the top pools
Media/feed/process
HEPES Sodium Salt Specification Comparison
sial.com/author/life-science/core-bioreagents/biological-buffers/hepes-sodium-salt.html (3 of 3) [8/13/2010 11:15:44 AM]
http://www.sigmaaldrich.com/catalog/search/ProductDetail?ProdNo=H3784&Brand
Cell Signaling Buffer and Detection Kit
techserv_dd@millipore.com
48-602 Rev. 20-SEP-2010 Millipore
Rho-associated Kinase (ROCK) Activity Assay
5 -4 -3 -2 -1 0 1 2 3 4
0
20
40
60
80
100
DMPK
ROCK I
ROCK II
Y27632 Log [uM]
Pe
rc
en
ta
ge
In
hi
bi
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Product Information Sheet - PKH67GL
548-558 (2010).
14. Givan, A.L., Immunol Invest., 36, 563-580 (2007).
15. Schwaab, T. et al., Immunol Invest., 36, 649-664 (2007).
16. Barth, R.J. et al., Clin. Cancer Res., 15, 5548-5556 (2010).
17. Kusumbe
Product Information Sheet - MINI67
548-558 (2010).
14. Givan, A.L., Immunol Invest., 36, 563-580 (2007).
15. Schwaab, T. et al., Immunol Invest., 36, 649-664 (2007).
16. Barth, R.J. et al., Clin. Cancer Res., 15, 5548-5556 (2010).
17. Kusumbe
Product Information Sheet - MIDI67
548-558 (2010).
14. Givan, A.L., Immunol Invest., 36, 563-580 (2007).
15. Schwaab, T. et al., Immunol Invest., 36, 649-664 (2007).
16. Barth, R.J. et al., Clin. Cancer Res., 15, 5548-5556 (2010).
17. Kusumbe
Phospho-PRAS40 (Thr246) MAPmates
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification buffer.
Page 6 Millipore 46-681 Rev. 08-JUN-2010
EMBRYOID BODY (EB) FORMATION MEDIUM
www.millipore.com
02-23-2010/SCM018/AN/JM
FORMATION OF
EMBRYOID BODY:
Mouse ES cells should be grown in proper cell culture conditions for at least 3 passages
before attempting to
MESENCHYMAL STEM CELL EXPANSION MEDIUM
pre-warmed to 37°C)
to the plate.
5. Transfer the dissociated cells to a 15 mL conical tube.
6. Centrifuge the tube at 300 xg for 2-3 minutes to pellet the cells.
7. Discard the supernatant
8. Apply 2
Phospho ERK/MAP Kinase 1/2 (Thr185/Tyr187) MAPmates
shaking;
dark
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification Buffer.
Page 6 of 8 Millipore 46-602
CARDIOMYOCYTE DIFFERENTIATION MEDIUM
Medium (pre-warmed to 37°C) to the plate.
3. Replace with fresh Cardiomyocyte Differentiation Medium (pre-warmed to 37°C) every
2 to 3 days for 12-15 days.
STORAGE/HANDLING:
For long term
Total PTEN MAPmates
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification buffer.
Page 5 Millipore 46-678 Rev. 08-JUN-2010
Data Sheet - SAB4200883
2, 2177-97
(2010).
2. Bergdoll MS., Enterotoxins. In: Staphylococci and
Staphylococcal Infections, Easmon CSF. and
Adlam C. (eds), Academic Press, London 559-598
(1983).
3. Miron N. and Miron
Quantitative Alkaline Phosphatase ES Characterization Kit
first 3 columns (A1-3 through H1-3) of the 96-well plate which should be reserved to run the 8-
point alkaline phosphatase standard curve.
Product Information Sheet - PKH26PCL
(2009).
14. Wygrecka, M. et al., Blood 113: 5588-5598
(2009).
15. Smith, A.R. et al., Am J Resp Crit Care Med,
181: A1289 (2010).
16. Martin, W.J. et al., Arthr Rheum, DOI
10.1002/art.30245 (
Data Sheet - SAB4200859
2177-97 (2010).
2. Bergdoll MS., Enterotoxins. In: Staphylococci and
Staphylococcal Infections, Easmon CSF. and
Adlam C. (eds), Academic Press, London
559-598 (1983).
3. Miron N. and
MILLIPLEX MAP Phospho IGF1R (pan Tyr) MAPmates
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 L Amplification Buffer.
46-647 Rev. 26-Jan-2010 Millipore Page 5
13. Incubate
Red Fluorescent Cell Linker Kits for General Cell Membrane Labeling
Immunol., 71, 496-504
(2010).
14. Bantly, A.D., et al., Immunol. Invest., 36, 587-606 (2007).
15. Schwaab, T. et al., Immunol Invest., 36, 649-664 (2007).
16. Cicalese, A., et al., Cell 138, 1083
Product Information Sheet - MIDI26
Immunol., 71, 496-504
(2010).
14. Bantly, A.D., et al., Immunol. Invest., 36, 587-606 (2007).
15. Schwaab, T. et al., Immunol Invest., 36, 649-664 (2007).
16. Cicalese, A., et al., Cell 138, 1083
Scepter 2.0 Cell Counter
University’s
Lewis-Sigler Institute for
Integrative Genomics
The Scientist, Dec. 2010.
Top Ten Innovations of 2010.
“Cell counting is normally
a very tedious process and
usually only provides minimal
MESENCHYMAL STEM CELL FREEZING MEDIUM (1X)
www.millipore.com
03-05-2010/SCM016/AN
e. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem
Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube.
IMPORTANT
ACCUMAX™
www.millipore.com
03-18-2010/SCR006/PM
Sterile Materials required:
ACCUMAX™ T25 culture flasks
DPBS (calcium and magnesium
free)
Centrifuge tubes (15-50 ml)
Culture medium
Cleaved PARP MAPmates
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification Buffer.
Page 5 of 7 Millipore 46-656 Rev. 03-AUG-2010
13. Incubate
Phospho-PTEN (Ser380) MAPmates
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification buffer.
Page 6 Millipore 46-679 Rev. 07-JUN-2010
Total PRAS40 MAPmates
shaking;
dark
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification buffer.
Page 5 of 7 Millipore 46-680
MILLIPLEX MAP Total p53 MAPmates
Incubate 15 min at
RT with shaking;
dark
DO NOT REMOVE SAPE and
add 25 µL Amplification buffer.
Page 5 of 7 Millipore 46-662 Rev. 17-JUN-2010
14. Incubate
1/40