21134

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Effect of acidic condition on the metabolic regulation of Escherichia coli and its phoB mutant.

Lolo Wal Marzan et al.

Archives of microbiology, 195(3), 161-171 (2013-01-01)

Effect of acidic condition on the fermentation characteristics was investigated by the continuous culture of Escherichia coli. In accordance with down-regulation of crp gene transcript level as well as up-regulation of arcA, the expressions of the TCA cycle genes were

Four products from Escherichia coli pseudogenes increase hydrogen production.

Mohd Zulkhairi Mohd Yusoff et al.

Biochemical and biophysical research communications, 439(4), 576-579 (2013-09-13)

Pseudogenes are considered to be nonfunctional genes that lack a physiological role. By screening 3985 Escherichia coli mutants using chemochromic membranes, we found four pseudogenes involved in hydrogen metabolism. Knockouts of pseudogenes ydfW and ypdJ had a defective hydrogen phenotype

Microbiology. An enzymatic route to H2 storage.

Inês A C Pereira

Science (New York, N.Y.), 342(6164), 1329-1330 (2013-12-18)

Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products.

Lydia Beyer et al.

Journal of bacteriology, 195(7), 1428-1435 (2013-01-22)

Enterobacteria such as Escherichia coli generate formate, lactate, acetate, and succinate as major acidic fermentation products. Accumulation of these products in the cytoplasm would lead to uncoupling of the membrane potential, and therefore they must be either metabolized rapidly or

Bio-inspired catalytic imine reduction by rhodium complexes with tethered Hantzsch pyridinium groups: evidence for direct hydride transfer from dihydropyridine to metal-activated substrate.

Alex McSkimming et al.

Angewandte Chemie (International ed. in English), 52(12), 3411-3416 (2013-02-27)

Photocatalytic reduction of nitrate using titanium dioxide for regeneration of ion exchange brine.

Ting Yang et al.

Water research, 47(3), 1299-1307 (2013-01-02)

Nitrate is often removed from groundwater by ion exchange (IX) before its use as drinking water. Accumulation of nitrate in IX brine reduces the efficiency of IX regeneration and the useful life of the regeneration brine. For the first time

Density functional theory study on the adsorption and decomposition of the formic acid catalyzed by highly active mushroom-like Au@Pd@Pt tri-metallic nanoparticles.

Sai Duan et al.

Physical chemistry chemical physics : PCCP, 15(13), 4625-4633 (2013-02-21)

Local structures and adsorption energies of a formic acid molecule and its decomposed intermediates (H, O, OH, CO, HCOO, and COOH) on highly electrocatalytically active mushroom-like Au-core@Pd-shell@Pt-cluster nanoparticles with two atomic layers of the Pd shell and stoichiometric Pt coverage

GC-MS, LC-MS(n), LC-high resolution-MS(n), and NMR studies on the metabolism and toxicological detection of mesembrine and mesembrenone, the main alkaloids of the legal high "Kanna" isolated from Sceletium tortuosum.

Golo M J Meyer et al.

Analytical and bioanalytical chemistry, 407(3), 761-778 (2014-09-23)

Mesembrine and mesembrenone are the main alkaloids of Sceletium tortuosum, a plant species that was used for sedation and analgesia by the KhoiSan, previously known as Hottentots, a tribe in South Africa. After fermentation, the obtained preparation called "Kanna" or

Direct and reversible hydrogenation of CO2 to formate by a bacterial carbon dioxide reductase.

K Schuchmann et al.

Science (New York, N.Y.), 342(6164), 1382-1385 (2013-12-18)

Storage and transportation of hydrogen is a major obstacle for its use as a fuel. An increasingly considered alternative for the direct handling of hydrogen is to use carbon dioxide (CO2) as an intermediate storage material. However, CO2 is thermodynamically

Sodium phenylbutyrate enhances astrocytic neurotrophin synthesis via protein kinase C (PKC)-mediated activation of cAMP-response element-binding protein (CREB): implications for Alzheimer disease therapy.

Grant T Corbett et al.

The Journal of biological chemistry, 288(12), 8299-8312 (2013-02-14)

Neurotrophins, such as brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), are believed to be genuine molecular mediators of neuronal growth and homeostatic synapse activity. However, levels of these neurotrophic factors decrease in different brain regions of patients with Alzheimer disease

Bionanowhiskers from jute: preparation and characterization.

Nibedita Kasyapi et al.

Carbohydrate polymers, 92(2), 1116-1123 (2013-02-13)

Bionanowhiskers were extracted from jute by acid hydrolysis. At first cellulose microfibrils were formed by alkali treatment. Addition of an acid to the microfibrils triggered the formation of cellulose nanowhiskers. These were characterized by using different techniques viz. Fourier transform

Immunohistochemical characterization of novel monoclonal antibodies against the N-terminus of amyloid β-peptide.

Nicolaas A Verwey et al.

Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis, 20(3), 179-187 (2013-07-09)

Abstract Amyloid β-peptide (Aβ) is a key molecule in Alzheimer's disease (AD). Reliable immunohistochemical (IHC) methods to detect Aβ and Aβ-associated factors (AAF) in brain specimens are needed to determine their role in AD pathophysiology. Formic acid (FA) pre-treatment, which

Direct analysis of formate in human plasma, serum and whole blood by in-line coupling of microdialysis to capillary electrophoresis for rapid diagnosis of methanol poisoning.

Pavel Kubáň et al.

Analytica chimica acta, 768, 82-89 (2013-03-12)

A simple method using direct injection of human blood samples and quantitative analysis of formate was developed for rapid diagnosis of methanol poisoning. A sample pretreatment device including a 500Da molecular weight cut-off dialysis membrane was in-line coupled to capillary

Effects of dietary supplementation with two potassium formate sources on performance of 8- to 22-kg pigs.

J K Htoo et al.

Journal of animal science, 90 Suppl 4, 346-349 (2013-02-13)

Dietary inclusion of salts of organic acids may modulate intestinal microbiota and enhance performance of pigs. Published data on the effects of potassium formate (KF; pH 6.0-8.5) on pig performance are scarce compared with that of potassium diformate (KDF; pH

Monoclonal antibodies selective for α-synuclein oligomers/protofibrils recognize brain pathology in Lewy body disorders and α-synuclein transgenic mice with the disease-causing A30P mutation.

Therese Fagerqvist et al.

Journal of neurochemistry, 126(1), 131-144 (2013-02-01)

Inclusions of intraneuronal alpha-synuclein (α-synuclein) can be detected in brains of patients with Parkinson's disease and dementia with Lewy bodies. The aggregation of α-synuclein is a central feature of the disease pathogenesis. Among the different α-synuclein species, large oligomers/protofibrils have

Changes in pH and organic acids in mucilage of Eriophorum angustifolium roots after exposure to elevated concentrations of toxic elements.

M Tariq Javed et al.

Environmental science and pollution research international, 20(3), 1876-1880 (2013-01-01)

The presence of Eriophorum angustifolium in mine tailings of pyrite maintains a neutral pH, despite weathering, thus lowering the release of toxic elements into acid mine drainage water. We investigated if the presence of slightly elevated levels of free toxic

Optimal design and experimental validation of a simulated moving bed chromatography for continuous recovery of formic acid in a model mixture of three organic acids from Actinobacillus bacteria fermentation.

Chanhun Park et al.

Journal of chromatography. A, 1365, 106-114 (2014-09-23)

The economically-efficient separation of formic acid from acetic acid and succinic acid has been a key issue in the production of formic acid with the Actinobacillus bacteria fermentation. To address this issue, an optimal three-zone simulated moving bed (SMB) chromatography

Determination of buprenorphine, fentanyl and LSD in whole blood by UPLC-MS-MS.

Thomas Berg et al.

Journal of analytical toxicology, 37(3), 159-165 (2013-02-21)

A sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method has been developed and validated for the quantification of buprenorphine, fentanyl and lysergic acid diethylamide (LSD) in whole blood. Sample preparation was performed by liquid-liquid extraction (LLE) with methyl tert-butyl ether.

An efficient CoAuPd/C catalyst for hydrogen generation from formic acid at room temperature.

Zhi-Li Wang et al.

Angewandte Chemie (International ed. in English), 52(16), 4406-4409 (2013-03-21)

Capillary electrophoresis with contactless conductometric detection for rapid screening of formate in blood serum after methanol intoxication.

Petr Kubáň et al.

Journal of chromatography. A, 1281, 142-147 (2013-02-20)

A new method for rapid, direct determination of formate in blood serum samples by capillary electrophoresis with contactless conductometric detection is presented. A selective separation of formate was achieved in approximately 1 min using an electrolyte system comprising 10 mM

Deoxy-liquefaction of switchgrass in supercritical water with calcium formate as an in-situ hydrogen donor.

Hema Ramsurn et al.

Bioresource technology, 143, 575-583 (2013-07-10)

Switchgrass was liquefied in supercritical water (SCW) using Ca(HCOO)2 as an in-situ source of hydrogen to enhance deoxygenation and the quality of the biocrude obtained. In SCW, Ca(HCOO)2 produces hydrogen via decomposition and hydrolysis reactions, and simultaneously switchgrass hydrolyzes to

Acid-catalyzed hydrothermal severity on the fractionation of agricultural residues for xylose-rich hydrolyzates.

Ji Ye Lee et al.

Bioresource technology, 132, 84-90 (2013-02-12)

The objective of this work was to investigate the feasibility of acid-catalyzed hydrothermal fractionation for maximum solubilization of the hemicellulosic portion of three agricultural residues. The fractionation conditions converted into combined severity factor (CS) in the range of 1.2-2.9. The

Chemical shift powder spectra enhanced by multiple-contact cross-polarization under slow magic-angle spinning.

Jésus Raya et al.

Journal of magnetic resonance (San Diego, Calif. : 1997), 227, 93-102 (2013-01-15)

A simple multiple-contact cross-polarization (CP) scheme is applied to a powder sample of ferrocene and β-calcium formate under static and magic-angle spinning (MAS) conditions. The method is described analytically through the density matrix formalism. We show that multiple equilibrations-re-equilibrations with

Acidification due to microbial dechlorination near a trichloroethene DNAPL is overcome with pH buffer or formate as electron donor: experimental demonstration in diffusion-cells.

Jo Philips et al.

Journal of contaminant hydrology, 147, 25-33 (2013-03-19)

Acidification due to microbial dechlorination of trichloroethene (TCE) can limit the bio-enhanced dissolution of TCE dense non-aqueous phase liquid (DNAPL). This study related the dissolution enhancement of a TCE DNAPL to the pH buffer capacity of the medium and the

Improvement of ethanol productivity and energy efficiency by degradation of inhibitors using recombinant Zymomonas mobilis (pHW20a-fdh).

Hong-Wei Dong et al.

Biotechnology and bioengineering, 110(9), 2395-2404 (2013-03-12)

Toxic compounds, such as formic acid, furfural, and hydroxymethylfurfural (HMF) generated during pretreatment of corn stover (CS) at high temperature and low pH, inhibit growth of Zymomonas mobilis and lower the conversion efficiency of CS to biofuel and other products.

Determination of phenylephrine in human plasma using ultra-performance liquid chromatography-tandem mass spectrometry.

Sheng Feng et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 915-916, 28-32 (2013-01-15)

This paper described a sensitive and rapid method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) for the determination of phenylephrine in human plasma. Plasma samples were pre-purified by solid-phase extraction (SPE). The chromatographic separation was achieved

New sulfate-reducing bacteria isolated from Buryatian alkaline brackish lakes: description of Desulfonatronum buryatense sp. nov.

Yana Ryzhmanova et al.

Extremophiles : life under extreme conditions, 17(5), 851-859 (2013-07-25)

New strains of sulfate-reducing bacteria were isolated from two alkaline brackish lakes located in the Siberian region of Russia, namely in the Southern Transbaikalia, Buriatia. The article presents data describing morphology, physiology, and biochemical characteristics of the isolated strains. These

The Fantastic Four: A plug 'n' play set of optimal control pulses for enhancing NMR spectroscopy.

Manoj Nimbalkar et al.

Journal of magnetic resonance (San Diego, Calif. : 1997), 228, 16-31 (2013-01-22)

We present highly robust, optimal control-based shaped pulses designed to replace all 90° and 180° hard pulses in a given pulse sequence for improved performance. Special attention was devoted to ensuring that the pulses can be simply substituted in a

Determination of organochlorines, polychlorinated biphenyls and polybrominated diphenyl ethers in human hair: estimation of external and internal exposure.

Dasheng Lu et al.

Chemosphere, 114, 327-336 (2014-08-13)

A novel method was developed for the analysis of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) in human hair samples. External contaminants of hair were extracted with acetone under sonication, while washed hair was further hydrolyzed

Deletion of Mthfd1l causes embryonic lethality and neural tube and craniofacial defects in mice.

Jessica Momb et al.

Proceedings of the National Academy of Sciences of the United States of America, 110(2), 549-554 (2012-12-26)

Maternal supplementation with folic acid is known to reduce the incidence of neural tube defects (NTDs) by as much as 70%. Despite the strong clinical link between folate and NTDs, the biochemical mechanisms through which folic acid acts during neural

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