Lindquist, S.L., Nat. Rev.
Cancer, 5, 761-72 (2005).
3. Tas, F. et al., Asian Pac. J. Cancer Prev., 18,
599-601 (2017).
4. Morán Luengo, T. et al., Mol. Cell., 70, 545-
552.e9 (2018).
disturb the pellet. Discard the supernatant.
6. Repeat steps 3-5 two additional times.
Notes: Larger quantities of plant tissue
(200-250 mg) or tissues known for high phenolics
and tannin content may
disturb the pellet. Discard the supernatant.
6. Repeat steps 3–5 two additional times.
Notes: Larger quantities of plant tissue
(200–250 mg) or tissues known for high phenolics
and tannin content may
disturb the pellet. Discard the supernatant.
6. Repeat steps 3–5 two additional times.
Notes: Larger quantities of plant tissue
(200–250 mg) or tissues known for high phenolics
and tannin content may
acid 122.0 °C (±0.3 °C) (Thermodynamic Mode) 5 g
42183 Melting point standard 182-184 °C 4-Methoxybenzoic acid 183.2 °C (±0.3 °C) (Thermodynamic Mode)
250 mg
1 g
41019 Melting point standard 235
disturb the pellet. Discard the supernatant.
6. Repeat steps 3–5 two additional times.
Notes: Larger quantities of plant tissue
(200–250 mg) or tissues known for high phenolics
and tannin content may
Standard: Prepare 250 L of 80 M
sodium cholate by mixing 20 L of Standard and
230 L of ultrapure water.
2. Transfer 20 L of sample to each of the three
wells.
Features
Feature Map Position
Bgl II compatible end 1-5
MAT tag 6-26
T1/T2 terminator 83-453
Verification Primer-T7R 161-182
β-lactamase (ampr) 545-1405
pBR322 ori 1617-1736
f1 ori 2400-2863
lacI 3541-4623
tested.
5. Incubate “test” and “control” tubes at 37°C for 30-60
minutes. Exact incubation time may require optimization
for different cell types.
6. Centrifuge all tubes at 250 x g for 5 minutes
5
Reagents Preparation
1. Autophagy Reagent A: This material is supplied in a lyophilized vial. Prior to use,
reconstitute the contents of the vial in 250 µL Milli-Q or deionized
PBS.
Centrifuge the sample at 250 x g for 5 minutes. Discard the supernatant and resuspend the
cell pellet in 40 mL of ice-cold PBS. Centrifuge the suspension at 250 x g as before. Repeat.
Pour off
PBS.
Centrifuge the sample at 250 x g for 5 minutes. Discard the supernatant and resuspend the
cell pellet in 40 mL of ice-cold PBS. Centrifuge the suspension at 250 x g as before. Repeat.
Pour off
PBS.
Centrifuge the sample at 250 x g for 5 minutes. Discard the supernatant and resuspend the
cell pellet in 40 mL of ice-cold PBS. Centrifuge the suspension at 250 x g as before. Repeat.
Pour off
PBS.
Centrifuge the sample at 250 x g for 5 minutes. Discard the supernatant and resuspend the
cell pellet in 40 mL of ice-cold PBS. Centrifuge the suspension at 250 x g as before. Repeat.
Pour off
PBS.
Centrifuge the sample at 250 x g for 5 minutes. Discard the supernatant and resuspend the
cell pellet in 40 mL of ice-cold PBS. Centrifuge the suspension at 250 x g as before. Repeat.
Pour off
plasmid, pKD-Bcl3-v1 H RNAi 5 µg 62-133 $349
siRNA plasmid, pKD-Bcl3-v4 RNAi 5 µg 62-233 $349
Bcl6
Anti-Bcl-6, clone BL6.02 (PG-B6p) H R Sh Rb B Po IH(P) M IgG1κ 250 µL MAB4618 $210
plasmid, pKD-Par-1B-v3 H RNAi 5 mg 62-233
siRNA plasmid, pKD-Par-1B-v4 H RNAi 5 mg 62-334
Paxillin
Monoclonal Antibody
Anti-Paxillin 5H11 H M R B Av WB IP IC Pur M IgG1 250 µg 05-417
Paxillin 349