A Agarose (right, Product Code: P 2545).
Figure 3. EZviewTM Red Protein A Affinity Gel (Product Code: P 6486)
and standard protein A agarose (Product Code: P 2545) recovered a
similar amount of IgG
Protein A-Agarose
beads (30µl packed beads) (Sigma Product No.
P 2545).
5. Gently rock reaction mixture at 4 °C for 1 hour.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
slurry of Protein A-Agarose
beads (50 µl packed beads) (Product No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
Protein A-Agarose
beads (50 µl packed beads) (Sigma Product No.
P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
slurry of Protein A-Agarose
beads (50 µl packed beads) (Product No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
Protein A-Agarose
beads (50 µl packed beads) (Sigma Product
No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
Protein A-Agarose
beads (50 :l packed beads) (Sigma Product
No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
slurry of Protein A-Agarose
beads (50 µl packed beads) (Product No. P 2545).
5. Gently rock reaction mixture at 2-8 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
slurry of Protein A-Agarose
beads (50 µl packed beads) (Product No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
Protein A-Agarose
beads (50 :l packed beads) (Sigma Product
No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
slurry of Protein A-Agarose
beads (50 µl packed beads) (Product No. P 2545).
5. Gently rock reaction mixture at 4 °C for 2 hours.
6. Collect the agarose beads by pulsing (5 seconds in
the microcentrifuge
F
Adenine
(6-Aminopurine; Vitamin B4)
CAS No. 73-24-5
C5H5N5 FW 135.1
Plant cell culture, tested, minimum99%
R: 22 S: 22-26-36
25 g
100 g
500 g
A 2545
F
Adenine sulfate salt
(6-Aminopurine hemisulfate
– 1165-1167 bp) and GCC-GCT (Ala – 1870-1872 bp))
and a single coding mutation AGG-GGG (Arg-Gly – 2545-2547 bp) in the rest of
the sequence with respect to the accession number (NM_001194).
ATGGACGCTAGAGGAGGTGGAGGAAGACCTGGAGAGAGCCCGGGAGCTACCCCTGCTCCTGGACCTCCA