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Hyung-Keun Ku et al.
Analytical biochemistry, 434(1), 178-180 (2012-12-04)
The Bradford assay is a simple method for protein quantitation, but variation in the results between proteins is a matter of concern. In this study, we compared and normalized quantitative values from two models for protein quantitation, where the residues
Simona Matrella et al.
Macromolecular bioscience, 15(7), 927-940 (2015-03-18)
Intrinsic antimicrobial thermoplastic A(BC)n copolymers (n = 1, 2, 4), where A was poly(ethylene glycol) (PEG), BC was a random chain of methylmethacrylate (MMA), and alkyl-aminoethyl methacrylate (AAEMA), were synthesized and the antimicrobial activity and hemolyticity were evaluated on plaques obtained by
Sophia Schmitt et al.
ACS nano, 9(4), 4219-4226 (2015-03-25)
The controlled synthesis of hierarchically functionalized core/multishell particles is highly desirable for applications in medicine, catalysis, and separation. Here, we describe the synthesis of hierarchically structured metal-organic framework multishells around magnetic core particles (magMOFs) via layer-by-layer (LbL) synthesis. The LbL
Ashleigh Willis et al.
Journal of neuroscience methods, 308, 1-5 (2018-07-24)
Primary neuronal cultures underpin diverse neuroscience experiments, including various protein analysis techniques, such as Western blotting, whereby protein extraction from cultured neurons is required. During immunoblotting experiments, we encountered problems due to a highly-abundant protein of 65-70 KDa present in
Nadine Dyballa et al.
Methods in molecular biology (Clifton, N.J.), 893, 47-59 (2012-06-06)
Proteins separated by two-dimensional gel electrophoresis can be visualized by in-gel detection using -different staining methods. Ideally, the dye should bind non-covalently to the protein following a linear response curve. Since protein concentrations in biological systems may vary by six
Shoji Notomi et al.
PloS one, 8(1), e53338-e53338 (2013-01-12)
Photoreceptor degeneration is the most critical cause of visual impairment in age-related macular degeneration (AMD). In neovascular form of AMD, severe photoreceptor loss develops with subretinal hemorrhage due to choroidal neovascularization (CNV), growth of abnormal blood vessels from choroidal circulation.
Lene K Vestby et al.
BMC veterinary research, 5, 43-43 (2009-11-26)
Biofilm has been shown to be one way for Salmonella to persist in the feed factory environment. Matrix components, such as fimbriae and cellulose, have been suggested to play an important role in the survival of Salmonella in the environment.
Sungwon Han et al.
Analytical and bioanalytical chemistry, 405(6), 1821-1831 (2012-09-25)
Gel electrophoresis is routinely used to separate and analyse macromolecules in biological systems. Although many of these electrophoretic techniques necessitate the denaturing of the analytes prior to their analysis, blue native polyacrylamide gel electrophoresis (BN-PAGE) permits the investigation of proteins/enzymes
Yüksel Totan et al.
The British journal of ophthalmology, 99(1), 75-80 (2014-08-06)
To evaluate the potential efficacy and safety of inner limiting membrane (ILM) staining with brilliant blue G (BBG) under air infusion. 63 patients, treated by one surgeon with macular hole or idiopathic epiretinal membrane were evaluated prospectively. BBG was applied
S Bag et al.
Organic & biomolecular chemistry, 13(6), 1763-1767 (2014-12-17)
Spectroscopic and in cellulo studies are here reported on the very first BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette where the luminol CL agent is covalently linked to the BODIPY energy-transfer acceptor in a molecular dyad. The efficiency of intramolecular CRET
Søren U Nielsen et al.
The Journal of general virology, 89(Pt 10), 2507-2517 (2008-09-18)
Hepatitis C virus (HCV) particles found in vivo are heterogeneous in density and size, but their detailed characterization has been restricted by the low titre of HCV in human serum. Previously, our group has found that HCV circulates in blood
Ronald Derking et al.
Cell reports, 35(1), 108933-108933 (2021-04-08)
Artificial glycan holes on recombinant Env-based vaccines occur when a potential N-linked glycosylation site (PNGS) is under-occupied, but not on their viral counterparts. Native-like SOSIP trimers, including clinical candidates, contain such holes in the glycan shield that induce strain-specific neutralizing
A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.
J J Sedmak et al.
Analytical biochemistry, 79(1-2), 544-552 (1977-05-01)
Sangjun Lee et al.
Carbohydrate polymers, 121, 284-294 (2015-02-11)
In this study, we synthesized dimer acid (DA) esters, having short to long alkyl chains, (DA-Cn) by the Diels-Alder reaction and subsequent esterification reaction of fatty acids that were prepared by the hydrolysis of waste vegetable oil. It was found
David E Pelayes et al.
Ophthalmic research, 48 Suppl 1, 21-25 (2012-08-29)
Brilliant blue G (BBG) is frequently used in chromovitrectomy to facilitate internal limiting membrane (ILM) peeling. A study was initiated to evaluate if heavy BBG is safe and effective in staining the ILM. We studied 30 eyes, 23 with idiopathic
Adam Aguirre et al.
Journal of cellular physiology, 228(2), 485-493 (2012-07-19)
Fas ligation via the ligand FasL activates the caspase-8/caspase-3-dependent extrinsic death pathway. In so-called type II cells, an additional mechanism involving tBid-mediated caspase-9 activation is required to efficiently trigger cell death. Other pathways linking FasL-Fas interaction to activation of the
Sheng Chen et al.
Critical care medicine, 41(12), e466-e474 (2013-08-22)
Brilliant blue G, a selective P2X7 receptor antagonist, exhibits neuroprotective properties. This study examined whether brilliant blue G treatment ameliorates early brain injury after experimental subarachnoid hemorrhage, specifically via inhibiting p38 mitogen-activated protein kinase-related proapoptotic pathways. Controlled in vivo laboratory
Veronica Zaga-Clavellina et al.
Reproductive biology and endocrinology : RB&E, 9, 13-13 (2011-01-27)
Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations of the extracellular matrix (ECM) homeostasis. The aim of this work was to evaluate the integral/functional response of the amnion (AMN) and choriodecidua (CHD) to
Song Wei et al.
Bioorganicheskaia khimiia, 38(5), 535-544 (2013-01-25)
A novel 4-(tetrahydro-2-furanmethoxy)-N-octadecyl-1,8-naphthalimide (4-TN1) blue emitting fluorophore was synthesized for solvent polarity probing and protein detection. The effect of various solvents on absorption and fluorescence spectra of 4-TNI was investigated. By comparison with other derivatives with heterocyclic electron-donating groups, 4-TNI
Joseph M Tuscano et al.
Cancer immunology, immunotherapy : CII, 60(6), 771-780 (2011-02-25)
Previous studies have shown that bispecific antibodies that target both CD20 and CD22 have in vivo lymphomacidal properties. We developed a CD20-CD22 bispecific antibody (Bs20x22) from anti-CD20 and the anti-CD22 monoclonal antibodies (mAb), rituximab and HB22.7, respectively. Bs20x22 was constructed
Chiara Rigato et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(34), 11559-11573 (2012-08-24)
Microglia are known to invade the mammalian spinal cord (SC) at an early embryonic stage. While the mechanisms underlying this early colonization of the nervous system are still unknown, we recently found that it is associated, at least partially, with
W.J. Shaphonov
Methods in Enzymology, 11, 184-184 (1967)
Jijun Zhao et al.
Arthritis and rheumatism, 65(12), 3176-3185 (2013-09-12)
The NLRP3 inflammasome plays key roles in inflammation and autoimmunity, and purinergic receptor P2X7 has been proposed to be upstream of NLRP3 activation. The aim of the present study, using murine models, was to investigate whether the P2X7 /NLRP3 inflammasome
P P Akalin et al.
Polish journal of veterinary sciences, 22(2), 271-278 (2019-07-04)
This study aimed to determine the levels of milk cell total protein (TP), reduced nicotinamide adenine dinucleotide phosphate (NADPH), total glutathione (tGSH), activities of glucose-6-phosphate dehydrogenase (G6PD) and glutathione peroxidase (GPx) in subclinical mastitic cows. Milk from each udder was
Hiroshi Enaida et al.
Retina (Philadelphia, Pa.), 26(6), 631-636 (2006-07-11)
To report the use of the dye brilliant blue G (BBG) for staining of the internal limiting membrane (ILM) during macular hole (MH) and epiretinal membrane (ERM) surgery. This study was designed as an interventional, noncomparative, prospective, clinical case series.
Y L Ooi et al.
Eye (London, England), 27(3), 425-431 (2012-12-01)
To compare the potential retinal toxicity of two commercially Brilliant blue G dyes (Brilliant Peel and Ocublue Plus) and Indocyanine green (ICG) at usual clinical concentration. Brilliant Peel 0.025% (n=9), Ocublue Plus 0.025% (n=9), and ICG 0.05% (n=9) were injected
Broc R Wenrich et al.
Analytical biochemistry, 428(2), 93-95 (2012-06-30)
The Bradford assay has been used reliably for decades to quantify protein in solution. The analyte is incubated in acidic solution of Coomassie Blue G-250 dye, during which reversible ionic and nonionic binding interactions form. Bradford assay color yields were
Kristen L Huber et al.
Protein expression and purification, 67(2), 139-147 (2009-05-22)
Peptide libraries have proven to be useful in applications such as substrate profiling, drug candidate screening and identifying protein-protein interaction partners. However, issues of fidelity, peptide length, and purity have been encountered when peptide libraries are chemically synthesized. Biochemically produced
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M M Bradford
Analytical biochemistry, 72, 248-254 (1976-05-07)
Hyo Jin Kang et al.
Biosensors & bioelectronics, 67, 413-418 (2014-09-15)
Caspase-3 is an apoptotic cysteine protease and its aberrancy is highly implicated to numerous diseases thereby rendering caspase-3 activity as an important disease marker. Most caspase-3 sensors are caspase-3 substrates of which the fluorescence signals are turned on upon catalytic
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