Germany), p. 23 (1983).
6. Rozengurt, E. and Heppel, L., Proc. Natl. Acad. Sci.
USA, 72, 4492 (1975).
7. Barnes, D. and Colowick, S., J. Cell. Physiol., 89,
633 (1976).
8. Diamond, I., et
Germany), p. 23 (1983).
6. Rozengurt, E. and Heppel, L., Proc. Natl. Acad.
Sci. USA, 72, 4492 (1975).
7. Barnes, D. and Colowick, S., J. Cell. Physiol.,
89, 633 (1976).
8. Diamond, I., et al
in Control of Animal and Plant
Growth, Grane, F.L., ed., Plenum Press (New York,
NY: 1988) pp. 295-302.
4. Wakasugi, N., et al., Proc. Natl. Acad. Sci. U.S.A.,
87, 8282-8286 (1990).
5. Oblong, J.E
glycerol.
Molecular mass: ∼53 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 224–302 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
glycerol.
Molecular mass: ∼53 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 224–302 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
glycerol.
Molecular mass: ∼53 kDa
Purity: ≥70% (SDS-PAGE, see Figure 1)
Specific Activity: 224–302 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
T. and Schildkraut, I. (1990) Nucl. Acids Res. 18, 3666.
6 Shimizu, Y. et al. (1983) Nature 302, 587-590.
7 Skoglund, C.M. et al. (1990) Gene 88, 1-5.
8 Rebase The Restriction Enzyme Database:
http
Thermocycler
d. Gel electrophoresis apparatus (vertical or horizontal)
e. Power Supply
f. 302 nm UV transilluminator, camera and film
Reagents
a 2.5 mM dNTP mix (2.5 mM of each nucleotide
Verfahren B,
deutsche Trink-
wasserverord-
nung Anlage 3,
No. 7
Water from water-
treatment plants
0.1 - 250.0 m-1 Absorption at 436 nm 302 100, 300, 600
525 nm DIN EN ISO
and protein components of
ribonucleoprotein complexes from cell extracts. Nature Protocols 1(1), 302-307 (2006).
2. Chen, Z. et al., RNase activity requires formation of disulfide bonds and is regulated
isolation system is
recommended to visualize the gel and avoid excess exposure to short wave UV
(302 nm or lower).
• If using the UV light, try to put the lane containing the ligated DNA
Immun 2004; 72: 3,706–3,715.
24. Lemarie SL, Horohov DW. Evaluation of interleukin-2 production
and interleukin-2 receptor expression in dogs with generalized
demodicosis. Vet Dermatol 1996; 7: 213–219
On the electrotransfer of polypeptides from gels
to nitrocellulose membranes Anal. Biochem. 128, 302-311 (1983)
2. Van Oss, C.J., et al., Mechanism of DNA (Southern) and protein (Western)
blotting