Brooklyn, Victoria 3025
AUSTRALIA
Phone +61 (0)3-9362-4500
Toll free-AUS 1 800-200-404
Fax +61 (0)3-9315-1656
Figure 1
2 to 8 C
Figure 2
2 to 8 C
15 to 30 C 37 C
37 C15 to
autoclavable storage
vessels and loosely attach the caps.
7. Sterilize the prepared medium in a validated autoclave at
121 C, 15 psi (1 kg/cm2) for 30 minutes on the liquid
cycle, slow exhaust. Because of
to final
volume.
6. Continue mixing for at least 30 minutes. To avoid fluctuation
in pH, keep the vessel closed until the medium is autoclaved.
7. Dispense the prepared medium into autoclavable storage
pressure at
3 - 15 psi. Do not use CO2 gas.
7. Sterile solutions should be dispensed aseptically into sterile
containers. Store liquid salt solutions at 15 to 30 C.
United States
SAFC Biosciences, Inc
Brooklyn, Victoria 3025
AUSTRALIA
Phone +61 (0)3-9362-4500
Toll free-AUS 1 800-200-404
Fax +61 (0)3-9315-1656
E-mail info-ap@sial.com
www.safcbiosciences.com
medium is filtered.
7. To sterilize the
bring it to
final volume. Continue mixing for at least 30 minutes. To
avoid fluctuation in pH, keep the vessel closed until the
medium is filtered.
7. To sterilize the medium, sterile filter using a low
in your own laboratory.
6. After autoclaving, allow media to cool to 15 to 30 C in
the dark, or protect from light.
7. For each 100 mL bottle being prepared, add 2.93 mL/L
of sodium bicarbonate solution