Manabu Yoshida et al.
Legal medicine (Tokyo, Japan), 11 Suppl 1, S357-S359 (2009-04-04)
Quantitative high performance liquid chromatography (HPLC) to detect semen was investigated in this study. Briefly, 1cm of a gauze thread with a seminal stain was soaked in the reaction mixture (phenolphthalein diphosphate tetrasodium dissolved in acetate buffer) for 5-10 min