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Shen Han et al.
Se pu = Chinese journal of chromatography, 29(7), 613-617 (2011-11-22)
A method for the determination of five aflatoxins (B1 , B2, G1 , G2, M1 ) in Chinese patent medicines and medicinal herbs by immunoaffinity extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was developed. The samples were
Xiaomeng Wu et al.
The Analyst, 137(18), 4226-4234 (2012-07-27)
High-quality surface-enhanced Raman scattering (SERS) spectra of aflatoxin (AF) B(1), B(2), G(1) and G(2) have been acquired using silver nanorod (AgNR) array substrates fabricated by oblique angle deposition method. Significant vibrational peaks are identified on the argon plasma-cleaned substrates, and
Chunping Liu et al.
Toxicology letters, 232(3), 547-556 (2014-12-03)
Our previous studies showed oral gavage of aflatoxin G₁ (AFG₁) induced lung adenocarcinoma in NIH mice. We recently found that a single intratracheal administration of AFG₁ caused chronic inflammatory changes in rat alveolar septum. Here, we examine whether oral gavage
Gonzalo J Diaz et al.
Journal of AOAC International, 95(4), 1084-1088 (2012-09-14)
The stability of aflatoxins B1, B2, G1, and G2 was studied in solutions containing different concentrations of water, acetonitrile, and/or methanol, and in autosampler vials treated with nitric acid or silanized. When stored at room temperature (20 degrees C) for
Si Chen et al.
Analytical and bioanalytical chemistry, 405(5), 1623-1630 (2013-01-15)
This article describes the use of microwave-assisted extraction (MAE) as a pretreatment technique for the determination of aflatoxins B(1), G(1), B(2), and G(2) in grains and grain products. The optimal operation parameters, including extraction solvent, temperature, and time, were identified
Lei Bao et al.
Journal of AOAC International, 96(5), 1017-1018 (2013-11-29)
A collaborative study of a method for determination of aflatoxins (AFs) B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and LC with fluorescence detection, previously published in J. AOAC
Danila Moscone et al.
Methods in molecular biology (Clifton, N.J.), 739, 217-235 (2011-05-14)
A novel method for aflatoxin B (AFB) determination is proposed. The AFB determination is based on acetylcholinesterase (AChE, from electric eel) inhibition, and the AChE residual activity is determined using the colorimetric method (Ellman's method). To select and optimize the
Debopam Acharya et al.
Analytica chimica acta, 630(1), 82-90 (2008-12-11)
Broad-specific immunoassays are an emerging technology that allows simultaneous determination of a class of closely related small molecular weight analytes. The current approaches are mainly based on the competitive format and involve complicated or expensive processes hindering their wide application.
Lokman Alpsoy et al.
Human & experimental toxicology, 30(6), 515-519 (2010-07-16)
Aflatoxins have been shown to be hepatotoxic, carcinogenic, mutagenic and teratogenic to different species of animals. Besides, at low concentrations, Selenium (Se(4+)) is antimutagenic and anticarcinogenic while it is toxic, mutagenic and carcinogenic at high concentrations. In this study, we
Zengning Li et al.
Toxicology letters, 195(2-3), 169-173 (2010-04-13)
Aflatoxins (AF) are the mycotoxin with the carcinogenic effect produced by the fungi like Aspergillus flavus, including AFB(1), B(2), G(1), G(2), M(1), and so on. The detection rate of Aflatoxin G(1) (AFG(1)) is very high in the diet of residents
Zheng Han et al.
Analytica chimica acta, 664(2), 165-171 (2010-04-07)
An ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of aflatoxins B1, B2, G1, G2, M1 and M2 in traditional Chinese medicines (TCMs) was developed. The approach was characterized in details and a special focus was placed on
Haitao Shen et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 50(9), 3222-3228 (2012-06-20)
Our previous studies showed intragastric administration of Aflatoxin G(1) (AFG(1)) could induce lung adenocarcinoma, which derived from alveolar type II cells (AT-II cells). AT-II cells contribute to Aflatoxin B(1) (AFB(1)) metabolism and also serve as the potential progenitor cells for
M I Almeida et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 29(4), 694-703 (2012-02-10)
A total of 230 samples of processed rice and its sub-products or derived products were analysed to establish the co-occurrence of several mycotoxins. Samples were analysed in the period 2007-2009 due to the outbreak of beriberi associated with the consumption
Jinyang Zhang et al.
Hybridoma (2005), 28(1), 67-70 (2009-02-26)
Using semisolid methylcellulose HAT medium selection and non-competitive and competitive indirect enzyme-linked immunosorbent assay, three hybridomas that secreted antibodies for aflatoxin G(1) were selected after fusion of mouse SP2/0 myeloma cells with spleen cells isolated from BALB/c mice that had
Lei Bao et al.
Journal of AOAC International, 93(3), 936-942 (2010-07-16)
Edible oils are consumed directly, and used as ingredients in food, soaps, and skin products. However, oils such as olive oil, peanut oil, and sesame oil could be contaminated with aflatoxins, which are detrimental to human and animal health. A
L H Zhao et al.
Journal of applied microbiology, 110(1), 147-155 (2010-11-03)
To prepare, purify and characterize an extracellular enzyme from Myxococcus fulvus ANSM068, designated as myxobacteria aflatoxin degradation enzyme (MADE), which possesses degradation activity against aflatoxin B(1) (AFB(1) ), G(1) (AFG(1) ) and M(1) (AFM(1) ) in solution. The culture supernatant
Sergio José López Grío et al.
Journal of separation science, 33(4-5), 502-508 (2010-02-04)
A rapid and simple method was developed to determine aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed and pet foods by UHPLC-MS/MS. Because the complexity of the evaluated matrices, the proposed method is based on sonication extraction
Ahmed M Abdel-Hadi et al.
Toxins, 3(6), 647-659 (2011-11-10)
Aspergillus flavus and Aspergillus parasiticus are important pathogens of cotton, corn, peanuts and other oil-seed crops, producing toxins both in the field and during storage. We have designed three siRNA sequences (Nor-Ia, Nor-Ib, Nor-Ic) to target the mRNA sequence of
Marilena Muscarella et al.
Methods in molecular biology (Clifton, N.J.), 739, 203-210 (2011-05-14)
A high-performance liquid chromatographic method with on-line post-column photochemical derivatization and fluorimetric detection for the simultaneous separation and quantitative determination of aflatoxin (AF) B(1), B(2), G(1), and G(2) in foodstuffs and feed materials is reported.The chromatographic separation is accomplished by
Nicoleta Manuela Miliţă et al.
Bacteriologia, virusologia, parazitologia, epidemiologia (Bucharest, Romania : 1990), 55(1), 19-24 (2010-11-03)
Aflatoxins are secondary metabolites produced by a group of strains, mainly Aspergillus and Penicillium species. These mycotoxins are bifurano-coumarin derivatives group with four major products B1, B2, G1 and G2 according to blue or green fluorescence emitted in ultraviolet light
Sahib Alam et al.
Journal of food science, 75(2), M61-M64 (2010-05-25)
The efficacy of calcium propionate at 2 different doses (0.5% and 1%) against growth and aflatoxins production by Aspergillus flavus (A-2092) was investigated in vitro on Czapek yeast extract agar at different levels of water activity (a(w)) in the range
Massimiliano Cuccioloni et al.
Analytical chemistry, 80(23), 9250-9256 (2009-06-25)
Aflatoxins are extremely toxic metabolites from Aspergillus species that can adulterate a wide range of human foodstuff. Herein, we propose a novel assay designed as an analytical test for aflatoxin B1 and G1 (AFB1 and AFG1, respectively) that could represent
Magda M Aly et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 48(11), 3031-3034 (2010-05-18)
Different local and exported white cheese samples were collected from different markets in Jeddah during September 2008. Trace and heavy metals including Pb, Zn, Mn, Cu, Fe and Cd were analyzed using atomic absorption spectrometry. The concentration of the tested
Zengning Li et al.
Wei sheng yan jiu = Journal of hygiene research, 40(1), 50-52 (2011-03-26)
To explore the effect of AFG1 on the expression of human leukocyte antigen (HLA-I) molecule in human esophageal epithelial cells. Western Blot and RT-PCT analysis was used to explore the effect of AFG1 on the antigen presenting function of primary
C Lorini et al.
Annali di igiene : medicina preventiva e di comunita, 20(6), 553-562 (2009-02-26)
Aim of this survey is to identify those filamentous fungi which parasite Boletus edulis and its group and check the potential presence of secondary metabolites, specifically aflatoxin B1, total aflatoxins and ochratoxin A, in order to assess the risk to
Lina Liu et al.
Phytochemical analysis : PCA, 23(5), 469-476 (2012-02-11)
We report a rapid and accurate method to determine the natural occurrence of aflatoxins (AFs) B1, B2, G1 and G2 in medicinal herbs and the analysis of 174 commercial samples by this method. The aim of the present work is
Hongmei Zeng et al.
Applied microbiology and biotechnology, 90(2), 635-650 (2010-12-15)
In aflatoxin biosynthesis, aflatoxins G(1) (AFG(1)) and B(1) (AFB(1)) are independently produced from a common precursor, O-methylsterigmatocystin (OMST). Recently, 11-hydroxy-O-methylsterigmatocystin (HOMST) was suggested to be a later precursor involved in the conversion of OMST to AFB(1), and conversion of HOMST
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