PTPN12 (1-355), active (SRP5073) - Datasheet
PTPN12 (1-355), active, GST tagged, human
recombinant, expressed in E. coli cells
Catalog Number SRP5073
Storage Temperature –70 °C
Synonyms:
Trinchieri, G., et al., Progress in Growth Factor
Research, 4, 355, (1992).
2. Merberg, D., et al., Immunol. Today, 13, 77 (1992).
3. Gearing, D., et al., Cell, 66, 9 (1991).
4. Schoenhaut, D.
cells with tricine buffer.
3. Incubate cells with a mixture (0.5 mL) of BioTracker NTP-
Transporter Molecule (20 µM) and Cy3-dUTP (20 µM) in the tricine
buffer for 3 min (37 °C; 5% CO2).
4. Remove staining
carcinogenesis.
Nutrition and Cancer, 57, 28-37 (2007).
2. Dreyer, D.L., and Bertelli, D.J., The structure of
Zapotin. Tetrahedron, 23, 4607-4612 (1967).
3. Cuendet, M., et al., Zapotin prevents mouse
°C or after 3 days at 37 °C.
References
1. Lambert, N., and Freedman, R.B., Biochem. J., 213,
225-234 (1983).
2. Carmichael, D.F. et al., J. Biol. Chem., 252, 7163-
7167 (1977).
3. Land, S. et
medium for isolation of dermatophytes from clinical materials. - Arch.
Dermat. Syphil., 67; 355-361
3. Georg L.K., Ajello D. and. Papageorge C., 1953, Use of cycloheximide in the selective isolation
to mix, and incubate in
the dark at 37 C for 1 hour.
7. Read samples in a fluorescence plate reader
(ex = 335–355 nm/ and em = 495–510 nm).
Background readings obtained
Biol. Animal. 35(4), 178-182
(1999).
4. Bjare, U., Serum-free culture. Pharmacol. Ther.,
53(3), 355-374 (1992).
Precautions and Disclaimer MSDS is available upon
request or at sigma-aldrich.
, in Diagnostic Cytogenetics (R.-D. Wegner,
ed.). Springer-Verlag (Berlin, Heidelberg), pp. 335-
355 (1999).
Trizma is a registered trademark of Sigma-Aldrich Co.,
LLC.
GCY,RXR,MAM 12/18-
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker
cap);
incubate for 8 min at 37 °C in a thermoshaker
2. Add 50 µL of Lysis Buffer C (red cap) and incubate for 8 min at 37°C and 1,400 rpm in the
thermoshaker