excitation, 455 nm emission,
5 nm slit width. Zero the instrument using 2 ml stop
solution.
Procedure
The reaction is performed in lysis/assay buffer
containing 0.85 mM 4-MU-Gal at 37 °C. The reaction
Integrin α2 (1-10 µg/mL) for 30 min at 2-8 °C.
6. Plate cells and incubate 30 min. at 37 °C.
7. Wash plates 3 X with media, gently. Count
unattached cells.
8. Add 1 mL dissociation fluid and
determined according to
calibration procedure B. Keep the tubes on ice.
7. Cap tubes and incubate at 37°C.
8. Transfer 100 :l aliquots at 10 minute intervals
to vials containing 2 ml cold stop solution.
α2 for 30 min. at 4°C.
6. Plate cells and incubate 30 min. at 37°C.
7. Wash plates 3 X with media, gently. Count
unattached cells.
8. Add 1 ml dissociation fluid, count attached cells.
fish electric organs (torpedo and electric eel);
membrane complexes. Eur. J. Biochem., 11(3),
441-455 (1969).
2. Gilson, M.K. et al., Open “back door” in a molecular
dynamics simulation of acetylcholinesterase
the reverse side of the invoice or packing slip.
6. Kwiatkowski, A.V., et al., Neuron, 56, 441-455
(2007).
7. Furman, C., et al., J. Cell Biol., 179, 761-775
(2007).
ER,RC,PHC 12/12-1
Biochem., 35, 2437 (1996).
13. Shaw, E., Cold Spring Harbor Conferences on Cell Proliferation, 2, 455 (1975).
14. Powers, J.C. and Harper, J.W., "Inhibitors of Serine Proteases" in Proteinase Inhibitors
12.
5. Keller G. (2005). Genes Dev 19: 1129-1155.
6. Schatten G. et al. (2005). Nature Methods 2:455-463.
7. Trounson A. (2006). Endocrine Reviews 27:208-219.
For research
Parteck® LM low 63:1 20C
No 30.000 29.963 0.090 0.301
No 50.000 49.876 0.414 0.830
Mg-Stearate 455:1 10C
No 0.500 0.502 0.012 2.482
Yes 0.500 0.498 0.009 1.906
M
as
s
flo
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[
kg
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