Ankenbauer, W. & Schmitz, G. (1989) unpublished
results
2 Kessler, C. & Manta, V. (1989) Gene 74 (1): 179-181.
3 Blanck et al (1995) Biochemica 2: 14
4 Rebase The Restriction Enzyme Database:
http://
analysis.
References
1 Sato, S. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 542.
2 Kessler, C. & Manta, V. (1990) Gene 92,1-250.
3 Malyguine, E. et al. (1980) Gene 8, 163.
4 Kessler, C. & Manta, V. (1990
Conjugate, 1,000 U 11 089 161 001
Streptavidin-β-Gal Conjugate, 500 U, Not available in US 11 112 481 001
Streptavidin-POD Conjugate, 500 U 11 089 153 001
Protein Agarose Protein G Agarose, 2 ml 11
Rb 100 µL AB3844
Anti-PAK1/2/3 H M Mk R WB APur Rb 100 µL AB3845
PAK-PBD GST Protein, Rac/Cdc42 binding domain Pur SGT224
Protein
PAK2, active KA 10 µg 14-481
PAK3, active KA 10
475 Myosin M1570 4619 MYH1 M NP_005954.3 y y y
477 Myosin IIA M8064 4627 MYH9 P NP_002464.1 y n/d y
481 Myosin Light Chain Kinase M7905 4638 MYLK M NP_444253.3 n/d n/d n/d
476 Myosin Iβ (Nuclear) M3567 4641
475 Myosin M1570 4619 MYH1 M NP_005954.3 y y y
477 Myosin IIA M8064 4627 MYH9 P NP_002464.1 y n/d y
481 Myosin Light Chain Kinase M7905 4638 MYLK M NP_444253.3 n/d n/d n/d
476 Myosin Iβ (Nuclear) M3567 4641
475 Myosin M1570 4619 MYH1 M NP_005954.3 y y y
477 Myosin IIA M8064 4627 MYH9 P NP_002464.1 y n/d y
481 Myosin Light Chain Kinase M7905 4638 MYLK M NP_444253.3 n/d n/d n/d
476 Myosin Iβ (Nuclear) M3567 4641
Rb 100 µL AB3844
Anti-PAK1/2/3 H M Mk R WB APur Rb 100 µL AB3845
PAK-PBD GST Protein, Rac/Cdc42 binding domain Pur SGT224
Protein
PAK2, active KA 10 µg 14-481
PAK3, active KA 10
d y
478 Myosin IX/Myr5 M5566 P n/d n/d y
479 Negative Control NA
480 Anti Cy3+Cy5 C0992 M NA NA NA
481 Myosin Light Chain Kinase M7905 M y y Y
482 Myosin Va M4812 P n/d n/d y
483 Myosin Va M5062 P n/d
478 Myosin IX/Myr5 M5566 25486 Myo9b NP_037116.1 P n/d n/d y
479 Negative Control
480 Anti Cy3+Cy5
481 Myosin Light Chain Kinase M7905 4638 MYLK NP_444260.1 M y y Y
482 Myosin Va M4812 25017, 17918, 4644
Myosin IX/Myr5 M5566 25486 Myo9b NP_037116.1 P n/d n/d y
479 Negative Control NA
480 Anti Cy3+Cy5 C0992
481 Myosin Light Chain Kinase M7905 4638 MYLK NP_444260.1 M y y Y
482 Myosin Va M4812 25017, 17918, 4644