Percentage of Improvement
Decreased solvent usage by 76%
Reduced quantity of chemical usage
tBuXPhos (638080)
N/A
N/A
N/A
N/A
0= Most Desirable
92%
Reduced chemical processing
adsorption and subse-
quent elution.
References
1. Broze, G. J., Jr., et al., J. Clin. Invest., 76, 937
(1985).
2. Broze, G. J., Jr., and Majerus, P. W., J. Biol. Chem.,
255, 1242 (1980).
al., Methods in Cell Biology, 30,
449 (1989).
5. Wulf, E. et al., Proc. Nat. Acad. of Sci., USA, 76,
4498 (1979).
6. Faulstich, H. et al., Exp. Cell Res., 144, 73 (1983).
7. Lawson, M.A., and Maxfield
Amicon® Stirred Cells.
Amicon® Stirred Cells
Volume Membrane Diameter
50 mL 44.5 mm
200 mL 63.5 mm
400 mL 76 mm
http://www.merckmillipore.com?cid=BI-XX-BSP-C-EPDF-ASP0-B005-1601
Netherlands
t 31 20 655 79 28
f 31 20 659 76 73
United Kingdom
t 0800 376 8660
f 0800 279 1117
All Other European
Countries
t 31 (0) 20 659 60 51
f 31 (0) 20 659 76 73
L AT I N AME
to spin to 50 µL retentate at 3000 x g, 25 °C
Initial Volume of Time to Spin to
0.5 mg/mL 50 µL Retentate
Cytochrome c (µL) (minutes)
100 µL 15
200 µL 25
requirement
Specific rotation
10 mg per mL in dioxane 〈781 S〉
between +72° and +80° +76°
Loss on drying 〈731〉 not more than 0.5% meets requirement
Residue on
meets requirement
Specific rotation
10 mg per mL in dioxane 〈781 S〉
between +72° and +80° +76°
Loss on drying 〈731〉 not more than 0.5% meets requirement
Residue on ignition 〈281〉 not more
N-dimethylformamide
(DMF, 10 mg/ml) and pyridine:1 M HCl (1:1, 50 mg/ml)
yielding a clear, colorless solution. It has been used at
0.2 mM (76 µg/ml) in 0.2 M acetate buffer, pH 4.3).2
References
7.5, containing 150 mM sodium chloride,
100 µg/ml bovine serum albumin and 50% glycerol.
Storage/Stability
Store at 0 °C to −20 °C. If slight turbidity occurs upon
prolonged storage, clarify the
Process Volume: 0.075 mL 1.0 mL 2.5 mL 5.0 mL 10.0 mL
Nominal Membrane Diameter: 25 mm 25 mm 43 mm 62 mm 76 mm
Effective Membrane Area: 0.9 cm 4.1 cm 13.4 cm 28.7 cm 41.8 cm
Hold-up Volume:* 0.07 mL 0.2