of starter culture to 50 mL of
fresh LB/Carb/Chl medium and grow at 37 C.
5. Harvest cells at OD600 0.9 by centrifugation.
6. Resuspend harvested cells (step 4) in 50 mL of
sterile
of starter culture to 50 mL of
fresh LB/Carb/Chl medium and grow at 37 C.
5. Harvest cells at OD600 0.9 by centrifugation.
6. Resuspend harvested cells (step 4) in 50 mL of
sterile
of starter culture to 50 mL of
fresh LB/Carb/Chl medium and grow at 37 C.
5. Harvest cells at OD600 0.9 by centrifugation.
6. Resuspend harvested cells (step 4) in 50 mL of
sterile
71,
245-50 (2006).
8. Couderc, R., and Baratti, J., Oxidation of methanol
by the yeast Pichia pastoris: purification and
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44, 2279-89 (1980).
(w/v)
Bromphenol Blue, 0.1% (w/v) Xylene Cyanole FF,
and 20 mM EDTA.
5. TBE Running Buffer: 89 mM Tris base, 2 mM
EDTA, and 89 mM boric acid, pH 8.0.
6. Putrescine Solution: 0.5 M putrescine free
characterization of a mouse homolog of human
TNFSF14, a member of the TNF superfamily.
Cytogenet., 89, 89 – 91, (2000)
2. Montgomery, R., et al., Herpes simplex virus-1 entry
into cells mediated by
Operating temperature 1°C to 40°C
Safety compliance 115 V CSA approved
220 V CE compliant - 89/356/EEC
89/392/EEC
91/368/EEC
Ordering Information
Description Catalogue Number
Vacuum/pressure pump
Rinse with gently running tap water for 5 minutes.
4. Transfer to distilled water .
5. Proceed to STAINING PROCEDURE.
3Sigma Technical Bulletin SE-1 (1-89)
STAINING PROCEDURE
1. Rinse
Rinse with gently running tap water for 5 minutes.
4. Transfer to distilled water .
5. Proceed to STAINING PROCEDURE.
3Sigma Technical Bulletin SE-1 (1-89)
STAINING PROCEDURE
1. Rinse
Rinse with gently running tap water for 5 minutes.
4. Transfer to distilled water .
5. Proceed to STAINING PROCEDURE.
3Sigma Technical Bulletin SE-1 (1-89)
STAINING PROCEDURE
1. Rinse
substrate 2 µl (~5 pmole)
Distilled water 68 µl
3. Dilute the endo III enzyme to 25, 50, and
100 µg/ml with enzyme dilution buffer.
4. Dispense 8 µl of reaction mix into each tube
5.
Goat anti-Mouse IgG, Cat. No.: AP124, dilution 1:50-100) for 1-2 hours.
5. Incubate with diluted PAP complex (i.e. Mouse PAP, Cat No.: PAP14, conc. 25-50 µg/mL) for one hour.
6. After rinsing in buffer
attaching the tube to a sampling pump.
25097-U TDS3 Storage Container for 89 mm tubes
25073 Replacement Septa, pk. 50
25069 Sample Cap Set w/washers for TDS3
Empty Tubes
Empty tubes are available
Di Paolo, G. et al., Nature, 420, 85-89 (2002).
3. Ling, K. et al., Nature, 420, 89-93 (2002).
4. Morgan, J.R. et al., J. Cell Biol., 167, 43-50 (2004).
5. Wenk, M.R. et al.
Di Paolo, G. et al., Nature, 420, 85-89 (2002).
3. Ling, K. et al., Nature, 420, 89-93 (2002).
4. Morgan, J.R. et al., J. Cell Biol., 167, 43-50 (2004).
5. Wenk, M.R. et al.
non-neuroendocrine
tumors. About 50% of neuroendocrine tumors have
elevated concentration of CgA. Serum CgA is most
frequently elevated in gastrinoma (100%),
pheochromocytoma (89%), carcinoid tumors (80%)