50200

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Dipeptide forms of glycine support mouse preimplantation embryo development in vitro and provide protection against high media osmolality.

Molly Moravek et al.

Journal of assisted reproduction and genetics, 29(3), 283-290 (2012-01-17)

To examine potential benefits of dipeptide forms of amino acids for embryo culture by determining ability of dipeptide glycine forms to support embryo development, act as osmolytes, and reduce ammonia production. Frozen thawed 1-cell mouse embryos were cultured in media

Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.

Cheng-Han Tsai et al.

Biochimica et biophysica acta, 1852(5), 851-861 (2015-01-20)

Cofilin-1, a non-muscle isoform of actin regulatory protein that belongs to the actin-depolymerizing factor (ADF)/cofilin family is known to affect cancer development. Previously, we found that over-expression of cofilin-1 suppressed the growth and invasion of human non-small cell lung cancer

Arabidopsis HFR1 is a potential nuclear substrate regulated by the Xanthomonas type III effector XopD(Xcc8004).

Choon Meng Tan et al.

PloS one, 10(2), e0117067-e0117067 (2015-02-04)

XopDXcc8004, a type III effector of Xanthomonas campestris pv. campestris (Xcc) 8004, is considered a shorter version of the XopD, which lacks the N-terminal domain. To understand the functions of XopDXcc8004, in planta, a transgenic approach combined with inducible promoter

Neochromine S5 improves contact hypersensitivity through a selective effect on activated T lymphocytes.

Zhe Gao et al.

Biochemical pharmacology, 92(2), 358-368 (2014-09-15)

Strategy on activated T cells is an effective treatment for T cell mediated diseases. By using a synthesized chromone derivative, we examined its effects on the activated T cells. This compound, (Z)-1,3-dihydroxy-9-methyl-13H-benzo[b]chromeno[3,2-f][1,4]oxazepin-13-one (neochromine S5), exhibited immunosuppressive activity in vitro and

Characterizing ubiquitination sites by peptide-based immunoaffinity enrichment.

Daisy Bustos et al.

Molecular & cellular proteomics : MCP, 11(12), 1529-1540 (2012-06-26)

Advances in high resolution tandem mass spectrometry and peptide enrichment technologies have transformed the field of protein biochemistry by enabling analysis of end points that have traditionally been inaccessible to molecular and biochemical techniques. One field benefitting from this research

Polyglutamine toxicity in yeast induces metabolic alterations and mitochondrial defects.

Katharina Papsdorf et al.

BMC genomics, 16, 662-662 (2015-09-04)

Protein aggregation and its pathological effects are the major cause of several neurodegenerative diseases. In Huntington's disease an elongated stretch of polyglutamines within the protein Huntingtin leads to increased aggregation propensity. This induces cellular defects, culminating in neuronal loss, but

Pharmacokinetic and pharmacodynamic evaluation of tigecycline.

Helen Giamarellou et al.

Expert opinion on drug metabolism & toxicology, 7(11), 1459-1470 (2011-10-01)

As the spread of multidrug-resistant (MDR) and extensive drug-resistant (XDR) organisms constitutes a real threat for patients, new antimicrobials are needed. Tigecycline, the first-in-class glycylcycline, possesses an extended spectrum of antimicrobial activity including MDR and XDR organisms, which holds promise

Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy.

Doori Oh et al.

Chemical communications (Cambridge, England), 47(32), 9137-9139 (2011-07-14)

In a free solution of 10.0 mM Gly-Gly (pH 8.2), the flow directions of native-DNA and DNA molecules intercalated with the fluorescent dye YOYO-1 were reversed in the microchip channel. These results clearly showed that in a confined space and

GlyGly-CTERM and rhombosortase: a C-terminal protein processing signal in a many-to-one pairing with a rhomboid family intramembrane serine protease.

Daniel H Haft et al.

PloS one, 6(12), e28886-e28886 (2011-12-24)

The rhomboid family of serine proteases occurs in all domains of life. Its members contain at least six hydrophobic membrane-spanning helices, with an active site serine located deep within the hydrophobic interior of the plasma membrane. The model member GlpG

Inhibition of calcium-calmodulin complex formation by vasorelaxant basic dipeptides demonstrated by in vitro and in silico analyses.

Thanutchaporn Kumrungsee et al.

Biochimica et biophysica acta, 1840(10), 3073-3078 (2014-08-06)

Tryptophan-histidine (Trp-His) was found to suppress the activity of the Ca²⁺/calmodulin (CaM)-dependent protein kinases II (CaMKII), which requires the Ca²⁺-CaM complex for an initial activation. In this study, we attempted to clarify whether Trp-His inhibits Ca²⁺-CaM complex formation, a CaMKII

Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.

Sebastian A Wagner et al.

Molecular & cellular proteomics : MCP, 11(12), 1578-1585 (2012-07-14)

Posttranslational modifications of proteins increase the complexity of the cellular proteome and enable rapid regulation of protein functions in response to environmental changes. Protein ubiquitylation is a central regulatory posttranslational modification that controls numerous biological processes including proteasomal degradation of

Impairment of jejunal absorption rate of carnosine by glycylglycine in man in vivo.

G C Cook

Gut, 17(4), 252-257 (1976-04-01)

Using a double-lumen tube jejunal perfusion system in vivo, the mutual effects of carnosine (beta-alanyl-L-histidine) and glycylglycine on their respective absorption rates have been studied in six Zambian African adults. Data on the effect of the constituent amino-acids of carnosine

Glycine and diglycine as possible catalytic factors in the prebiotic evolution of peptides.

Plankensteiner K

Origins of Life and Evolution of the Biosphere : the Journal of the International Society For the Study of the Origin of Life, 32(3), 225-236 (2002)

Thermodynamics and mechanisms of protonated diglycine decomposition: a guided ion beam study.

P B Armentrout et al.

Journal of the American Society for Mass Spectrometry, 23(4), 632-643 (2011-09-29)

We present a full molecular description of fragmentation reactions of protonated diglycine (H(+)GG) by studying their collision-induced dissociation (CID) with Xe using a guided ion beam tandem mass spectrometer (GIBMS). Analysis of the kinetic energy-dependent CID cross sections provides the

Characterization of the C-terminal diglycine motif of SUMO-1/3.

Koji Yamada et al.

Bioscience, biotechnology, and biochemistry, 76(5), 1035-1037 (2012-06-29)

A hallmark of small ubiquitin-related modifier (SUMO) is the production of a C-terminal tail containing diglycines (GGs), which are believed to be required for SUMOylation. Whether GGs are required components in SUMOylation remains unanswered experimentally. In this study we found

Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.

Namrata D Udeshi et al.

Molecular & cellular proteomics : MCP, 12(3), 825-831 (2012-12-26)

Detection of endogenous ubiquitination sites by mass spectrometry has dramatically improved with the commercialization of anti-di-glycine remnant (K-ε-GG) antibodies. Here, we describe a number of improvements to the K-ε-GG enrichment workflow, including optimized antibody and peptide input requirements, antibody cross-linking

Recurrence of carbamoyl phosphate synthetase 1 (CPS1) deficiency in Turkish patients: characterization of a founder mutation by use of recombinant CPS1 from insect cells expression.

Liyan Hu et al.

Molecular genetics and metabolism, 113(4), 267-273 (2014-11-21)

Carbamoyl phosphate synthetase 1 (CPS1) deficiency due to CPS1 mutations is a rare autosomal-recessive urea cycle disorder causing hyperammonemia that can lead to death or severe neurological impairment. CPS1 catalyzes carbamoyl phosphate formation from ammonia, bicarbonate and two molecules of

Thermodynamics and mechanisms of protonated diglycine decomposition: a computational study.

P B Armentrout et al.

Journal of the American Society for Mass Spectrometry, 23(4), 621-631 (2011-09-29)

We present a full computational description of the fragmentation reactions of protonated diglycine (H(+)GG). Relaxed potential energy surface scans performed at B3LYP/6-31 G(d) or B3LYP/6-311 + G(d,p) levels are used to map the reaction coordinate surfaces and identify the transition states (TSs) and

Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.

Francis Impens et al.

Proceedings of the National Academy of Sciences of the United States of America, 111(34), 12432-12437 (2014-08-13)

SUMOylation is an essential ubiquitin-like modification involved in important biological processes in eukaryotic cells. Identification of small ubiquitin-related modifier (SUMO)-conjugated residues in proteins is critical for understanding the role of SUMOylation but remains experimentally challenging. We have set up a

Identification of a neutralizing epitope within antigenic domain 5 of glycoprotein B of human cytomegalovirus.

Anna-Katharina Wiegers et al.

Journal of virology, 89(1), 361-372 (2014-10-17)

Human cytomegalovirus (HCMV) is an important, ubiquitous pathogen that causes severe clinical disease in immunocompromised individuals, such as organ transplant recipients and infants infected in utero. The envelope glycoprotein B (gB) of HCMV is a major antigen for the induction

Validation of the 2nd Generation Proteasome Inhibitor Oprozomib for Local Therapy of Pulmonary Fibrosis.

Nora Semren et al.

PloS one, 10(9), e0136188-e0136188 (2015-09-05)

Proteasome inhibition has been shown to prevent development of fibrosis in several organs including the lung. However, effects of proteasome inhibitors on lung fibrosis are controversial and cytotoxic side effects of the overall inhibition of proteasomal protein degradation cannot be

Metabolite Profiling Identifies a Key Role for Glycine in Rapid Cancer Cell Proliferation

Mohit Jain

Science, 336(6084), 1040-1044 (2012)

Contribution of autoallergy to the pathogenesis in the NOD mice.

Radha Thyagarajan et al.

Autoimmunity, 48(5), 298-304 (2015-02-25)

The immunoglobulin isotype IgE is commonly associated with allergy. However, its involvement in autoimmune disease in general, and Type 1 diabetes (T1D) in particular, is still not completely clarified, nonetheless IgE has been observed in patients with T1D. In this

Abiogenic Syntheses of Lipoamino Acids and Lipopeptides and their Prebiotic Significance.

Gordon Sproul

Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life, 45(4), 427-437 (2015-08-08)

Researchers have formed peptide bonds under a variety of presumed prebiotic conditions. Here it is proposed that these same conditions would have also formed amide bonds between fatty acids and amino acids, producing phosphate-free amphipathic lipoamino acids and lipopeptides. These

Angiotensin-converting enzyme for noninvasive assessment of liver fibrosis in autoimmune hepatitis.

Cumali Efe et al.

European journal of gastroenterology & hepatology, 27(6), 649-654 (2015-04-11)

There are no validated noninvasive markers of liver fibrosis in autoimmune hepatitis (AIH). An activated renin-angiotensin system (RAS) and its key element angiotensin-converting enzyme (ACE) have been implicated in the pathogenesis of hepatic fibrogenesis. We aimed to study the assumed

Determination of the isoflavone composition and estrogenic activity of commercial dietary supplements based on soy or red clover.

Susanne Andres et al.

Food & function, 6(6), 2017-2025 (2015-05-30)

Dietary supplements high in isolated isoflavones are commercially available for human consumption primarily to alleviate menopausal symptoms in women. The isoflavone composition, quantity and importantly their estrogenic potency are poorly standardised and can vary considerably between different products. The aim

Amino acids: metabolism, functions, and nutrition.

Wu G

Amino Acids, 37(1), 1-17 (2009)

Analogy to a Chinese knot in an ion-pair copper(II)-neodymium(III) complex based on a hexadentate Schiff base condensation product of 5-bromosalicylaldehyde and glycylglycine.

Li-Qing Xu et al.

Acta crystallographica. Section C, Crystal structure communications, 69(Pt 4), 376-379 (2013-04-13)

Self-assembly of CuCl2, NdCl3, 5-bromosalicylaldehyde and glycylglycine yields the ion-pair copper(II)-neodymium(III) complex, poly[[decaaquabis[μ3-2-({2-[(5-bromo-2-oxidobenzylidene)amino]acetyl}azanidyl)acetato]bis[μ2-2-({2-[(5-bromo-2-oxidobenzylidene)amino]acetyl}azanidyl)acetato]tetracopper(II)dineodymium(III)] bis{[2-({2-[(5-bromo-2-oxidobenzylidene)amino]acetyl}azanidyl)acetato]cuprate(II)} tetradecahydrate], {[Cu4Nd2(C11H8BrN2O4)4(H2O)10][Cu(C11H8BrN2O4)]2·14H2O}n. The anion is planar and mononuclear, showing an approximately square-planar coordination of the metal atom, while the cation is a hexanuclear centrosymmetric transition metal-lanthanide

Umami-bitter interactions: the suppression of bitterness by umami peptides via human bitter taste receptor.

Min Jung Kim et al.

Biochemical and biophysical research communications, 456(2), 586-590 (2014-12-10)

Taste-taste interactions often showed in human psychophysical studies. Considering that each tastant in foodstuffs individually stimulates its responsible gustatory systems to elicit relevant taste modalities, taste-taste interaction should be performed in taste receptor cell-based assay. While umami substances have been

Estimating statistical distributions using an integral identity.

Cheng Zhang et al.

The Journal of chemical physics, 136(20), 204113-204113 (2012-06-07)

We present an identity for an unbiased estimate of a general statistical distribution. The identity computes the distribution density from dividing a histogram sum over a local window by a correction factor from a mean-force integral, and the mean force

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