Fisher). Mobile phases were sonicated prior to use.
Instrumentation
The HPLC system was from Waters: 510 HPLC pumps, 717 plus autosampler, and a 996 Photodiode Array Detector set
at 190 – 400 nm. Columns
assessed
using the dye Neutral Red. The absorbance maximum
of the Neutral Red dye shifts from 460 nm to 510 nm
in the acidic pH conditions found in the lysosome. The
Neutral Red dye will concentrate in
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
Biochem. J., 219(2), 505-510 (1984).
18. Stein, B.S., and Sussman, H.H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
Biochem. J., 219(2), 505-510 (1984).
18. Stein, B.S., and Sussman, H.H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
Biochem. J., 219(2), 505-510 (1984).
18. Stein, B.S., and Sussman, H.H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
Biochem. J., 219(2), 505-510 (1984).
18. Stein, B.S., and Sussman, H.H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
Biochem. J., 219(2), 505-510 (1984).
18. Stein, B.S., and Sussman, H.H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
Biochem. J., 219(2), 505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide mapping
of the human transferrin receptor in normal and
transformed cells. J. Biol. Chem., 258(4), 2668-
2673 (1983).
performed with a Waters Micromass® ZQ® 2000
Drug mixture study:
• Instrumentation
HPLC system: Waters 510 HPLC pumps; 717 plus autosampler; 996 Photodiode Array Detector set at 190 – 400 nm
Columns: SymmetryShield
2000; 48(12): 661-672.
3. Cancer Res. 1990; 50(3): 492-498.
4. Sci Transl Med. 2017; 9(408): eaan4220
5. Clin Cancer Res. 2009; 15(2): 502-510.
FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES
. J., 219(2),
505-510 (1984).
18. Stein, B. S., and Sussman, H. H., Peptide
mapping of the human transferrin receptor in
normal and transformed cells. J. Biol. Chem.,
258(4), 2668-2673 (1983).
Pro Software
HPLC analyses were completed with a Waters HPLC-UV system equipped with two Waters 510 pumps
and Waters WAT46980 C-18 column with a guard cartridge using Waters Millennium 3.2 software.