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alpha-Synuclein ELISA Kit Instruction Manual
disruption of cells.
520 µµµµl
Working
Stock
520 µµµµl 520 µµµµl 520 µµµµl 520 µµµµl 520 µµµµl
92.3 60 39 25.4 16.5 10.7 7.0
8
4.5
Product Information Sheet - 330003S
recorded at 520 nm.
Fig. 3 Sample Direct ELISA
Immobilized antigens, Cu-Ox LDL and LDL probed with EO6-TopFluor™ antibody.
E06-TopFluor™ binding was measured as fluorescence emission at 520 nm.
EO6
Product Information Sheet - E7637
glycine buffer)4
Solvent effects on absorbance:
λmax = 480 nm (water); 515 (glycerol); 520 nm
(methanol): 520 nm (acetone); 532 nm (ethanol); 535
(DMSO) and 540 nm (pyridine).5 The absorbance
maximum
BioFiles-Issue
75000 Ar laser
Atto 488 501 523 80 90000 Ar laser FITC, Alexa 488
Atto 495 499 535 45 80000 Ar laser
Atto 520 525 545 90 110000 Ar laser JOE
Atto 532
Product Information Sheet - E1385
glycine buffer)4
Solvent effects on absorbance:
λmax = 480 nm (water); 515 (glycerol); 520 nm
(methanol): 520 nm (acetone); 532 nm (ethanol); 535
(DMSO) and 540 nm (pyridine).5 The absorbance
maximum
Quality Control Range Sheet- TH17-113 and TH17-213
Control 2 673 – 1397 pg/mL
IL-12P70 Control 1 51 – 106 pg/mL IL-6 Control 1 23 – 47 pg/mL
Control 2 520 – 1079
Product Information Sheet - MAK327
Pharmacology, Mechanism of Action, Substrate
Specificity, and Clinical Application. Pharmacol.
Rev., 64(3), 520-539 (2012).
HM,MAM 11/18-1
2018 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-
Product Information Sheet-dctp-ro
For general laboratory use.
y Version: 09
Content Version: July 2021
Cat. No. 11 934 520 001 250 μl
25 μmol, 100 mM
6,250 standard PCR assays of 20 μl each.
Cat. No.
STED Sample Preparation Guide
475–510 Oregon Green 488/
Chromeo 505
514/520 523–580
Oregon Green 488 470 (WLL) 480–520 Alexa Fluor 532 545 (WLL) 555–580
Alexa Fluor 532 514 520–565 TMR/TRITC/
Alexa Fluor 568
580 590–650
Product Information Sheet - A9264
K., Eur. J. Biochem., 53, 481-486 (1975).
5. Yamazaki, Y. et al., Eur. J. Biochem., 77, 511-520 (1977).
6. Haystead, C.M.M. et al., Eur. J. Biochem., 214, 459-467 (1993).
7. Bar-Tana, J. and Cleland
hTRPV1-HEK293 Recombinant Cell Line
each cell passage is shown in Figure 6.
Current amplitudes were typically consistently in excess of 2 nA and expression
levels above 60% for > 45 passages.
Figure 6. Stability of expression over passage
Product Information Sheet - - F1804
by fluorescence microscopy. FITC has an
absorption maximum at 492 nm with an emission
maximum at 520 nm.
Immunoprecipitation (IP)
Monoclonal ANTI-FLAG® M2 may be used in IP
procedures when used
XP725 Antibody Number-Lot Number 071M4826
.3 489 490 491 492 513 514 515 516 537 538 539 540 561 562 563 564
6.4 493 494 495 496 517 518 519 520 541 542 543 544 565 566 567 568
6.5 497 498 499 500 521 522 523
Product Information - FD4
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD70S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD500S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD40S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD150S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD2000S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD70
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD40
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD20S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information Sheet - FD250S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD20
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Product Information - FD10S
FLUORESCENT PROPERTIES:
The excitation maximum of FITC-dextran is 490 nm. The emission maximum is 520 nm.11 The
fluorescence increases with pH and is optimal at pH 8 and above.11
SYNONYM: FITC-Dextran
Protease Substrate Set
min to
2 hours (45 min recommended) at 37°C. Please note that reaction
conditions might need to be optimized for your protease regarding
incubation temperatures and reaction time.
6. Measure fluorescence
Data Sheet - G5527
applications dilutions may range from A =1.0-0.05 (1:5-1:100 dilution), Incubation times range from 0.5-520
12 hours.
DESCRIPTION:
Gold-antibody conjugates are used to detect antigens and require no additional
Caspase Substrate Set
sure that the final volume in
all wells is similar.
6. Incubate the microtiter plate with the protease solution for 20 min to
2 hours (45 min recommended) at 37°C. Please note that reaction
conditions
Anti-NeuN, clone A60 - Data Sheet
2004) 24:853-864.
50) Fricker-Gates, R.A., et al., European Journal of Neuroscience (2004) 19:513-520.
51) Jakobsson, J., et al., European Journal of Neuroscience (2004) 19:761-765.
52) Bloechlinger
Data Sheet - G5528
applications dilutions may range from A =1.0-0.05 (1:5-1:100 dilution), Incubation times range from 0.5-520
12 hours.
DESCRIPTION:
Gold-antibody conjugates are used to detect antigens and require no additional
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