incubation at 37°C. Note that the NovaBlue strain carries the relA1 mutation,
so is unsuitable for amplification by this method.
Cat. No. Page
USA and Canada
incubation at 37°C. Note that the NovaBlue strain carries the relA1 mutation,
so is unsuitable for amplification by this method.
Cat. No. Page
USA and Canada
Tel
volume
Add T4 Polynucleotide Kinase last. Mix gently by stirring with a pipet tip.
4. Incubate at 37°C for 10 min.
5. Add 20 µl annealing buffer (prepared in Step 1) and 65 µl sterile water
transfer vial to 37°C
water bath.
3. Thaw cells quickly by immersing vial about halfway into 37°C water bath. Gently swirl vial
until cells are fully thawed (approximately 2 min).
4. Sterilize exterior
About the Kits
USA and Canada
Tel (800) 526-7319
novatech@novagen.com
Germany
Tel 0800 100 3496
techservice@merckbiosciences.de
United Kingdom and Ireland
Media for isolation-cultivation-identification-maintenance of medical bacteria,
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6. R.R. Facklam, J.A. Washington II., Streptococcus and
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