recognized by CD45 monoclonal antibody
(BRA-55) is sensitive to formalin fixation and paraffin
embedding.
Performance
When assayed by flow cytometric analysis, using 10 µl
of the conjugate to stain 1 X 106
+65 Mae III +55
Acy I +50 Bsp LU 11 +48 Sfi I +50
Apa I +30 Bss HII +50 Sma I +25
Bcl I +50 Bst EII +60 Swa I +25
Bse AI +55 Bst XI +45 Taq I +65
Bsi WI +55 Mae
+65 Mae III +55
Acy I +50 Bsp LU 11 +48 Sfi I +50
Apa I +30 Bss HII +50 Sma I +25
Bcl I +50 Bst EII +60 Swa I +25
Bse AI +55 Bst XI +45 Taq I +65
Bsi WI +55 Mae
+65 Mae III +55
Acy I +50 Bsp LU 11 +48 Sfi I +50
Apa I +30 Bss HII +50 Sma I +25
Bcl I +50 Bst EII +60 Swa I +25
Bse AI +55 Bst XI +45 Taq I +65
Bsi WI +55 Mae
with
100 µL of wash buffer (7 mM Tris, pH 8.0, 0.17 M
NaCl) twice.
9. Elute the bound mRNA with 25-50 µL of DEPC
treated water at 55 °C for two minutes.
Escherichia coli
ATCC 8739 • WDCM 00012
/ 55’ at 20-25°C +/- 30% of the number of colonies counted at T0
Listeria monocytogenes
ATCC 13932 • WDCM 00021
/ 55’ at 20-25°C +/- 30%
synthetic resins.7
As a fixative in electron microscopy, it is necessary to use a methanol-free preparation.6
Paraformaldehyde is then added to basic buffer or water, heated with stirring to 55-60°C until
warm at 55–65 °C until the precipitate
dissolves and cool to room temperature before use.
4. Dilute Wash Solution
Concentrate
Dilute the Wash Solution Concentrate (B6553) with 10
mL (10 prep package
. Wood, J.N., et al., Voltage-gated sodium channels
and pain pathways, J. Neurobiol., 61, 55-71 (2004).
7. Renganathan, M. et al., Expression of Nav1.8
sodium channels perturbs the firing patterns
. Wood, J.N., et al., Voltage-gated sodium channels
and pain pathways, J. Neurobiol., 61, 55-71 (2004).
7. Renganathan, M. et al., Expression of Nav1.8
sodium channels perturbs the firing patterns
warm at 55–65 °C until the precipitate
dissolves and cool to room temperature before use.
4. Dilute Wash Solution
Concentrate
Dilute the Wash Solution Concentrate (B6553) with 10
mL (10 prep package