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Product Information Sheet-11465007001
tissue-culture grade, flat-bottom microplates. 100 24 – 96 +37 2 Add MTT labeling reagent and incubate in a humidified atmosphere. 10 4 +37 3 Add Solubilization buffer and incubate in a humidified
Product Information Sheet - CYTOCOX1
cytochrome c oxidase activity The absorption of cytochrome c at 550 nm changes with its oxidation state. This property is the basis for the assay.3 Cytochrome c is reduced with dithiothreitol and then reoxidized
Data Sheet - 44653
by the MPN technique, J. Food Prot. 55, 266 (1992) 2. Hajna and Perry, Am. 1 Publ. Health, 33:550 (1943) 3. Tennant et al., Can. J. Microbiol., 1:733 (1961) 4. Fishbein and Surkiewicz, Appl. Microbiol
(C4519) - Enzyme Assay
3.3 mM sodium chloride and 3.0 units ceruloplasmin. SPDIME03 Page 3 of 3 Revised: 06/24/05 REFERENCE: Curzon, G. and
(C2026) - Enzyme Assay
3.3 mM sodium chloride and 3.0 units ceruloplasmin. SPDIME03 Page 3 of 3 Revised: 06/24/05 REFERENCE: Curzon, G. and
Data Sheet - 71882
1. Hajna A.A., a. Perry C.A., 1943, Am. J. Pbl. Hlth. 33:550-556. 2. Perry, C. A. and Hajna, A. A. (1944) Amer. J. Pub. Hlth. 34, 735-738. 3. International Organisation for Standardisation: Milk and
Absolute Quantifi cation of the Lower Abundance Proteome Through Immunoaffi nity Depletion of the Twenty Most Abundant Proteins in Human Serum
d a n c e 0 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 y 6 +1 685.5 b 2 +1 276.1 y 4 +1 500.4 y 3 +1 387.3 y 5
Cathepsin B Detection Kit
excitation at 550 nm, emission >610 nm), cells may be viewed immediately after staining, without a wash step - go to Step 16. d. If viewing under blue (480 nm) excitation and green (540-550 nm) emission
H2A.X (Ser139) Dual Detect CELISA Assay Kit (Fluorogenic Detection)
direct light. A pink or rosy color may develop in the wells. 3. Read the plate using a fluorescence plate reader capable of excitation at 550 nm and emission at 590 nm to detect the HRP signal.
Product Information Sheet - CS0370
35 mm petri dish or onto chamber slides. 3. Grow the cells using your culture media formulation until about 80% confluent. This usually takes about 24 hours, but will vary with your cell line.
Product Information Sheet - MAK497
(µM) 1 Standard #1 10 µL 90 µL 40 2 Standard #2 10 µL 90 µL 24 3 Standard #3 10 µL
Product Information Sheet - CS0360
35 mm petri dish or onto chamber slides. 3. Grow the cells using your culture media formulation until about 80% confluent. This usually takes about 24 hours, but will vary with your cell line.
Product Information Sheet - CS0350
35 mm petri dish or onto chamber slides. 3. Grow the cells using your culture media formulation until about 80% confluent. This usually takes about 24 hours, but will vary with your cell line.
Data Sheet - SAB4200810
2003). 8. Rosseli, S. et al., Traffic, 5, 37-44 (2004). 9. Rosseli, S. et al., Mol. Cell Biol., 24, 550- 560 (2004). AI,DR,OKF,MAM 08/18-1 2018 Sigma-Aldrich Co. LLC. All rights reserved
Data Sheet - P0372
2003). 8. Rosseli, S., et al., Traffic, 5, 37-44 (2004). 9. Rosseli, S., et al., Mol. Cell Biol., 24, 550- 560 (2004). KAA/RM 07/04
Product Information Sheet - CY0100
reduction a sharp absorption peak is observed at 550 nm. The reduction of cytochrome c is monitored by the increase of cytochrome c absorbance at 550 nm. Note that the monitored wavelength is critical
Cytochrome P450 human (C3735)
cycles. 3. Store aliquots at −70 °C. Storage/Stability The product is shipped on dry ice and should be stored at −70 °C. The product as supplied is stable for at least 24 months.
Cytochrome P450 human (C3860)
freeze–thaw cycles. 3. Store aliquots at −70 °C. Storage/Stability The product is shipped on dry ice and should be stored at −70 °C. The product as supplied is stable for at least 24 months.
Data Sheet - 84886
13; 113-120 6. Taplin D., 1965, The use of gentamicin in mycology. - J. Invest. Dermat., 45; 549-550
Product Information Sheet - M3895
Biochem. J., 331(Pt 1), 193-199 (1998). 8. Jiang, W. et al., Biochem. Biophys. Res. Comm., 251(2), 550-556 (1998). 9. Jiang, W. et al., Eur. J. Biochem., 267(8), 2208- 2217 (2000). 10. Madsen, J.B
Product Information Sheet - MAK522
mak522pis Rev 04/24 3 Measurement 1. Incubate for 30 mins at Room Temperature. 2. Read the optical density at 570nm
Data Sheet - 70437
Appl. Oryst., 24, 409-411, 1991. 2. Crystallization of nucleic acids and proteins, A. Ducruix and R. Giege eds., The Practical Approach Series, Oxford Univ. Press, 1992. 3. Current approaches
Bulletin - NA2020
blood, 40 μL of RNase A, and 550 μL of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 μL of 95–100% ethanol and mix. Load onto the binding column (3 × 600 μL) and
Product Information Sheet - NA2010
blood, 40 μL of RNase A, and 550 μL of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 μL of 95–100% ethanol and mix. Load onto the binding column (3 × 600 μL) and
Bulletin - NA2000
blood, 40 mL of RNase A, and 550 mL of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 mL of 95–100% ethanol and mix. Load onto the binding column (3 5 600 mL) and
RAT ANTI-DOPAMINE TRANSPORTER MONOCLONAL ANTIBODY
Neurology (2005) 498:821-839. Jakowec, M.W., et al., J of Neuroscience Research (2004) 76:539-550. Fisher, B.E., et al., J of Neuroscience Research (2004) 77:378-390. Sonntag, K., et al.
RAT ANTI-DOPAMINE TRANSPORTER MONOCLONAL ANTIBODY
freeze/thaw cycles. REFERENCES: Jakowec, M.W., et al., J of Neuroscience Research (2004) 76:539-550. Fisher, B.E., et al., J of Neuroscience Research (2004) 77:378-390. Sonntag, K., et al.
Bulletin - N2020
blood, 40 µl of RNase A, and 550 µl of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 µl of 95-100% ethanol and mix. Load onto the binding column (3 x 600 µl) and spin
TB029
3573 FspI 3 1129 1227 3361 GdiII 4 170 302 710 3527 HaeI 7 691 763 820 1217 2261 2272 2724 HaeII 10 188 209 269 323 550 980 1419 1502 2124 2494 HaeIII 20 HgaI 11 HgiEII 1 2832 HhaI 28 Hin4I 3 912 3138
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