cytochrome c oxidase activity
The absorption of cytochrome c at 550 nm changes
with its oxidation state. This property is the basis for the
assay.3 Cytochrome c is reduced with dithiothreitol and
then reoxidized
by the MPN technique, J. Food Prot. 55, 266 (1992)
2. Hajna and Perry, Am. 1 Publ. Health, 33:550 (1943)
3. Tennant et al., Can. J. Microbiol., 1:733 (1961)
4. Fishbein and Surkiewicz, Appl. Microbiol
1. Hajna A.A., a. Perry C.A., 1943, Am. J. Pbl. Hlth. 33:550-556.
2. Perry, C. A. and Hajna, A. A. (1944) Amer. J. Pub. Hlth. 34, 735-738.
3. International Organisation for Standardisation: Milk and
excitation at 550
nm, emission >610 nm), cells may be viewed immediately after staining, without a wash step - go to Step
16.
d. If viewing under blue (480 nm) excitation and green (540-550 nm) emission
direct light. A pink or rosy color may develop in the wells.
3. Read the plate using a fluorescence plate reader capable of excitation at 550 nm and
emission at 590 nm to detect the HRP signal.
35 mm petri dish or onto chamber slides.
3. Grow the cells using your culture media
formulation until about 80% confluent. This usually
takes about 24 hours, but will vary with your cell
line.
35 mm petri dish or onto chamber slides.
3. Grow the cells using your culture media
formulation until about 80% confluent. This usually
takes about 24 hours, but will vary with your cell
line.
35 mm petri dish or onto chamber slides.
3. Grow the cells using your culture media
formulation until about 80% confluent. This usually
takes about 24 hours, but will vary with your cell
line.
reduction a sharp absorption peak is observed at
550 nm. The reduction of cytochrome c is monitored by
the increase of cytochrome c absorbance at 550 nm.
Note that the monitored wavelength is critical
cycles.
3. Store aliquots at −70 °C.
Storage/Stability
The product is shipped on dry ice and should be stored
at −70 °C. The product as supplied is stable for at least
24 months.
freeze–thaw
cycles.
3. Store aliquots at −70 °C.
Storage/Stability
The product is shipped on dry ice and should be stored
at −70 °C. The product as supplied is stable for at least
24 months.
Appl.
Oryst., 24, 409-411, 1991.
2. Crystallization of nucleic acids and proteins, A. Ducruix and R. Giege eds., The Practical Approach Series, Oxford
Univ. Press, 1992.
3. Current approaches
blood,
40 μL of RNase A, and 550 μL of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 μL of
95–100% ethanol and mix. Load onto the binding column (3 × 600 μL) and
blood,
40 mL of RNase A, and 550 mL of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 mL of
95–100% ethanol and mix. Load onto the binding column (3 5 600 mL) and
blood,
40 μL of RNase A, and 550 μL of Lysis Solution C. Mix and incubate at 55 °C for 10 minutes. Add 550 μL of
95–100% ethanol and mix. Load onto the binding column (3 × 600 μL) and
Neurology (2005) 498:821-839.
Jakowec, M.W., et al., J of Neuroscience Research (2004) 76:539-550.
Fisher, B.E., et al., J of Neuroscience Research (2004) 77:378-390.
Sonntag, K., et al.
freeze/thaw cycles.
REFERENCES: Jakowec, M.W., et al., J of Neuroscience Research (2004) 76:539-550.
Fisher, B.E., et al., J of Neuroscience Research (2004) 77:378-390.
Sonntag, K., et al.
blood,
40 µl of RNase A, and 550 µl of Lysis Solution C. Mix
and incubate at 55 °C for 10 minutes. Add 550 µl of
95-100% ethanol and mix. Load onto the binding
column (3 x 600 µl) and spin