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Determination of Dye to Protein Molar Ratio
(D/P ratio)
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelength
Determination of dye to protein molar ratio (D/P
ratio).
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelengths
Determination of Dye to Protein Molar Ratio (D/P
ratio)
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelength
Determination of Dye to Protein Molar Ratio
(D/P ratio)
1. Measure the Cy3 and Cy5 absorbance at 552 nm
and 650 nm, respectively. Read the absorbance of
the dyes at their exact absorbance wavelength
Sawada, J. et al. (1995) Jpn. J. Cancer Res. 86. 10-12.
8 Yamada, K. et al. (1995) Genomics 29, 552-553.
9 Yamaguchi, N. (1996) Applied and Environment Microbiology 62, 275-278.
10 Kusakabe, A. et al
panel).
Figure 9. Stability of expression over passage. The upper panel shows the
percentage of cells expressing a mean peak current >500 pA at –120 mV at cell passages
12, 16, 23, 27, 31, 34, and 40