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maintenance of germline competent mouse embryonic stem cells.
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R-phycoerythrin as an orange
signal (575 nm peak fluorescence) by the FL2 detector through a band
pass filter (585 +/-21 nm) using logarithmic amplification.
24
d.
R-phycoerythrin as an orange
signal (575 nm peak fluorescence) by the FL2 detector through a band
pass filter (585 +/-21 nm) using logarithmic amplification.
d. In the tricolor protocol, measure Cy-Chrom as a
P6959 M n/d n/d y
583 Protein Kinase Cβ2 P3203 P n/d n/d y
584 Protein Kinase Cβ2 P2584 M n/d n/d y
585 Protein Kinase Cγ P8083 M n/d n/d y
586 Protein Kinase Cδ P8333 P n/d n/d y
587 Protein Kinase Cε
In healthy cells, the dye
accumulates and aggregates in the mitochondria and
fluoresces red (l em = 585-590 nm). During apoptosis,
the electrochemical gradient across the mitochondrial
membrane breaks down
25023 Prkcb1 NP_036845.2 P n/d n/d y
584 Protein Kinase Cβ2 P2584 25023 Prkcb1 NP_036845.2 M n/d n/d y
585 Protein Kinase Cγ P8083 24681 Prkcc NP_036760.1 M n/d n/d y
586 Protein Kinase Cδ P8333 170538 Prkcd
25023 Prkcb1 NP_036845.2 P n/d n/d y
584 Protein Kinase Cβ2 P2584 25023 Prkcb1 NP_036845.2 M n/d n/d y
585 Protein Kinase Cγ P8083 24681 Prkcc NP_036760.1 M n/d n/d y
586 Protein Kinase Cδ P8333 170538 Prkcd